INT284265

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Context Info
Confidence 0.64
First Reported 2009
Last Reported 2011
Negated 1
Speculated 1
Reported most in Body
Documents 29
Total Number 32
Disease Relevance 10.70
Pain Relevance 2.84

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
macrophages 8
THP-1 3
HeLa 2
hematopoietic cells 2
SH2 1
Mir155 (Mus musculus)
Pain Link Frequency Relevance Heat
Multiple sclerosis 474 99.80 Very High Very High Very High
Osteoarthritis 2 97.76 Very High Very High Very High
rheumatoid arthritis 180 97.64 Very High Very High Very High
Inflammatory response 282 95.04 Very High Very High Very High
cytokine 223 94.32 High High
Central nervous system 83 87.92 High High
Inflammation 456 85.64 High High
Inflammatory mediators 25 77.16 Quite High
chemokine 79 75.96 Quite High
Arthritis 238 66.24 Quite High
Disease Link Frequency Relevance Heat
Repression 252 100.00 Very High Very High Very High
Demyelinating Disease 525 99.80 Very High Very High Very High
Myeloid Leukemia 168 98.88 Very High Very High Very High
Leukemia 54 98.40 Very High Very High Very High
Acute-phase Reaction 44 97.92 Very High Very High Very High
Osteoarthritis 2 97.76 Very High Very High Very High
Rheumatoid Arthritis 180 97.64 Very High Very High Very High
Disease 400 97.32 Very High Very High Very High
INFLAMMATION 733 95.04 Very High Very High Very High
Myeloproliferative Disorder 66 92.32 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Since miR-155 has previously been shown to be upregulated during macrophage activation (8–10) we first investigated the expression of miR-155 in murine Raw264.7 macrophage cells upon LPS stimulation.
Spec (investigated) Gene_expression (expression) of miR-155 in macrophage
1) Confidence 0.64 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.06 Pain Relevance 0
Intraperitoneal administration of LPS significantly induced the expression of miR-155 in splenocytes 2 h post-treatment, thereby corroborating our results obtained with LPS-stimulated murine macrophages (Figure 4B).
Gene_expression (expression) of miR-155 in macrophages
2) Confidence 0.64 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0 Pain Relevance 0
Taken together, our data suggest that the levels of individual c/ebp Beta isoforms are regulated by ectopically expressed miR-155.


Gene_expression (expressed) of miR-155
3) Confidence 0.64 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.32 Pain Relevance 0
In this study, we show that c/ebp Beta is a bona fide target of miR-155 and that LPS treatment of murine macrophages strongly induces miR-155 expression, leading to translational repression of c/ebp Beta.
Gene_expression (expression) of miR-155 in macrophages associated with repression
4) Confidence 0.64 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.49 Pain Relevance 0.23
Our observations are in good agreement with recent reports, showing that ectopically expressed miR-155 can target c/ebp Beta in vitro (9,24).
Gene_expression (expressed) of miR-155
5) Confidence 0.64 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.51 Pain Relevance 0.24
UTR of the Renilla luciferase gene showed a concentration-dependent repression of the luciferase reporter, which correlated with the increased expression of miR-155 in LPS-treated mouse Raw264.7 macrophages (Figure 1B).
Gene_expression (expression) of miR-155 in macrophages associated with repression
6) Confidence 0.64 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.10 Pain Relevance 0
The LNA-antimiR compound effectively antagonizes miR-155 expression in LPS-treated mice compared to saline treated controls (*** indicates P < 0.001).
Gene_expression (expression) of miR-155
7) Confidence 0.56 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.07 Pain Relevance 0.03
(B) Quantitative RT-PCR analysis of miR-155 expression in splenocytes of mice treated with LNA-antimiR or LNA control by three intravenous injections of 25 mg/kg over three consecutive days.
Gene_expression (expression) of miR-155
8) Confidence 0.56 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.08 Pain Relevance 0.04
Treatment of cultured mouse macrophages with LPS showed dose-dependent induction of miR-155 with more than 10-fold increase in miR-155 expression levels at a concentration of 100 ng/ml LPS after 18 h (Figure 1A).
Gene_expression (expression) of miR-155 in macrophages
9) Confidence 0.56 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.08 Pain Relevance 0
(A) Quantitative RT-PCR analysis of miR-155 expression in Raw264.7 cells stimulated with LPS for 18 h at the indicated concentrations.
Gene_expression (expression) of miR-155
10) Confidence 0.56 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0 Pain Relevance 0
We report here that LPS treatment induces miR-155 expression in cultured mouse macrophages and in mouse splenocytes in vivo and that silencing of miR-155 leads to derepression of its direct target c/ebp Beta in vitro and in vivo.
Gene_expression (expression) of miR-155 in macrophages
11) Confidence 0.56 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.41 Pain Relevance 0.23
We obtained similar results with undifferentiated human monocytic THP-1 cells, in which transfection with pre-miR-155 resulted in repression of the c/ebp Beta isoforms (Figure 2D).
Gene_expression (transfection) of pre-miR-155 in THP-1 associated with repression
12) Confidence 0.56 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.35 Pain Relevance 0
(B) Assessment of luciferase reporter activity in HeLa cells cotransfected with pre-miR-155 in combination with either the Renilla/firefly luciferase psiCHECK2 control, the psiCHECK2 luciferase construct (miR-155 sensor) harbouring the miR-155 perfect match target site, the psiCHECK2 construct harbouring the c/ebp Beta 3?
Gene_expression (cotransfected) of pre-miR-155 in HeLa
13) Confidence 0.56 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.14 Pain Relevance 0
(B) Assessment of luciferase reporter activity in HeLa cells cotransfected with pre-miR-155 in combination with either the Renilla/firefly luciferase psiCHECK2 control, the psiCHECK2 luciferase construct (miR-155 sensor) harbouring the miR-155 perfect match target site, the psiCHECK2 construct harbouring the c/ebp Beta 3?
Gene_expression (harbouring) of miR-155 sensor in HeLa
14) Confidence 0.55 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.13 Pain Relevance 0
The miR-155 target site is indicated in the 3?
Gene_expression (The) of miR-155 target site
15) Confidence 0.55 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.28 Pain Relevance 0.03
The LNA-antimiR-155 showed concentration-dependent silencing of miR-155 in LPS-treated mouse Raw264.7 macrophages as shown by efficient derepression of the miR-155 sensor, whereas the LNA control oligonucleotide had no effect on the luciferase reporter activity at the same concentrations (Figure 1C).
Gene_expression (derepression) of miR-155 sensor in macrophages
16) Confidence 0.55 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.07 Pain Relevance 0
Translational repression of c/ebp Beta isoforms by miR-155
Gene_expression (repression) of miR-155 associated with repression
17) Confidence 0.55 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.20 Pain Relevance 0.05
THP-1 cells were cotransfected with 5 nM pre-miR-155 in combination with either LNA control (5 nM) or LNA-antimiR (5 nM) before LPS stimulation for 6 h.
Gene_expression (cotransfected) of pre-miR-155 in THP-1
18) Confidence 0.48 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0 Pain Relevance 0
We also observed repression of another direct miR-155 target, the Ets family transcription factor Pu.1 in Raw264.7 cells (Figure 2C) (11), implying that both c/ebp Beta and Pu.1 are targeted by miR-155 in the Raw264.7 macrophage model system.
Gene_expression (target) of miR-155 in macrophage associated with repression
19) Confidence 0.48 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.36 Pain Relevance 0
Interestingly, the c/ebp Beta transcript is predicted by several computer algorithms (PicTar, miRanda, TargetScan) to be a miR-155 target due to the presence of a miR-155 target site in its 3?
Gene_expression (target) of miR-155
20) Confidence 0.48 Published 2009 Journal Nucleic Acids Research Section Body Doc Link PMC2761263 Disease Relevance 0.25 Pain Relevance 0.05

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