INT301208

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Context Info
Confidence 0.17
First Reported 2010
Last Reported 2010
Negated 0
Speculated 1
Reported most in Body
Documents 1
Total Number 51
Disease Relevance 26.18
Pain Relevance 0

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
granulocytes 3
hematopoietic system 2
myeloid progenitor cells 1
megakaryocyte 1
hematopoietic cells 1
Mir29a (Mus musculus)
Pain Link Frequency Relevance Heat
isoflurane 51 11.28 Low Low
anesthesia 51 9.52 Low Low
Disease Link Frequency Relevance Heat
Myeloproliferative Disorder 1122 99.96 Very High Very High Very High
Leukemia 867 99.96 Very High Very High Very High
Myeloid Leukemia 2856 99.76 Very High Very High Very High
Starvation 51 99.48 Very High Very High Very High
Apoptosis 153 99.20 Very High Very High Very High
Cancer 357 99.04 Very High Very High Very High
Disease 408 98.60 Very High Very High Very High
Disease Progression 51 98.44 Very High Very High Very High
Targeted Disruption 102 98.00 Very High Very High Very High
Hematological Disease 306 97.68 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
It is therefore conceivable that the clonal expansion may already be established by the time the MPD stage of disease is established, presumably by a few clones with the highest level of miR-29a expression.
Gene_expression (expression) of miR-29a associated with myeloproliferative disorder and disease
1) Confidence 0.17 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.74 Pain Relevance 0
Overexpression of miR-29a in hematopoietic progenitors leads to biased myelopoiesis and an MPD
Gene_expression (Overexpression) of miR-29a associated with myeloproliferative disorder
2) Confidence 0.17 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.10 Pain Relevance 0
Although shRNA knock-down of HBP1 in mouse progenitors did not recapitulate the myeloid proliferation or AML induced by miR-29 overexpression, a coordinating role of HBP1 with other factors such as Slfn4, Dnmt3L, and Cdc42ep2 in leukemic development cannot be excluded.
Gene_expression (overexpression) of miR-29 associated with targeted disruption and myeloid leukemia
3) Confidence 0.17 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.60 Pain Relevance 0
There was no apparent effect of miR-29a overexpression on basal levels of apoptosis or apoptosis induced by serum starvation, suggesting that ectopic expression of miR-29a does not exhibit a significant impact on cell survival (unpublished data).


Gene_expression (overexpression) of miR-29a associated with starvation and apoptosis
4) Confidence 0.17 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.41 Pain Relevance 0
Nevertheless, because self-renewal is normally a unique feature of HSC and high levels of miR-29a expression are associated with HSC and AML, we hypothesize that miR-29a contributes to HSC and LSC self-renewal.
Gene_expression (expression) of miR-29a associated with myeloid leukemia
5) Confidence 0.17 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.71 Pain Relevance 0
Overexpression of miR-29a enhances proliferation of MPP and 293T cells but does not significantly alter MPP self-renewal, yet overexpression of miR-29a in CMP or GMP is sufficient to induce self-renewal but not proliferation, suggesting that miR-29a exerts stage-specific and cell context–specific effects.
Gene_expression (overexpression) of miR-29a
6) Confidence 0.17 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.38 Pain Relevance 0
Overexpression of miR-29a enhances proliferation of MPP and 293T cells but does not significantly alter MPP self-renewal, yet overexpression of miR-29a in CMP or GMP is sufficient to induce self-renewal but not proliferation, suggesting that miR-29a exerts stage-specific and cell context–specific effects.
Gene_expression (Overexpression) of miR-29a
7) Confidence 0.17 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.41 Pain Relevance 0
Expression and appropriate processing of miR-29a was confirmed by RT-PCR and Northern blot analysis (Fig.
Gene_expression (Expression) of miR-29a
8) Confidence 0.17 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.09 Pain Relevance 0
Given the dramatic phenotype induced by miR-29a overexpression, it is likely that miR-29a targets multiple mRNAs in addition to HBP1 to induce the described phenotypes.
Gene_expression (overexpression) of miR-29a
9) Confidence 0.17 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.30 Pain Relevance 0
To determine whether miR-29a positively regulates cell cycle progression, and given that it is difficult to efficiently infect primary myeloid progenitors with retroviral vector, we compared the G1 to S/G2 phase transition between WT 293T and 293T cells overexpressing miR-29a by in vitro BrdU incorporation assays.
Gene_expression (overexpressing) of miR-29a
10) Confidence 0.17 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.45 Pain Relevance 0
Consistently, the proliferation rate of miR-29a–overexpressing 293T cells was significantly enhanced when compared with WT cells (Fig. 5 c).
Gene_expression (overexpressing) of miR-29a
11) Confidence 0.15 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.39 Pain Relevance 0
Mature miR-29a expression was measured using the mirVana qRT-PCR miRNA Detection kit (Ambion).
Gene_expression (expression) of miR-29a
12) Confidence 0.15 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.07 Pain Relevance 0
miR-29a–overexpressing myeloid progenitors are functional LSCs
Gene_expression (overexpressing) of miR-29a
13) Confidence 0.15 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.81 Pain Relevance 0
However, further experiments will be required to completely exclude other possible explanations for the long-term engraftment of miR-29a–expressing myeloid progenitors, including that long-term engrafted miR-29a–overexpressing CMP and GMP represent the progeny of rare engrafted HSC exhibiting a strongly biased myeloid differentiation potential, and that the transplanted and/or engrafted immunophenotypic myeloid progenitors may represent abnormal HSC with altered surface marker expression.
Gene_expression (overexpressing) of miR-29a
14) Confidence 0.15 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.68 Pain Relevance 0
For BrdU incorporation assays, 10 µM BrdU was added to equal numbers of WT 293T cells and 293T cells stably expressing miR-29a cells (in duplicate for each time point).
Gene_expression (expressing) of miR-29a
15) Confidence 0.15 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.06 Pain Relevance 0
Enforced miR-29a expression leads to a high incidence of AML
Gene_expression (expression) of miR-29a associated with myeloid leukemia
16) Confidence 0.15 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.46 Pain Relevance 0
We found that miR-29a–overexpressing 293T cells entered S/G2 phase more rapidly than WT 293T cells, with ?
Gene_expression (overexpressing) of miR-29a
17) Confidence 0.15 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.46 Pain Relevance 0
However, further experiments will be required to completely exclude other possible explanations for the long-term engraftment of miR-29a–expressing myeloid progenitors, including that long-term engrafted miR-29a–overexpressing CMP and GMP represent the progeny of rare engrafted HSC exhibiting a strongly biased myeloid differentiation potential, and that the transplanted and/or engrafted immunophenotypic myeloid progenitors may represent abnormal HSC with altered surface marker expression.
Gene_expression (expressing) of miR-29a
18) Confidence 0.15 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.75 Pain Relevance 0
Because aberrant acquisition of self-renewal capacity is likely an important step in myeloid leukemogenesis (Passegué et al., 2003), we sought to determine whether miR-29a–overexpressing progenitors acquire this capacity during the early phase of MPD.
Spec (whether) Gene_expression (overexpressing) of miR-29a associated with myeloproliferative disorder
19) Confidence 0.15 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.27 Pain Relevance 0
There was no apparent effect of miR-29a overexpression on basal levels of apoptosis or apoptosis induced by serum starvation, suggesting that ectopic expression of miR-29a does not exhibit a significant impact on cell survival (unpublished data).


Gene_expression (expression) of miR-29a associated with starvation and apoptosis
20) Confidence 0.15 Published 2010 Journal The Journal of Experimental Medicine Section Body Doc Link PMC2839143 Disease Relevance 0.41 Pain Relevance 0

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