INT295162

Context Info Confidence 0.59 First Reported 2010 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 4 Total Number 8 Disease Relevance 1.69 Pain Relevance 0.11

transport (Abcb1b) mitochondrion (Abcb1b) ATPase activity (Abcb1b) transmembrane transport (Abcb1b)

Anatomy Link Frequency band 1 Abcb1b (Mus musculus)

Pain Link Frequency Relevance Heat alcohol 3 79.52 tolerance 8 77.36 vincristine 6 73.00 anesthesia 11 31.36 cytokine 6 5.00 imagery 5 5.00 Central nervous system 3 5.00 Inflammatory response 3 5.00

Disease Link Frequency Relevance Heat Colon Cancer 21 98.88 Toxicity 3 88.04 Body Weight 3 88.04 Infection 29 86.16 Cancer 76 83.44 Injury 3 81.72 Malaria 40 75.20 Falciparum Malaria 5 62.56 Leukemoid Reaction 3 35.76 Recurrence 5 31.60

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Either of these outcomes could perturb the overall regulation of mdr1 mRNA and protein expression.

Transcription (expression) of mdr1

1) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2904698 Disease Relevance 0.14 Pain Relevance 0

In order to determine whether the increase in genomic copies and transcription of the mdr1 gene was accompanied by an increase in its product, we measured the relative amount of MDR1 protein in the different parasite clones, using quantitative Western blot analysis.

Transcription (transcription) of mdr1

2) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2904698 Disease Relevance 0 Pain Relevance 0

Thus, the fold-increase of AS-ATNMF1 mdr1 mRNA transcripts was 2.6±0.17 and 3.4±0.22, relative to its single copy number progenitor AS-ATN and to AS-3CQ, respectively.

Transcription (transcripts) of mdr1

3) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2904698 Disease Relevance 0 Pain Relevance 0

The biodistribution of human MDR1 by in situ hybridization was consistent with the expression of P-gp.

Transcription (biodistribution) of MDR1

4) Confidence 0.51 Published 2010 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2819043 Disease Relevance 0.33 Pain Relevance 0.04

Detection of human MDR1 gene biodistribution

Transcription (biodistribution) of MDR1

5) Confidence 0.51 Published 2010 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2819043 Disease Relevance 0.75 Pain Relevance 0.04

In this context, we investigated by real time PCR whether the evolution of ATN + MF-resistance had selected parasites with increased mdr1 copy number or mRNA expression.

Transcription (expression) of mdr1

6) Confidence 0.44 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2904698 Disease Relevance 0 Pain Relevance 0.04

Investigation of candidate genes revealed an mdr1 duplication in the resistant parasites and increased levels of mdr1 transcripts and protein.

Transcription (transcripts) of mdr1

7) Confidence 0.44 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2904698 Disease Relevance 0.21 Pain Relevance 0

(Figure 1) MDR1 mRNA highly expressed in the treated BMCs which showed a unique MDR1 specific band compared with the untreated cells.

Transcription (expressed) of MDR1 in band

8) Confidence 0.34 Published 2010 Journal J Exp Clin Cancer Res Section Body Doc Link PMC2819043 Disease Relevance 0.26 Pain Relevance 0

General Comments

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