INT295162
From wiki-pain
|
|
|
|
|
Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Either of these outcomes could perturb the overall regulation of mdr1 mRNA and protein expression. | |||||||||||||||
| |||||||||||||||
|
In order to determine whether the increase in genomic copies and transcription of the mdr1 gene was accompanied by an increase in its product, we measured the relative amount of MDR1 protein in the different parasite clones, using quantitative Western blot analysis. | |||||||||||||||
| |||||||||||||||
|
Thus, the fold-increase of AS-ATNMF1 mdr1 mRNA transcripts was 2.6±0.17 and 3.4±0.22, relative to its single copy number progenitor AS-ATN and to AS-3CQ, respectively. | |||||||||||||||
| |||||||||||||||
|
The biodistribution of human MDR1 by in situ hybridization was consistent with the expression of P-gp. | |||||||||||||||
| |||||||||||||||
|
Detection of human MDR1 gene biodistribution | |||||||||||||||
| |||||||||||||||
|
In this context, we investigated by real time PCR whether the evolution of ATN + MF-resistance had selected parasites with increased mdr1 copy number or mRNA expression. | |||||||||||||||
| |||||||||||||||
|
Investigation of candidate genes revealed an mdr1 duplication in the resistant parasites and increased levels of mdr1 transcripts and protein. | |||||||||||||||
| |||||||||||||||
|
(Figure 1) MDR1 mRNA highly expressed in the treated BMCs which showed a unique MDR1 specific band compared with the untreated cells. | |||||||||||||||
| |||||||||||||||
|
General Comments
This test has worked.