INT282599

Context Info Confidence 0.62 First Reported 2009 Last Reported 2010 Negated 1 Speculated 2 Reported most in Body Documents 10 Total Number 16 Disease Relevance 4.89 Pain Relevance 0.71

cytosol (POU5F1) nucleoplasm (POU5F1) nucleus (POU5F1) DNA binding (POU5F1) transcription factor binding (POU5F1) cytoplasm (POU5F1)

Anatomy Link Frequency endometrium 4 fat 2 stem cell 2 POU5F1 (Homo sapiens)

Pain Link Frequency Relevance Heat endometriosis 15 99.40 cytokine 135 79.72 Inflammation 255 73.60 pain pelvic 10 69.76 Dismenorea 10 67.20 Hippocampus 8 61.24 Action potential 20 25.00 Potency 22 9.92 Spinal cord 24 5.00 chemokine 20 5.00

Disease Link Frequency Relevance Heat Keloid Scars 515 99.50 Endometriosis (extended) 25 99.40 Obesity 38 99.12 Apoptosis 46 97.28 Death 28 94.44 Osteoporosis 6 90.32 Disease 92 88.40 Hypersensitivity 10 88.04 Targeted Disruption 8 79.12 Diabetes Mellitus 24 77.40

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Thus, other yet unknown factors influencing the expression of OCT-4 may be present in human endometrium.

Regulation (influencing) of Gene_expression (expression) of OCT-4 in endometrium

1) Confidence 0.62 Published 2010 Journal Reprod Biol Endocrinol Section Body Doc Link PMC2867815 Disease Relevance 0.13 Pain Relevance 0.03

To clarify the role of the POU domain transcription factor Oct4 in Adipose Tissue Stromal Cells (ATSCs), we investigated the regulation of Oct4 expression and other embryonic genes in fully differentiated cells, in addition to identifying expression at the gene and protein levels.

Spec (investigated) Regulation (regulation) of Gene_expression (expression) of Oct4 associated with obesity

2) Confidence 0.55 Published 2009 Journal PLoS ONE Section Abstract Doc Link PMC2747014 Disease Relevance 0.17 Pain Relevance 0

When Oct4 expression was recovered by exogenic Oct4 gene overexpression in damaged cells, ATSCs recovered to normal and viable cell with cell survival related signal pathway molecule such as Akt/PI3K, Bcl2, and ERK1/2.

Regulation (recovered) of Gene_expression (expression) of Oct4

3) Confidence 0.40 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.29 Pain Relevance 0

We studied the effect of Oct4 overexpression on mesodermal lineage differentiation into fat, bone, and chondrocyte of ATSCs.

Regulation (effect) of Gene_expression (overexpression) of Oct4 in fat

4) Confidence 0.40 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.09 Pain Relevance 0

Our results show that ATSC can undergo an increase in developmental potential following reprogramming via the overexpression of the embryonic transcription factor, Rex1, Oct4, and Oct4-dependent Nanog and Sox2.

Regulation (dependent) of Gene_expression (overexpression) of Oct4

5) Confidence 0.37 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2817727 Disease Relevance 0.26 Pain Relevance 0

Our results show that ATSC can undergo an increase in developmental potential following reprogramming via the overexpression of the embryonic transcription factor, Rex1, Oct4, and Oct4-dependent Nanog and Sox2.

Regulation (dependent) of Gene_expression (overexpression) of Oct4

6) Confidence 0.37 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2817727 Disease Relevance 0.26 Pain Relevance 0

In a univariate regression model, the day of the menstrual cycle, using 3-day intervals, did not influence OCT-4 protein expression, when comparing an IRS <6 vs. ?

Neg (not) Regulation (influence) of Gene_expression (expression) of OCT-4 protein

7) Confidence 0.27 Published 2010 Journal Reprod Biol Endocrinol Section Body Doc Link PMC2867815 Disease Relevance 0.33 Pain Relevance 0.17

Thus, we can only rule out a profound influence of menstrual cycle-dependent changes of the human endometrium on OCT-4 expression.

Regulation (changes) of Gene_expression (expression) of OCT-4 in endometrium

8) Confidence 0.27 Published 2010 Journal Reprod Biol Endocrinol Section Body Doc Link PMC2867815 Disease Relevance 0.13 Pain Relevance 0.03

Also, differential regulation of OCT-4 expression in women with endometriosis may be a worthwile target of investigation.

Regulation (regulation) of Gene_expression (expression) of OCT-4 associated with endometriosis

9) Confidence 0.27 Published 2010 Journal Reprod Biol Endocrinol Section Body Doc Link PMC2867815 Disease Relevance 0.15 Pain Relevance 0.05

Menstrual cycle-dependency of OCT-4 expression has not been investigated to date.

Regulation (dependency) of Gene_expression (expression) of OCT-4

10) Confidence 0.27 Published 2010 Journal Reprod Biol Endocrinol Section Abstract Doc Link PMC2867815 Disease Relevance 0 Pain Relevance 0

To clarify the role of Oct4 in adult cells, we investigated the regulation of Oct4 expression and other embryonic genes in fully differentiated cells, while identifying that it was also expressed at the gene and protein levels in ATSCs.

Spec (investigated) Regulation (regulation) of Gene_expression (expression) of Oct4

11) Confidence 0.24 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2747014 Disease Relevance 0.08 Pain Relevance 0.03

Furthermore, we demonstrated that blocking IL-6 abolished IL-17-induced up-regulation of hTERT and Oct-4 expression; however, blocking IL-17 failed to attenuate IL-6-induced up-regulation of hTERT and Oct-4 expression in KPCs.

Regulation (regulation) of Gene_expression (expression) of Oct-4

12) Confidence 0.18 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2771422 Disease Relevance 1.23 Pain Relevance 0.20

However, IL-6-induced up-regulation of hTERT and Oct-4 expression was only negated by pre-treatment with IL-6 neutralizing antibody, not by IL-17 neutralizing antibody (Figure 5E).

Regulation (regulation) of Gene_expression (expression) of Oct-4

13) Confidence 0.13 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2771422 Disease Relevance 0.12 Pain Relevance 0

To further delineate the relationship of IL-6 and IL-17 in the regulation of hTERT and Oct-4 expression in SKPs and KPCs, we pretreated cells with neutralizing antibody for either IL-17, or IL-6, or both followed by IL-17 exposure.

Regulation (regulation) of Gene_expression (expression) of Oct-4

14) Confidence 0.13 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2771422 Disease Relevance 0.08 Pain Relevance 0

Furthermore, we demonstrated that blocking IL-6 abolished IL-17-induced up-regulation of hTERT and Oct-4 expression; however, blocking IL-17 failed to attenuate IL-6-induced up-regulation of hTERT and Oct-4 expression in KPCs.

Regulation (regulation) of Gene_expression (expression) of Oct-4

15) Confidence 0.13 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2771422 Disease Relevance 1.12 Pain Relevance 0.20

All together, our results suggest that the IL-17/IL-6 axis may function to maintain the highly elevated IL-6 level in the distinct keloid niche with abilities to regulate key stem cell functions such as hTERT and Oct-4 expression, thus contributing to the formation of the benign keloid growth.

Regulation (regulate) of Gene_expression (expression) of Oct-4 in stem cell associated with keloid scars

16) Confidence 0.08 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2771422 Disease Relevance 0.45 Pain Relevance 0

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