INT290981

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Context Info
Confidence 0.01
First Reported 2010
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 6
Disease Relevance 0.63
Pain Relevance 0

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

phosphatase activity (Nt5c3) mitochondrion (Nt5c3) endoplasmic reticulum (Nt5c3)
cytoplasm (Nt5c3)
Anatomy Link Frequency
P-1 2
brain 1
Nt5c3 (Mus musculus)
Pain Link Frequency Relevance Heat
Central nervous system 6 42.16 Quite Low
tolerance 24 28.40 Quite Low
cytokine 24 5.00 Very Low Very Low Very Low
chemokine 6 5.00 Very Low Very Low Very Low
Demyelination 6 5.00 Very Low Very Low Very Low
Neuropathic pain 6 5.00 Very Low Very Low Very Low
Inflammation 6 5.00 Very Low Very Low Very Low
Pain 6 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Cytomegalovirus Infection 6 86.88 High High
Infection 198 79.04 Quite High
Encephalitis 90 67.36 Quite High
Viral Infection 12 66.40 Quite High
Rabies Virus Infection 12 60.08 Quite High
Viral Meningitis 6 51.88 Quite High
Spinocerebellar Ataxia Type 2 6 45.92 Quite Low
Brain Tumor 6 44.76 Quite Low
Ebola Virus Infection 6 39.56 Quite Low
Coronavirus Infection 6 38.72 Quite Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
At 24 hr post transfection, cells transfected with both P gene siRNAs (P-1 to P-4) and target expressing plasmid pAcGFP1N1-CHPV-P showed that P-2 siRNA could reduce the GFP expression and P gene expression approximately by 80% (Fig 2a-c c) (p<0.05), whereas P-1, P-3 and P-4 were not very effective.
Gene_expression (expressing) of pAcGFP1N1-CHPV-P in P-1
1) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2797643 Disease Relevance 0 Pain Relevance 0
Different shRNA expressing plasmids (5 µg) were co-transfected individually with plasmid pAcGFP1N1-CHPV-P (500 ng). 24 hr post transfection, cells were harvested, RNA isolated and real time one step RT-PCR and FACS analysis were carried out.
Gene_expression (transfection) of pAcGFP1N1-CHPV-P
2) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2797643 Disease Relevance 0.24 Pain Relevance 0
Total RNA from cells transfected with pAcGFP1N1-CHPV-P, mouse brain samples and viral RNA from supernatant of CHPV infected cells were subjected to real time one step RT-PCR as described earlier [24].
Gene_expression (transfected) of pAcGFP1N1-CHPV-P in brain
3) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2797643 Disease Relevance 0.08 Pain Relevance 0
Cells were transfected with P gene siRNA (P-1 to P-4) at a concentration of 100 pmol and 2 µg of target expressing plasmid pAcGFP1N1-CHPV-P. 24 hr post transfection, cells were harvested and flow cytometry and real time one step RT-PCR were carried out.
Gene_expression (expressing) of pAcGFP1N1-CHPV-P in P-1
4) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2797643 Disease Relevance 0 Pain Relevance 0
Confocal microscopy of HEK-293 cells transfected with pAcGFP1N1 and pAcGFP1N1-CHPV-P showed cytoplasmic localization of fusion protein

P gene siRNA validation with pAcGFP1N1-CHPV-P and CHPV

Gene_expression (transfected) of pAcGFP1N1-CHPV-P
5) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2797643 Disease Relevance 0 Pain Relevance 0
HEK-293 cells were transfected with 2 µg each of pAcGFP1N1 and pAcGFP1N1-CHPV-P.
Gene_expression (transfected) of pAcGFP1N1-CHPV-P
6) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2797643 Disease Relevance 0.32 Pain Relevance 0

General Comments

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