INT292921

Context Info Confidence 0.08 First Reported 2010 Last Reported 2010 Negated 1 Speculated 4 Reported most in Body Documents 1 Total Number 5 Disease Relevance 0 Pain Relevance 1.35

nuclear envelope (Mns1) nucleus (Mns1)

Anatomy Link Frequency synapse 1 Mns1 (Rattus norvegicus)

Pain Link Frequency Relevance Heat tetrodotoxin 45 98.48 addiction 5 93.08 Glutamate 40 87.68 Action potential 10 66.32 opiate 80 64.96 gABA 5 64.56 opioid receptor 75 44.72 antagonist 10 27.04 Opioid 75 18.40 depression 130 17.12

Disease Link Frequency Relevance Heat Depression 115 17.12 Respiratory Failure 15 5.00 Sleep Disorders 10 5.00 Apnoea 10 5.00 Central Sleep Apnea 5 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

The effect of drug on inspiratory synaptic currents in XII MNs was also examined.

Spec (examined) Regulation (effect) of MNs

1) Confidence 0.08 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808240 Disease Relevance 0 Pain Relevance 0.18

The effect of CX717 on mini EPSCs in XII inspiratory MNs was also examined in the presence of TTX (1 µM), bicuculline (10 µM), strychnine (10 µM) and DAMGO (500 nM).

Spec (examined) Regulation (effect) of MNs associated with tetrodotoxin

2) Confidence 0.08 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808240 Disease Relevance 0 Pain Relevance 0.35

We also examined the effect of CX717 on AMPA receptor currents in XII MNs.

Spec (examined) Regulation (effect) of MNs

3) Confidence 0.08 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808240 Disease Relevance 0 Pain Relevance 0.37

Given that DAMGO had no noticeable effect on the membrane properties of XII MNs, we next assessed whether the DAMGO-evoked reduction in inspiratory burst amplitude reflected an action on glutamatergic synaptic transmission at the pre-motor to XII MN synapse.

Neg (no) Regulation (effect) of MNs in synapse

4) Confidence 0.08 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808240 Disease Relevance 0 Pain Relevance 0.14

To further investigate the mechanism underlying the CX614-mediated enhancement of inspiratory burst activity, we examined the effect of CX614 on mini EPSCs in XII inspiratory MNs.

Spec (examined) Regulation (effect) of MNs

5) Confidence 0.08 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808240 Disease Relevance 0 Pain Relevance 0.32

General Comments

This test has worked.