INT298235

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Context Info
Confidence 0.47
First Reported 2010
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 14
Disease Relevance 2.95
Pain Relevance 1.11

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleus (Pou4f1) DNA binding (Pou4f1)
Anatomy Link Frequency
embryo 1
Pou4f1 (Mus musculus)
Pain Link Frequency Relevance Heat
trigeminal ganglion 1050 98.64 Very High Very High Very High
nociceptor 448 85.08 High High
mu opioid receptor 56 45.48 Quite Low
calcitonin gene related peptide 154 41.40 Quite Low
Neuropeptide 56 36.44 Quite Low
substance P 42 5.00 Very Low Very Low Very Low
Somatostatin 42 5.00 Very Low Very Low Very Low
Central nervous system 28 5.00 Very Low Very Low Very Low
Spinal cord 28 5.00 Very Low Very Low Very Low
Serotonin 28 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Targeted Disruption 602 100.00 Very High Very High Very High
Ganglion Cysts 1190 98.64 Very High Very High Very High
Repression 98 55.60 Quite High
Pain 42 5.00 Very Low Very Low Very Low
Nociception 42 5.00 Very Low Very Low Very Low
Neurodegenerative Disease 42 5.00 Very Low Very Low Very Low
Death 28 5.00 Very Low Very Low Very Low
Herpes Simplex Virus 14 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Brn3a binding to the identified sites was tested in vitro using EMSA with oligonucleotide probes and cell lysates from Brn3a-transfected HEK293 cells.
Brn3a Binding (binding) of
1) Confidence 0.47 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.14 Pain Relevance 0.07
Because we have previously shown that in vitro binding of Brn3a to a specific regulatory element is a necessary but not sufficient requirement for in vivo site occupancy [14], we next used ChIP to assay Brn3a binding to its Runx3 locus recognition sites in the TG.
Brn3a Binding (binding) of associated with trigeminal ganglion
2) Confidence 0.47 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.16 Pain Relevance 0.08
Brn3a-VP16 was expressed under control of an 11-kb Brn3a sensory enhancer/promoter, in which the native sequences mediating Brn3a binding have been mutated to eliminate negative autoregulation [21].


Brn3a Binding (binding) of
3) Confidence 0.47 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.09 Pain Relevance 0
Here we have shown that Brn3a interacts directly in vivo with an enhancer element located approximately 94 kb upstream of the Runx3 transcriptional start site.
Brn3a Binding (interacts) of
4) Confidence 0.47 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.38 Pain Relevance 0.15
Notably, a site at -25 kb, which bound Brn3a efficiently in vitro, did not appear to be occupied in vivo.
Brn3a Binding (bound) of
5) Confidence 0.47 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.05 Pain Relevance 0.03
For analysis of VP16-Brn3a transgenic ganglia, RNA was prepared from a pair of TG from a single E13.5 embryo, yielding 0.5 to 1.0 ?
VP16-Brn3a Binding (ganglia) of in embryo associated with targeted disruption and trigeminal ganglion
6) Confidence 0.36 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.77 Pain Relevance 0.17
ChIP assays provide less consistent evidence for Brn3a binding to two other regions of the Runx3 locus, and other sites may exist that were not predicted by the consensus recognition sequence used and thus were not tested in this study.


Brn3a Binding (binding) of
7) Confidence 0.35 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.17 Pain Relevance 0.06
In prior work we have demonstrated that binding of Brn3a autoregulatory regions in the Pou4f1 locus and to downstream target gene loci is highly correlated with H3 acetylation [14].
Brn3a Binding (binding) of
8) Confidence 0.35 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.16 Pain Relevance 0.05
The majority of sites tested gave ChIP enrichment values of two-fold or less relative to the Alb locus, indicating that these sites are probably not occupied by Brn3a in vivo.
Brn3a Binding (occupied) of
9) Confidence 0.35 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.06 Pain Relevance 0.03
Brn3a has been shown to have an optimal binding site of ATAATTAAT but also recognizes sequences with conservative substitutions conforming either to the consensus ATA/TAA/TTA/TAT or having a single base difference from the optimal site at specific positions [29].
Brn3a Binding (recognizes) of
10) Confidence 0.35 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.13 Pain Relevance 0.04
In prior work we have demonstrated that binding of Brn3a autoregulatory regions in the Pou4f1 locus and to downstream target gene loci is highly correlated with H3 acetylation [14].
Pou4f1 Binding (binding) of
11) Confidence 0.35 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.17 Pain Relevance 0.06
Chromatin immunoprecipitation confirms that Brn3a binds in vivo to a conserved upstream enhancer element within histone H3-acetylated chromatin in the Runx3 locus.
Brn3a Binding (binds) of
12) Confidence 0.35 Published 2010 Journal Neural Dev Section Abstract Doc Link PMC2829025 Disease Relevance 0.49 Pain Relevance 0.30
Because we have previously shown that in vitro binding of Brn3a to a specific regulatory element is a necessary but not sufficient requirement for in vivo site occupancy [14], we next used ChIP to assay Brn3a binding to its Runx3 locus recognition sites in the TG.
Brn3a Binding (binding) of associated with trigeminal ganglion
13) Confidence 0.32 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0.16 Pain Relevance 0.08
In addition, the region contains multiple TAAT motifs that may accommodate multimeric Brn3a binding or transcriptional partners.
Brn3a Binding (binding) of
14) Confidence 0.31 Published 2010 Journal Neural Dev Section Body Doc Link PMC2829025 Disease Relevance 0 Pain Relevance 0

General Comments

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