INT282593

Context Info Confidence 0.61 First Reported 2009 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 8 Total Number 8 Disease Relevance 1.09 Pain Relevance 0.09

nucleolus (KLF4) nucleus (KLF4) intracellular (KLF4) DNA binding (KLF4)

Anatomy Link Frequency fibroblasts 3 somatic cells 1 stem cells 1 intestine 1 KLF4 (Homo sapiens)

Pain Link Frequency Relevance Heat Potency 12 77.20 Bile 2 53.96 Action potential 10 53.28 Spinal cord 15 5.00 Inflammation 10 5.00 Sciatic nerve 9 5.00 adenocard 9 5.00 aspirin 7 5.00 tetrodotoxin 6 5.00 antagonist 4 5.00

Disease Link Frequency Relevance Heat Hypoxia 35 92.00 Teratocarcinoma 2 84.84 Teratoma 8 84.20 Injury 16 82.88 Obesity 21 75.00 Wound Healing 3 72.64 Aging 22 68.84 Retinal Diseases 33 62.76 Severe Combined Immunodeficiency 12 62.24 Liver Disease 10 50.00

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This work was soon supplemented by the generation of human iPSCs by two groups, one of whom [186] showed that adult human dermal fibroblasts can also be reprogrammed by overexpression of OCT4, SOX2, KLF4, and c-MYC, while another [188] made use of a slightly different set of factors (OCT4, SOX2, LIN28, and NANOG) to reprogram both fetal and adult human fibroblasts.

Gene_expression (overexpression) of KLF4 in fibroblasts

1) Confidence 0.61 Published 2009 Journal Stem Cells (Dayton, Ohio) Section Body Doc Link PMC2962903 Disease Relevance 0 Pain Relevance 0

This process, which is held as one of the most seminal discoveries in the stem cell field, was first reported by Yamanaka's group [187] and involves overexpression of four key genes (Oct4, Sox2, Klf4, and c-Myc) in murine fibroblasts, resulting in their conversion into cells that resemble ESCs in terms of morphology, gene expression, growth, and differentiation capabilities and are now named induced pluripotent stem cells (iPSCs).

Gene_expression (overexpression) of Klf4 in fibroblasts

2) Confidence 0.61 Published 2009 Journal Stem Cells (Dayton, Ohio) Section Body Doc Link PMC2962903 Disease Relevance 0.06 Pain Relevance 0

Since these initial papers there has been remarkable progress in the field, aimed largely at increasing the efficiency of the iPSC generation protocol and replacing the initial retroviral vectors that were used to transfect fibroblasts with OCT4, SOX2, KLF4, and c-MYC.

Gene_expression (transfect) of KLF4 in fibroblasts

3) Confidence 0.53 Published 2009 Journal Stem Cells (Dayton, Ohio) Section Body Doc Link PMC2962903 Disease Relevance 0 Pain Relevance 0

We evaluated the expression of Oct4 (POU5F1) to determine whether exogenic Oct4 induced the expression of early developmental genes KLF4, Sox-2, Rex-1, Utf1, Dapp5, FGF4, ERas, and Nanog in cultured ATSCs (Fig. 1E).

Gene_expression (expression) of KLF4

4) Confidence 0.33 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.24 Pain Relevance 0.04

Krüppel-like factor 4 (KLF4), a transcription factor highly expressed in normal human intestine and critical for intestinal differentiation, inhibits Wnt signaling by direct interaction with the C-terminal transactivation domain of ?

Gene_expression (expressed) of KLF4 in intestine

5) Confidence 0.31 Published 2010 Journal Mol Cancer Section Body Doc Link PMC2955614 Disease Relevance 0 Pain Relevance 0

Global gene expression analysis results showed that Oct4 regulated target genes which could be characterized as differentially regulated genes such as pluripotency markers NANOG, SOX2, and KLF4 and markers of undifferentiated stem cells FOXD1, CDC2, and EPHB1.

Gene_expression (markers) of KLF4 in stem cells

6) Confidence 0.29 Published 2009 Journal PLoS ONE Section Abstract Doc Link PMC2747014 Disease Relevance 0.13 Pain Relevance 0.03

siRNA transfection prominently induced downregulated stemness genes expression, such as Rex1, Sox2, Oct4, and Klf4 with prominent growth attenuation (Figure 3E, 3F).

Gene_expression (expression) of Klf4

7) Confidence 0.24 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2817727 Disease Relevance 0.48 Pain Relevance 0

Indeed, iPSs are embryonic-like SCs derived from somatic cells by forced expression of reprogramming factors (Oct3/4 and Sox2 along with either Klf4 or Nanog and Lin28).

Gene_expression (expression) of Klf4 in somatic cells

8) Confidence 0.20 Published 2010 Journal Stem Cells International Section Body Doc Link PMC2963137 Disease Relevance 0.17 Pain Relevance 0.03

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