INT29109

Context Info Confidence 0.42 First Reported 1987 Last Reported 2008 Negated 0 Speculated 0 Reported most in Abstract Documents 6 Total Number 8 Disease Relevance 2.90 Pain Relevance 1.07

cytosol (Alox5) nuclear envelope (Alox5) oxidoreductase activity (Alox5) nucleus (Alox5) cytoplasm (Alox5)

Anatomy Link Frequency leukocyte 2 brain 2 cortex 2 neutrophils 2 Alox5 (Rattus norvegicus)

Pain Link Frequency Relevance Heat Kinase C 2 99.40 Inflammatory mediators 12 99.08 Inflammation 74 95.60 ischemia 63 93.52 Bioavailability 12 90.60 Potency 1 85.92 cINOD 5 81.56 Hyperalgesia 1 78.32 Inflammatory response 10 78.28 Pain 2 51.44

Disease Link Frequency Relevance Heat Mastocytoma 1 100.00 Injury 66 99.72 INFLAMMATION 98 98.68 Brain Injury 3 93.84 Cv Unclassified Under Development 39 93.52 Stroke 51 93.40 Targeted Disruption 6 87.84 Leukemia 1 87.24 Brain Hemorrhage 24 80.32 Infarction 24 80.08

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Cellular production of 5-lipoxygenase products was inhibited by SK&F 86002 as measured by leukotriene B4 (LTB4) generation from human neutrophils (IC50 20 microM), leukotriene C4 (LTC4) generation by human monocytes (IC50 20 microM), and 5-HETE production by RBL-1 cells (IC50 40 microM).

Negative_regulation (inhibited) of Gene_expression (production) of 5-lipoxygenase in neutrophils

1) Confidence 0.42 Published 1987 Journal Biochem. Pharmacol. Section Abstract Doc Link 2823821 Disease Relevance 0.53 Pain Relevance 0.23

L-656,224 (7-chloro-2-[(4-methoxyphenyl)methyl]-3-methyl-5-propyl-4-benzofuranol) was a potent inhibitor of leukotriene biosynthesis in intact rat and human leukocytes and CXBG mastocytoma cells (IC50 values, 18-240 nM) and of crude human leukocyte and highly purified porcine leukocyte 5-lipoxygenase (IC50 value, 4 X 10(-7) M).

Negative_regulation (inhibitor) of Gene_expression (biosynthesis) of 5-lipoxygenase in leukocyte associated with mastocytoma

2) Confidence 0.41 Published 1987 Journal Can. J. Physiol. Pharmacol. Section Abstract Doc Link 2835137 Disease Relevance 0.25 Pain Relevance 0.13

It is concluded that in astroglial cells derived from cortex of new-born rats products of 5-lipoxygenase can diminish the action of protein kinase C on the proenkephalin gene.

Negative_regulation (diminish) of Gene_expression (products) of 5-lipoxygenase in cortex associated with kinase c

3) Confidence 0.41 Published 1995 Journal Brain Res. Mol. Brain Res. Section Abstract Doc Link 8774948 Disease Relevance 0 Pain Relevance 0.08

Evaluation of 5-LOX metabolites-by LC/MS/MS and ELISA confirmed that both compounds selectively inhibited all products downstream of 5-hydroperoxy eicosatetraenoic acid (5-HPETE), including 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxoETE), without inhibition of 12-lipoxygenase (12-LOX), 15-lipoxygenase (15-LOX), or cyclooxygenase (COX) products.

Negative_regulation (inhibition) of Gene_expression (products) of 5-lipoxygenase

4) Confidence 0.41 Published 2008 Journal Eur. J. Pharmacol. Section Abstract Doc Link 18295198 Disease Relevance 0.05 Pain Relevance 0.07

To investigate the increased expression of the 5-LOX enzyme after IR injury, and whether administration of BW-B 70C reduces the 5-LOX expression, we subjected rat brain tissue sections to immunohistochemistry (Fig. 1E, a–c).

Negative_regulation (reduces) of Gene_expression (expression) of 5-LOX in brain associated with injury

5) Confidence 0.14 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1526713 Disease Relevance 0.61 Pain Relevance 0.11

Thus, inhibiting the 5-LOX/NF-?

Negative_regulation (inhibiting) of Gene_expression (/) of 5-LOX

6) Confidence 0.14 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1526713 Disease Relevance 0.69 Pain Relevance 0.25

Inhibiting the 5-LOX/NF-?

Negative_regulation (Inhibiting) of Gene_expression (/) of 5-LOX

7) Confidence 0.14 Published 2006 Journal J Neuroinflammation Section Body Doc Link PMC1526713 Disease Relevance 0.72 Pain Relevance 0.14

The 3-acetoxy derivative of 3-oxo-TA acted like 3-oxo-TA in intact PMNs, whereas 3-hydroxy-TA barely stimulated MEK phosphorylation in resting cells and showed only inhibition on ionophore-induced 5-LO product synthesis.

Negative_regulation (inhibition) of Gene_expression (synthesis) of 5-LO

8) Confidence 0.03 Published 2001 Journal Mol. Pharmacol. Section Abstract Doc Link 11455013 Disease Relevance 0.05 Pain Relevance 0.05

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