INT292386

Context Info Confidence 0.41 First Reported 2010 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 12 Disease Relevance 0.67 Pain Relevance 0

nucleoplasm (Itpr3) transport (Itpr3) endoplasmic reticulum (Itpr3) nucleolus (Itpr3) nucleus (Itpr3) transmembrane transport (Itpr3)

Anatomy Link Frequency taste buds 1 Itpr3 (Mus musculus) Lrmp (Mus musculus)

Pain Link Frequency Relevance Heat member 8 24 5.00

Disease Link Frequency Relevance Heat Adenoma 12 85.20 Diabetes Mellitus 12 84.88 Adhesions 12 45.16 Hypersensitivity 24 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Because IP3R3 was coexpressed with Trpm5 in taste buds (Pérez et al. 2002), we predicted that Lrmp/Jaw1 may colocalize and interact with IP3R3.

IP3R3 Binding (interact) of Lrmp/Jaw1 in taste buds

1) Confidence 0.41 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

Lrmp/Jaw1 associated with IP3R3 in COS7 heterologous expression system

IP3R3 Binding (associated) of Lrmp/Jaw1

2) Confidence 0.41 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

Further investigation using mice lacking Lrmp/Jaw1 would provide insights regarding the role of Lrmp/Jaw1–IP3R3 interaction in taste tissue in vivo.

IP3R3 Binding (interaction) of Lrmp/Jaw1

3) Confidence 0.30 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0.17 Pain Relevance 0

Further investigation using mice lacking Lrmp/Jaw1 would provide insights regarding the role of Lrmp/Jaw1–IP3R3 interaction in taste tissue in vivo.

IP3R3 Binding (interaction) of Lrmp/Jaw1

4) Confidence 0.30 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0.17 Pain Relevance 0

Furthermore, we showed that Lrmp/Jaw1 may engage in direct protein–protein interaction with IP3R3.

IP3R3 Binding (engage) of Lrmp/Jaw1

5) Confidence 0.30 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

We also showed that Lrmp/Jaw1 may engage in direct protein–protein interaction with IP3R3.

IP3R3 Binding (interaction) of Lrmp/Jaw1

6) Confidence 0.30 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0.17 Pain Relevance 0

Furthermore, we showed that Lrmp/Jaw1 may engage in direct protein–protein interaction with IP3R3.

IP3R3 Binding (interaction) of Lrmp/Jaw1

7) Confidence 0.30 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

These results suggest that Lrmp/Jaw1 is associated with IP3R3 via its coiled-coil domain in the COS7 heterologous expression system.

IP3R3 Binding (associated) of Lrmp/Jaw1

8) Confidence 0.30 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

We also showed that Lrmp/Jaw1 may engage in direct protein–protein interaction with IP3R3.

IP3R3 Binding (engage) of Lrmp/Jaw1

9) Confidence 0.30 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0.17 Pain Relevance 0

These results raise the possibility that Lrmp/Jaw1 interacts with IP3R3 in taste cells and suggest an important role for Lrmp/Jaw1 in the IP3-Ca2+ signal cascade in sweet, bitter, and umami taste signal transduction.

IP3R3 Binding (interacts) of Lrmp/Jaw1

10) Confidence 0.30 Published 2010 Journal Chemical Senses Section Abstract Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

To investigate the protein–protein interaction between Lrmp/Jaw1 and IP3R3, we utilized the COS7 heterologous expression system and the FLAG epitope–tagged Lrmp/Jaw1 construct with/without a coiled-coil domain (FLAG-Lrmp/Jaw1 and FLAG-Lrmp/Jaw1?

IP3R3 Binding (interaction) of Lrmp/Jaw1

11) Confidence 0.30 Published 2010 Journal Chemical Senses Section Body Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

In addition to this specific expression patterns, we found that Lrmp/Jaw1 is associated with type III IP3 receptor (IP3R3) via its coiled-coil domain in the COS7 heterologous expression system.

IP3 Binding (associated) of Lrmp/Jaw1

12) Confidence 0.26 Published 2010 Journal Chemical Senses Section Abstract Doc Link PMC2805811 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.