INT282593
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
This work was soon supplemented by the generation of human iPSCs by two groups, one of whom [186] showed that adult human dermal fibroblasts can also be reprogrammed by overexpression of OCT4, SOX2, KLF4, and c-MYC, while another [188] made use of a slightly different set of factors (OCT4, SOX2, LIN28, and NANOG) to reprogram both fetal and adult human fibroblasts. | |||||||||||||||
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This process, which is held as one of the most seminal discoveries in the stem cell field, was first reported by Yamanaka's group [187] and involves overexpression of four key genes (Oct4, Sox2, Klf4, and c-Myc) in murine fibroblasts, resulting in their conversion into cells that resemble ESCs in terms of morphology, gene expression, growth, and differentiation capabilities and are now named induced pluripotent stem cells (iPSCs). | |||||||||||||||
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Since these initial papers there has been remarkable progress in the field, aimed largely at increasing the efficiency of the iPSC generation protocol and replacing the initial retroviral vectors that were used to transfect fibroblasts with OCT4, SOX2, KLF4, and c-MYC. | |||||||||||||||
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We evaluated the expression of Oct4 (POU5F1) to determine whether exogenic Oct4 induced the expression of early developmental genes KLF4, Sox-2, Rex-1, Utf1, Dapp5, FGF4, ERas, and Nanog in cultured ATSCs (Fig. 1E). | |||||||||||||||
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Krüppel-like factor 4 (KLF4), a transcription factor highly expressed in normal human intestine and critical for intestinal differentiation, inhibits Wnt signaling by direct interaction with the C-terminal transactivation domain of ? | |||||||||||||||
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Global gene expression analysis results showed that Oct4 regulated target genes which could be characterized as differentially regulated genes such as pluripotency markers NANOG, SOX2, and KLF4 and markers of undifferentiated stem cells FOXD1, CDC2, and EPHB1. | |||||||||||||||
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siRNA transfection prominently induced downregulated stemness genes expression, such as Rex1, Sox2, Oct4, and Klf4 with prominent growth attenuation (Figure 3E, 3F).
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Indeed, iPSs are embryonic-like SCs derived from somatic cells by forced expression of reprogramming factors (Oct3/4 and Sox2 along with either Klf4 or Nanog and Lin28). | |||||||||||||||
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