INT284848

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Context Info
Confidence 0.74
First Reported 2009
Last Reported 2010
Negated 1
Speculated 1
Reported most in Body
Documents 23
Total Number 27
Disease Relevance 1.36
Pain Relevance 3.25

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

extracellular region (Pdzd2) endoplasmic reticulum (Pdzd2) nucleus (Pdzd2)
cytoplasm (Pdzd2)
Anatomy Link Frequency
neurons 13
plasma 3
sensory neurons 1
pancreas 1
lung 1
Pdzd2 (Mus musculus)
Pain Link Frequency Relevance Heat
sodium channel 317 99.88 Very High Very High Very High
nav1.8 23 99.10 Very High Very High Very High
Analgesic 46 94.32 High High
tetrodotoxin 311 88.40 High High
nociceptor 23 75.84 Quite High
withdrawal 46 72.72 Quite High
Pain 147 71.60 Quite High
Acute pain 23 69.04 Quite High
Pain threshold 27 63.04 Quite High
ASIC 4 53.24 Quite High
Disease Link Frequency Relevance Heat
Nociception 85 97.76 Very High Very High Very High
Targeted Disruption 181 85.80 High High
Pain 209 71.60 Quite High
Inflammatory Pain 27 18.80 Low Low
INFLAMMATION 27 11.40 Low Low
Neuropathic Pain 12 5.00 Very Low Very Low Very Low
Diabetic Neuropathy 4 5.00 Very Low Very Low Very Low
Nerve Compression Syndromes 4 5.00 Very Low Very Low Very Low
Herpes Simplex Virus 4 5.00 Very Low Very Low Very Low
Injury 4 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Transfection of Pdzd2-specific siRNA (Pdzd2 siRNA2) into cultured DRG neurons caused distinct down-regulation of Pdzd2 (Fig. 4B).
Gene_expression (Transfection) of Pdzd2 in neurons
1) Confidence 0.74 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.14
The expression of myc-Pdzd2 also did not help translocation of NaV1.8 into the plasma membrane in CHO-SNS22 cells (Fig. 3A).
Gene_expression (expression) of Pdzd2 in plasma
2) Confidence 0.74 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0.05 Pain Relevance 0.07
Negative control siRNA which has no homology to any known mammalian gene failed to downregulate the Pdzd2 expression despite its high transfection efficiency (> 50%), suggesting that Pdzd2 siRNA2 effectively and specifically downregulates Pdzd2 expression in cultured DRG neurons.
Gene_expression (expression) of Pdzd2 in neurons
3) Confidence 0.74 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0.13 Pain Relevance 0.31
Negative control siRNA which has no homology to any known mammalian gene failed to downregulate the Pdzd2 expression despite its high transfection efficiency (> 50%), suggesting that Pdzd2 siRNA2 effectively and specifically downregulates Pdzd2 expression in cultured DRG neurons.
Gene_expression (expression) of Pdzd2 in neurons
4) Confidence 0.74 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0.11 Pain Relevance 0.25
The myc-Pdzd2 expressed in CHO-SNS22 cells did not show specific localisation in the plasma membrane (Fig. 3A) unlike in MDCK cells (Ohno et al., 2002).
Gene_expression (expressed) of Pdzd2 in plasma
5) Confidence 0.74 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0.06 Pain Relevance 0.08
The interaction between NaV1.8 and Pdzd2 in the myc-Pdzd2 transfected CHO-SNS22 cells was confirmed by co-immunoprecipitation (Fig. 3C).


Gene_expression (transfected) of Pdzd2
6) Confidence 0.64 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.12
We tested the possible regulatory role of Pdzd2 on Nav1.8 in sensory neurons by microinjection of the Pdzd2 antisense expression vector, pcDNA3-AS(Pdzd2), with a GFP expression vector pRK7-GFP into the nuclei of cultured DRG neurons.
Gene_expression (expression) of Pdzd2 in neurons associated with nav1.8
7) Confidence 0.64 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.10
Transfection of Pdzd2-specific siRNA (Pdzd2 siRNA2) into cultured DRG neurons caused distinct down-regulation of Pdzd2 (Fig. 4B).
Gene_expression (Transfection) of Pdzd2-specific in neurons
8) Confidence 0.64 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.14
The following siRNA duplexes were used; negative control siRNA, UUCUCCGAACGUGUCACGUdTdT; Pdzd2-specific siRNA, GGCAAGGGCCUUGGCUUUAdTdT (Qiagen).
Gene_expression (siRNA) of Pdzd2-specific
9) Confidence 0.64 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0
The introduction of pcDNA3-AS(Pdzd2) caused a great loss (71%) of the mean peak NaV1.8 current density compared with control neurons injected with pRK7-GFP only (Fig. 4A).
Gene_expression (introduction) of Pdzd2 in neurons
10) Confidence 0.64 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.09
Pdzd2 does not help translocation of NaV1.8 to the plasma membrane in CHO cells
Neg (not) Gene_expression (help) of Pdzd2 in plasma
11) Confidence 0.64 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0.08 Pain Relevance 0.10
We tested the possible regulatory role of Pdzd2 on Nav1.8 in sensory neurons by microinjection of the Pdzd2 antisense expression vector, pcDNA3-AS(Pdzd2), with a GFP expression vector pRK7-GFP into the nuclei of cultured DRG neurons.
Gene_expression (expression) of Pdzd2 in neurons associated with nav1.8
12) Confidence 0.64 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.10
Pdzd2 is essential for functional expression of NaV1.8 current in DRG neurons
Gene_expression (expression) of Pdzd2 in neurons
13) Confidence 0.57 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.11
As majority of peripherin positive DRG neurons express Pdzd2; it is likely that NaV1.8 is co-expressed with Pdzd2 in these cells.
Gene_expression (expressed) of Pdzd2 in neurons
14) Confidence 0.57 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.08
The cDNA for haemagglutinin (HA)-tagged rat Pdzd2 C-terminal fragment (amino acid positions (aa) 2567–2767) was cloned into NcoI/XbaI sites of pBS500 vector in order to produce green fluorescent protein (GFP)-fused Pdzd2 C-terminal polypeptide whose estimated molecular weight is 60 kDa.
Gene_expression (produce) of Pdzd2
15) Confidence 0.57 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0.05 Pain Relevance 0.05
It is also plausible that NaV1.8 itself is compensatorily upregulated in the Pdzd2-deficient mice, because Pdzd2 starts to be expressed earlier (E10.5, K.
Gene_expression (expressed) of Pdzd2
16) Confidence 0.57 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0.29 Pain Relevance 0.18
As majority of peripherin positive DRG neurons express Pdzd2; it is likely that NaV1.8 is co-expressed with Pdzd2 in these cells.
Gene_expression (express) of Pdzd2 in neurons
17) Confidence 0.57 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.08
A complete shutdown of expression of Pdzd2 variants 1, 2 and 5 in the Pdzd2 deficient mice were confirmed by RT-PCR.
Gene_expression (expression) of Pdzd2
18) Confidence 0.57 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.40
The localisation of NaV1.8 in myc-Pdzd2 transfected cells was different from non-transfected cells which showed more distinct staining in organelle (Fig. 3B).
Gene_expression (transfected) of Pdzd2
19) Confidence 0.57 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.12
Pdzd2 is expressed in both large and small diameter sensory neurons
Gene_expression (expressed) of Pdzd2 in sensory neurons
20) Confidence 0.57 Published 2009 Journal Mol Cell Neurosci Section Body Doc Link PMC2764382 Disease Relevance 0 Pain Relevance 0.12

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