INT282572

Context Info Confidence 0.70 First Reported 2009 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 24 Total Number 25 Disease Relevance 5.80 Pain Relevance 1.13

cytosol (POU5F1) nucleoplasm (POU5F1) nucleus (POU5F1) DNA binding (POU5F1) transcription factor binding (POU5F1) cytoplasm (POU5F1)

Anatomy Link Frequency neural 2 dermis 2 embryos 1 brain 1 external 1 POU5F1 (Homo sapiens)

Pain Link Frequency Relevance Heat Action potential 40 99.92 Inflammation 204 99.24 Potency 51 99.20 pain pelvic 10 96.92 Dismenorea 10 94.36 endometriosis 15 93.56 anesthesia 13 90.08 Sciatic nerve 39 87.92 withdrawal 13 50.48 tetrodotoxin 36 23.84

Disease Link Frequency Relevance Heat Stress 38 99.68 Keloid Scars 412 99.62 INFLAMMATION 216 99.24 Reprotox - General 3 10 96.92 Injury 44 94.72 Reprotox - General 1 10 94.36 Endometriosis (extended) 25 93.56 Hypoxia 39 93.48 Infertility 20 92.60 Benign Tumor 68 91.52

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We found that human endometrium contains potentially pluripotent OCT-4-expressing cells with increased OCT-4 mRNA levels observed in 70% of samples.

Positive_regulation (increased) of OCT-4 mRNA in endometrium

1) Confidence 0.70 Published 2010 Journal Reprod Biol Endocrinol Section Body Doc Link PMC2867815 Disease Relevance 0 Pain Relevance 0

Comparing the number of samples with increased OCT-4 mRNA levels in the follicular and luteal phases, we found no statistically significant difference (35/49 [71%] vs. 27/40 [68%], respectively (p = 0.9).

Positive_regulation (increased) of OCT-4 mRNA

2) Confidence 0.70 Published 2010 Journal Reprod Biol Endocrinol Section Body Doc Link PMC2867815 Disease Relevance 0.45 Pain Relevance 0.23

Moreover, most of the Oct4 target genes were upregulated including Rex1, Nanog, and Sox2 (Fig. 1E).

Positive_regulation (upregulated) of Oct4

3) Confidence 0.62 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.27 Pain Relevance 0.04

After neural induction in differentiated Oct4/ATSCs, we observed an extreme upregulation of TuJ and MAP2ab and low levels of Nestin expression in Oct4/ATSCs after neural differentiation and also neurosphere formation efficiency was higher than control ATSCs (Fig. 6A).

Positive_regulation (induction) of Oct4 in neural

4) Confidence 0.62 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0 Pain Relevance 0.05

During embryogenesis, OCT-4 is initially active as a maternal factor in the oocyte and remains active in embryos throughout the preimplantation period.

Positive_regulation (active) of OCT-4 in embryos

5) Confidence 0.47 Published 2010 Journal Reprod Biol Endocrinol Section Body Doc Link PMC2867815 Disease Relevance 0.44 Pain Relevance 0

Increased OCT-4 mRNA levels were observed in 62/89 (70%) samples.

Positive_regulation (Increased) of OCT-4 mRNA

6) Confidence 0.47 Published 2010 Journal Reprod Biol Endocrinol Section Body Doc Link PMC2867815 Disease Relevance 0.47 Pain Relevance 0.24

Increased OCT-4 mRNA levels in the follicular and luteal phases was found in 35/49 (71%) and 27/40 (68%) of women, respectively (p = 0.9).

Positive_regulation (Increased) of OCT-4 mRNA

7) Confidence 0.47 Published 2010 Journal Reprod Biol Endocrinol Section Abstract Doc Link PMC2867815 Disease Relevance 0 Pain Relevance 0

According to this protocol, hGFs were first infected with a lentivirus to express the mouse Slc7a1 gene (by a plasmid vector from Addgene) and then infected with retroviruses coding human c-MYC, OCT3/4, SOX2 and KLF4 genes for iPS cell induction.

Positive_regulation (induction) of OCT3

8) Confidence 0.45 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2939066 Disease Relevance 0 Pain Relevance 0

Moreover, in in vivo lesion sites of injured brains, TuJ-positive neuron subpopulations generally survived or actively trans-differentiated into neurons after Oct4/ATSC engraftment (Fig. 7A).

Positive_regulation (engraftment) of Oct4 in neuron

9) Confidence 0.45 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.25 Pain Relevance 0.04

Extremely down-regulated Nestin expression was observed in Oct4-induced neural cells after induction (data not showed).

Positive_regulation (observed) of Oct4 in neural

10) Confidence 0.45 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.25 Pain Relevance 0.05

Electrophysiological evaluation (evoked action potential of engrafted Oct4/ATSCs was performed before, immediately after and 30 days after the sciatic taxonomy and Oct4/ATSCs transplantation.

Positive_regulation (engrafted) of Oct4 associated with action potential

11) Confidence 0.45 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0 Pain Relevance 0.28

Engrafted Oct4/ATSCs presented highly neurogenic behavior in the fetal mouse brain 2 months after engraftment (Fig. 1F).

Positive_regulation (Engrafted) of Oct4 in brain

12) Confidence 0.45 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.31 Pain Relevance 0.05

The proliferation-associated signal pathway with a high level of TERT activity and conserved telomere length occurring in Oct4/ATSCs and their gene expression revealed a reversion toward a more immature phenotype.

Positive_regulation (occurring) of Oct4 in telomere

13) Confidence 0.45 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.27 Pain Relevance 0

The cells stained for Ki67 that are negative for OCT4 and MAGE-A4 represent proliferating Sertoli cells (orange arrowhead).

Positive_regulation (negative) of OCT4 in Sertoli cells

14) Confidence 0.44 Published 2010 Journal Human Reproduction (Oxford, England) Section Body Doc Link PMC2939754 Disease Relevance 0 Pain Relevance 0

This demonstrated that a proportion of all three subpopulations of germ cells (OCT4+/MAGE-A4?

Positive_regulation (subpopulations) of OCT4 in germ cells

15) Confidence 0.44 Published 2010 Journal Human Reproduction (Oxford, England) Section Body Doc Link PMC2939754 Disease Relevance 0 Pain Relevance 0

This revealed that the upregulation of key pluripotency-controlling genes, OCT4, SOX2, and NANOG, and ES-associated genes KLF4, CEBP, FOXD1, HMGA1, and DNER.

Positive_regulation (upregulation) of OCT4

16) Confidence 0.42 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0 Pain Relevance 0

MiRNAs highly differentially expressed in Oct4/ATSCs were as follows; let 7, mir 170, mir 132 (upregulated in Oct4/ATSCs) and let 17 and let 120 (downregulated in Oct4/ATSCs).

Positive_regulation (upregulated) of Oct4

17) Confidence 0.42 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.06 Pain Relevance 0

siRNA transfection prominently induced downregulated stemness genes expression, such as Rex1, Sox2, Oct4, and Klf4 with prominent growth attenuation (Figure 3E, 3F).

Positive_regulation (induced) of Oct4

18) Confidence 0.41 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2817727 Disease Relevance 0.49 Pain Relevance 0

Following the Oct4 knockdown experiment, Oct4 was shown to be actively involved in cell proliferation and survival against external chemical stresses after exogenic Oct4 induction (Fig. 2B).

Positive_regulation (induction) of Oct4 in external associated with stress

19) Confidence 0.36 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.37 Pain Relevance 0

A comparison of the expression of these showed that the cell proliferation-associated genes were prominently upregulated in Oct4/ATSCs (Table S1, 39%).

Positive_regulation (upregulated) of Oct4

20) Confidence 0.36 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.31 Pain Relevance 0

General Comments

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