INT303211

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Context Info
Confidence 0.64
First Reported 2010
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 13
Disease Relevance 0.48
Pain Relevance 0.40

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (Shank1) plasma membrane (Shank1) intracellular (Shank1)
protein complex assembly (Shank1) cytoplasm (Shank1)
Anatomy Link Frequency
neurons 4
dendrites 1
Shank1 (Rattus norvegicus)
Pain Link Frequency Relevance Heat
tetrodotoxin 52 97.12 Very High Very High Very High
Glutamate receptor 26 5.00 Very Low Very Low Very Low
imagery 13 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Attention Deficit Hyperactivity Disorder 13 97.32 Very High Very High Very High
Cognitive Disorder 13 81.32 Quite High
Autism 13 75.52 Quite High
Anxiety Disorder 13 69.88 Quite High
Syndrome 26 5.00 Very Low Very Low Very Low
Intellectual Impairment 13 5.00 Very Low Very Low Very Low
Disease 13 5.00 Very Low Very Low Very Low
Angelman Syndrome 13 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
We next examined whether TRIM3 functions in neurons to regulate expression of endogenous GKAP protein.
Gene_expression (expression) of GKAP protein in neurons
1) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
Although TRIM3 overexpression by itself inhibited GKAP protein expression, it did not prevent an increase of GKAP expression induced by TTX.
Gene_expression (expression) of GKAP protein
2) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0.10 Pain Relevance 0.08
RBCC, and RING still resulted in loss of Shank1A protein in COS-7 cells, even though these mutants should be defective as E3 ligases and were incapable of suppressing GKAP protein expression (Figure 3D).
Gene_expression (expression) of GKAP protein
3) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
In neurons expressing TRIM3/264 shRNA, bicuculline treatment failed to lower GKAP protein expression, despite a higher “baseline” level of GKAP (Figure 4D, E).
Gene_expression (expression) of GKAP protein in neurons
4) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0.08 Pain Relevance 0.06
TRIM3 regulates GKAP and Shank1 expression in hippocampal neurons
Gene_expression (expression) of Shank1 in neurons
5) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
-gal alone, suppressing activity with tetrodotoxin (TTX, 3 µM, 24 h) resulted in higher levels of GKAP staining in dendrites, whereas stimulating activity with bicuculline (50 µM, 24 h) reduced GKAP protein expression (Figure 4D, quantified in E).
Gene_expression (expression) of GKAP protein in dendrites associated with tetrodotoxin
6) Confidence 0.64 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0.07 Pain Relevance 0.10
At least in heterologous cells, therefore, the inhibition of Shank1A protein levels by TRIM3 appears to be unrelated to the E3 ligase activity of TRIM3.
Gene_expression (levels) of Shank1A protein
7) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
The following antibodies were obtained from commercial sources: mouse anti-BERP/TRIM3 (BD Biosciences); rabbit anti-Shank1A (Chemicon); rabbit (ICN), mouse (Promega) anti-?
Gene_expression (/) of Shank1A
8) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0.08
The following antibodies were obtained from commercial sources: mouse anti-BERP/TRIM3 (BD Biosciences); rabbit anti-Shank1A (Chemicon); rabbit (ICN), mouse (Promega) anti-?
Gene_expression (anti) of Shank1A
9) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0.09
Thus, in heterologous cells, TRIM3 specifically suppresses protein expression of GKAP and Shank1A, two PSD scaffolds that directly interact with each other.
Gene_expression (expression) of Shank1A
10) Confidence 0.50 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
These data strongly support the idea that TRIM3 suppresses GKAP protein expression by stimulating UPS-mediated degradation of GKAP.
Gene_expression (expression) of GKAP protein
11) Confidence 0.50 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
This fact notwithstanding, our data are more consistent with the idea that GKAP is the direct target of TRIM3 and that the effect of TRIM3 on Shank1 expression occurs secondarily to GKAP loss.
Gene_expression (expression) of Shank1
12) Confidence 0.50 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
The GFP-Shank1A plasmid was cotransfected into primary rat hippocampal neurons (DIV 14) with pools of RNAi plasmids at a DNA mass ratio of 1?
Gene_expression (cotransfected) of GFP-Shank1A in neurons
13) Confidence 0.44 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0.23 Pain Relevance 0

General Comments

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