INT301442

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Context Info
Confidence 0.77
First Reported 2010
Last Reported 2010
Negated 1
Speculated 1
Reported most in Body
Documents 1
Total Number 36
Disease Relevance 2.97
Pain Relevance 0.17

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytosol (Smyd2) methyltransferase activity (Smyd2) nucleus (Smyd2)
cytoplasm (Smyd2)
Anatomy Link Frequency
heart 15
cardiomyocytes 5
brain 3
heart ventricles 2
cardiac ventricles 1
Smyd2 (Mus musculus)
Pain Link Frequency Relevance Heat
fibrosis 36 75.36 Quite High
imagery 216 5.00 Very Low Very Low Very Low
anesthesia 36 5.00 Very Low Very Low Very Low
isoflurane 36 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Body Weight 36 96.84 Very High Very High Very High
Congenital Anomalies 36 89.44 High High
Embryonic Lethality 72 87.80 High High
Targeted Disruption 252 87.52 High High
Stroke 36 87.20 High High
Cancer 72 86.00 High High
Necrosis 72 79.12 Quite High
Cardiovascular Disorder Under Development 36 75.36 Quite High
Stress 108 67.72 Quite High
Repression 36 66.76 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Since Smyd2 expression has not yet been described in the neonatal heart and previous expression analyses were only performed at the mRNA level using Northern-blotting and in situ hybridization [20], we next investigated Smyd2 protein expression in heart ventricles during cardiac development.
Spec (investigated) Gene_expression (expression) of Smyd2 protein in heart ventricles
1) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0
Based on the observation that Smyd2 expression peaks after birth, we expected maximal changes in target gene expression between Smyd2 cKO and control animals at that time.
Gene_expression (expression) of Smyd2
2) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0.21 Pain Relevance 0.03
As Smyd2 expression in the heart peaks shortly after birth, we re-evaluated Smyd2 expression in other organs at that time as our previous evaluation was performed only in adult mouse organs where Smyd2 expression is almost absent [20].
Gene_expression (expression) of Smyd2 in heart
3) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0
Taken together, the data suggest that Smyd2 does not contribute non-redundantly to development or maintenance of normal cardiac morphology even though neonatal cardiomyocytes are the primary site of Smyd2 expression.


Gene_expression (expression) of Smyd2 in cardiomyocytes
4) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0.29 Pain Relevance 0.04
Given a peak expression of Smyd2 in the first week of postnatal life, one might anticipate that Smyd2 is important for the biological processes occurring during this time period, namely the irreversible exit from cell cycle [50]–[52] as well as the change from mainly lactate and glucose catabolism to mitochondrial fatty acid oxidation [53].
Gene_expression (expression) of Smyd2
5) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0.09 Pain Relevance 0
Since Smyd2 expression is not restricted to the heart (Fig. 1C), we generated mice that allow the tissue specific deletion of Smyd2 expression.
Gene_expression (expression) of Smyd2 in heart
6) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0
Western-Blot analysis showed relatively high Smyd2 protein expression in P1 cardiac ventricles while very low expression was detected at E12.5 and adult.
Gene_expression (expression) of Smyd2 protein in cardiac ventricles
7) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0
As Smyd2 expression in the heart peaks shortly after birth, we re-evaluated Smyd2 expression in other organs at that time as our previous evaluation was performed only in adult mouse organs where Smyd2 expression is almost absent [20].
Neg (absent) Gene_expression (expression) of Smyd2 in heart
8) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0
An alternative and trivial explanation might be that Smyd2 is predominantly expressed in cardiomyocytes which make up only 56% of all cell types in the murine heart [60].
Gene_expression (expressed) of Smyd2 in heart
9) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0.19 Pain Relevance 0
Immunohistochemistry using cryosections from neonatal (P1) mouse hearts further confirmed loss of Smyd2 expression in cKO animals (Fig. 4D).
Gene_expression (expression) of Smyd2 in hearts
10) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0.15 Pain Relevance 0
Smyd2 is specifically expressed in cardiomyocytes
Gene_expression (expressed) of Smyd2 in cardiomyocytes
11) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0
Since Smyd2 expression is not restricted to the heart (Fig. 1C), we generated mice that allow the tissue specific deletion of Smyd2 expression.
Gene_expression (expression) of Smyd2 in heart
12) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0
Since Smyd2 expression has not yet been described in the neonatal heart and previous expression analyses were only performed at the mRNA level using Northern-blotting and in situ hybridization [20], we next investigated Smyd2 protein expression in heart ventricles during cardiac development.
Gene_expression (expression) of Smyd2 in heart ventricles
13) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0
In line with our previous findings, Smyd2 showed a broader organ distribution pattern compared to Smyd1 but was most highly expressed in heart, skeletal muscle and brain tissue.
Gene_expression (expressed) of Smyd2 in brain
14) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0
As Smyd2 expression in the heart peaks shortly after birth, we re-evaluated Smyd2 expression in other organs at that time as our previous evaluation was performed only in adult mouse organs where Smyd2 expression is almost absent [20].
Gene_expression (expression) of Smyd2 in heart
15) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0
This analysis confirmed the significant knockdown of Smyd2 expression and revealed that Smyd1/mBop expression was modestly, but statistically significantly, elevated (1.3-fold±0.09) in the cKO animals.
Gene_expression (expression) of Smyd2
16) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0.12 Pain Relevance 0
Since Smyd2 is most highly expressed after birth, we also examined the hearts of neonatal (P3) as well as adult (>8 weeks) mice by histological analysis using hematoxylin and eosin staining as well as Masson's trichrome staining.
Gene_expression (expressed) of Smyd2 in hearts
17) Confidence 0.77 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0.25 Pain Relevance 0.04
The Smyd2 cKO hearts appeared normal (Fig. 5A), and we detected no significant changes in heart to body weight ratios compared to control mice at 6 month of age (Fig. 5B).
Gene_expression (hearts) of Smyd2 cKO in body associated with body weight
18) Confidence 0.67 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0.21 Pain Relevance 0.03
Molecular analysis of Smyd2 cKO hearts
Gene_expression (hearts) of Smyd2 cKO in hearts
19) Confidence 0.67 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0.28 Pain Relevance 0.04
Generation of mice containing floxed Smyd2 alleles
Gene_expression (containing) of Smyd2
20) Confidence 0.67 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2840034 Disease Relevance 0 Pain Relevance 0

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