INT292953

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Context Info
Confidence 0.78
First Reported 2010
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 52
Disease Relevance 30.08
Pain Relevance 0.05

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleus (Fto) molecular_function (Fto) cellular_component (Fto)
Anatomy Link Frequency
neuronal 6
fat 5
ARC 3
hypothalamus 2
brain 1
Fto (Rattus norvegicus)
Pain Link Frequency Relevance Heat
anesthesia 52 51.64 Quite High
ketamine 52 49.04 Quite Low
Disease Link Frequency Relevance Heat
Aids-related Complex 2340 99.98 Very High Very High Very High
Body Weight 260 99.54 Very High Very High Very High
Targeted Disruption 52 99.00 Very High Very High Very High
Apoptosis 52 97.04 Very High Very High Very High
Appetite Loss 52 96.60 Very High Very High Very High
Hyperphagia 52 96.48 Very High Very High Very High
Obesity 572 95.52 Very High Very High Very High
Cytomegalovirus Infection 52 92.80 High High
Viral Infection 52 24.84 Low Low
Peripheral Arterial Disease 52 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
However, it is noteworthy that reduction in Fto expression in only a limited number of ARC cells is sufficient to affect food intake.
Gene_expression (expression) of Fto associated with aids-related complex
1) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.79 Pain Relevance 0
Over-expression of Fto in either ARC or PVN caused a reciprocal reduction in food intake.
Gene_expression (expression) of Fto associated with aids-related complex
2) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.81 Pain Relevance 0
In contrast, knocking down Arc Fto expression by 40% increases food intake by 16%. mRNA levels of Agrp, Pomc and Npy, ARC-expressed genes classically associated with the control of food intake, were not affected by the manipulation of Fto expression.
Gene_expression (expression) of Fto in ARC associated with aids-related complex
3) Confidence 0.78 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2808248 Disease Relevance 0.66 Pain Relevance 0
The rat Fto specific shRNA that we generated effectively inhibited the expression of endogenous Fto in hypothalamic GT1-7 cells by 90%, whereas transfection with scrambled control did not affect the production of the Fto protein (Fig 2A).
Gene_expression (production) of Fto
4) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.35 Pain Relevance 0
To modulate Fto expression levels in the ARC and PVN, we generated four AAV 2/7vectors: i.)
Gene_expression (expression) of Fto associated with aids-related complex
5) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.48 Pain Relevance 0
Three animals (2 from the ARC study and 1 from the PVN study) were excluded from further analysis as expression of both Fto RNA and protein levels in these animals were not altered at least 5% as compared to controls.
Gene_expression (expression) of Fto associated with aids-related complex
6) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.56 Pain Relevance 0
In contrast, knocking down Arc Fto expression by 40% increases food intake by 16%. mRNA levels of Agrp, Pomc and Npy, ARC-expressed genes classically associated with the control of food intake, were not affected by the manipulation of Fto expression.
Gene_expression (expression) of Fto in ARC associated with aids-related complex
7) Confidence 0.78 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2808248 Disease Relevance 0.61 Pain Relevance 0
However, over expression of Fto resulted in a 4-fold increase in the mRNA levels of Stat3, suggesting a possible candidate for the mediation of Fto's actions within the ARC.
Gene_expression (expression) of Fto associated with aids-related complex
8) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.81 Pain Relevance 0
For the over expression studies, at the protein level, we achieved approximately 2.5 fold increase in total Fto levels in the ARC and a 1.5 fold increase in the PVN.
Gene_expression (levels) of Fto associated with aids-related complex
9) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.55 Pain Relevance 0
The increase in Stat3 expression associated with overexpression of Fto and reduced food intake is consistent with its potential role in mediating leptin's anorectic effects.
Gene_expression (overexpression) of Fto associated with appetite loss
10) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.68 Pain Relevance 0
However, over expression of Fto resulted in a 4-fold increase in the mRNA levels of Stat3, a signalling molecule critical for leptin receptor signalling, suggesting a possible candidate for the mediation of Fto's actions.
Gene_expression (expression) of Fto
11) Confidence 0.78 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2808248 Disease Relevance 0.60 Pain Relevance 0
Fasting reduces levels of Fto mRNA in the arcuate nucleus (ARC) of the hypothalamus, a site where Fto expression is particularly high.
Gene_expression (expression) of Fto in hypothalamus associated with aids-related complex
12) Confidence 0.78 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2808248 Disease Relevance 0.56 Pain Relevance 0
Conversely, a 40% reduction in Fto protein expression resulted in an increase in food intake (Fig 3C, D).
Gene_expression (expression) of Fto
13) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.52 Pain Relevance 0
To assess the specificity of AAV delivery, at the end of the study, 3 weeks after AAV injections, micro-punches from both the ARC or PVN were obtained for all experimental animal and tested for changes in Fto expression using real-time quantitative RT-PCR (Fig 2B).
Gene_expression (expression) of Fto associated with aids-related complex
14) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.45 Pain Relevance 0
For double-labelling of tissue sections with both isotopic and non-isotopic cRNA probes, a modification of our previously described protocol to detect Fto expression [16] was employed.
Gene_expression (expression) of Fto
15) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.19 Pain Relevance 0.05
We have further explored the role of hypothalamic Fto in the control of food intake by using stereotactic injections coupled with AAV technology to bi-directionally modulate Fto expression.
Gene_expression (expression) of Fto
16) Confidence 0.78 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2808248 Disease Relevance 0.66 Pain Relevance 0
Over expression of Fto in the ARC resulted in a decrease in food intake, compared to rats injected with empty vector (Fig 3A, B).
Gene_expression (expression) of Fto associated with aids-related complex
17) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.60 Pain Relevance 0
At the end of the experiment, we check for Fto expression as an indication for correct site of injection.
Gene_expression (expression) of Fto
18) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.58 Pain Relevance 0
Whilst for the knockdown experiments, Fto expression was decreased by 40% in both the ARC and the PVN compared to rats injected with scrambled AAV.


Gene_expression (expression) of Fto associated with aids-related complex
19) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.56 Pain Relevance 0
As a comparator, we also perturbed expression of Fto in the PVN.
Gene_expression (expression) of Fto
20) Confidence 0.78 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808248 Disease Relevance 0.42 Pain Relevance 0

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