INT288483

Context Info Confidence 0.72 First Reported 2009 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 8 Total Number 8 Disease Relevance 4.51 Pain Relevance 0.97

intracellular (Tlr3) cytoplasm (Tlr3)

Anatomy Link Frequency whiskers 3 proximal 1 motor neurons 1 Tlr3 (Rattus norvegicus)

Pain Link Frequency Relevance Heat Central nervous system 6 91.44 spastic colon 127 90.68 Inflammatory mediators 6 81.52 Inflammation 112 81.24 Crohn's disease 1 76.52 cytokine 69 56.60 nud 9 43.36 fifth nerve 18 5.00 isoflurane 13 5.00 trigeminal ganglion 12 5.00

Disease Link Frequency Relevance Heat Viral Meningitis 192 99.62 Stress 60 98.72 Sprains And Strains 294 98.52 Irritable Bowel Syndrome / Irritable Bowel Syndrome Super 126 90.68 Cold Sores 366 86.00 Infection 186 82.20 INFLAMMATION 131 81.60 Disease 25 76.52 Inflammatory Bowel Disease 9 75.76 Viral Infection 6 68.20

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

In peripheral nerves Schwann cells express high levels of TLRs, with TLR3 and -4 being the most prominent, whereas in sensory and motor neurons the levels are minimal [10].

Gene_expression (express) of TLR3 in motor neurons

1) Confidence 0.72 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2929186 Disease Relevance 0.56 Pain Relevance 0.15

Baseline expression of Tlr3 in naïve animals was higher in DA, than in PVG rats.

Gene_expression (expression) of Tlr3

2) Confidence 0.72 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2929186 Disease Relevance 0.27 Pain Relevance 0.04

Tlr3 expressed in cell endosomes recognizes double-stranded RNA.

Gene_expression (expressed) of Tlr3

3) Confidence 0.72 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2929186 Disease Relevance 0.33 Pain Relevance 0.04

However, in our HSE model an elevated expression of Tlr3 in the whiskers area was seen in the susceptible rat and we hypothesized that the susceptibility was not caused by low expression of Tlr3.

Gene_expression (expression) of Tlr3 in whiskers associated with viral meningitis

4) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2929186 Disease Relevance 0.79 Pain Relevance 0.04

The levels of Tlr3 were increased at 12 hpi and 1 dpi in the whiskers area of the DA rats, while differences were significant also at later time points in PVG rats.

Gene_expression (levels) of Tlr3 in whiskers

5) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2929186 Disease Relevance 0.33 Pain Relevance 0.04

However, in our HSE model an elevated expression of Tlr3 in the whiskers area was seen in the susceptible rat and we hypothesized that the susceptibility was not caused by low expression of Tlr3.

Gene_expression (expression) of Tlr3 in whiskers associated with viral meningitis

6) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2929186 Disease Relevance 0.81 Pain Relevance 0.04

Comparing mRNA levels, we see a significant difference in the levels of TLR3, the receptor for dsRNA [40], in the proximal (p?

Gene_expression (levels) of TLR3 in proximal

7) Confidence 0.45 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2785428 Disease Relevance 0.31 Pain Relevance 0

The authors used 2 different models (maternal separation and stress susceptible Wistar-Kyoto rats), showing a significantly higher expression of the TLR3, 4 and 5 on both, while the Wistar-Kyoto which are genetically susceptible to stress, also had a significant over expression in the TLRs 7, 8 and 9 compared to the Sprague-Dawley which are not genetically susceptible to stress.

Gene_expression (expression) of TLR3 associated with stress

8) Confidence 0.37 Published 2010 Journal Journal of Neurogastroenterology and Motility Section Body Doc Link PMC2978389 Disease Relevance 0.57 Pain Relevance 0.62

General Comments

This test has worked.