INT303239

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Context Info
Confidence 0.76
First Reported 2010
Last Reported 2010
Negated 2
Speculated 0
Reported most in Body
Documents 1
Total Number 50
Disease Relevance 0.77
Pain Relevance 0.93

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

intracellular (Trim3) cytoplasm (Trim3)
Anatomy Link Frequency
neurons 8
spine 5
brain 4
heads 4
band 2
Trim3 (Rattus norvegicus)
Trim3 - C25S (1)
Pain Link Frequency Relevance Heat
tetrodotoxin 200 97.68 Very High Very High Very High
Glutamate receptor 100 87.08 High High
imagery 50 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Attention Deficit Hyperactivity Disorder 50 98.96 Very High Very High Very High
Angelman Syndrome 50 93.24 High High
Syndrome 100 92.24 High High
Intellectual Impairment 50 91.88 High High
Disease 50 55.24 Quite High
Anxiety Disorder 50 20.56 Low Low
Cognitive Disorder 50 11.72 Low Low
Autism 50 9.44 Low Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Indeed, increased activity stimulates TRIM3 mRNA expression in the brain [27].
Gene_expression (expression) of TRIM3 mRNA in brain
1) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
The intensity of endogenous Shank1 immunostaining also showed bidirectional changes with TRIM3 overexpression and TRIM3 RNAi, similar to GKAP (Figure 4B, C).
Gene_expression (overexpression) of TRIM3
2) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0.06
In neurons overexpressing TRIM3, levels of dendritic GKAP were significantly reduced (Figure 4A, quantified in C).
Gene_expression (overexpressing) of TRIM3 in neurons
3) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
By immunoblotting, TRIM3 was expressed in the brain at low levels at embryonic day 15 (E15) and then increased during the first two postnatal weeks before decreasing through adulthood (Figure 2A, B).
Gene_expression (expressed) of TRIM3 in brain
4) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
In addition, proteasomal inhibition by MG-132 did not fully prevent the decrease in Shank1A induced by cotransfection of TRIM3 (data not shown).
Gene_expression (cotransfection) of TRIM3
5) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
In cells expressing wildtype TRIM3, there was a marked increase in the amount of myc-ubiquitinated GKAP, compared to cells lacking TRIM3 or expressing the C22S/C25S mutant.
Gene_expression (expressing) of TRIM3
6) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
However, we observed no major changes in TRIM3 protein expression or localization in dissociated hippocampal cultures in response to chronic TTX or bicuculline.
Gene_expression (expression) of TRIM3
7) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
The morphological changes are presumably a postsynaptic effect, as they are observed in the spines of transfected cells in which TRIM3 expression has been altered.
Gene_expression (expression) of TRIM3 in spines
8) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
When FLAG-tagged TRIM3 was coexpressed with TRIM3/2756 shRNA (FLAG-TRIM3 plasmid does not contain the 3?
Gene_expression (coexpressed) of TRIM3
9) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
In cells overexpressing wildtype TRIM3, endogenous GKAP staining was depleted and treatment with bicuculline had no additional lowering effect.
Gene_expression (overexpressing) of TRIM3
10) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0.09 Pain Relevance 0.09
We did not detect any significant change in TRIM3 expression with treatment times varying from 2 to 24 hours (Figure S2A).
Gene_expression (expression) of TRIM3
11) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0.10
Previous studies showed that TRIM3 mRNA expression is highest in the brain, moderate in the lung and low in liver, kidney, and heart [13].
Gene_expression (expression) of TRIM3 mRNA in brain
12) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
When FLAG-tagged TRIM3 was coexpressed with TRIM3/2756 shRNA (FLAG-TRIM3 plasmid does not contain the 3?
Gene_expression (coexpressed) of TRIM3
13) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
As both bands were similarly detected when the full-length TRIM3 cDNA was overexpressed in COS-7 cells (Figure S1A), the lower band is more likely to be a cleavage product of TRIM3 rather than an alternative spliced form or related protein.
Gene_expression (overexpressed) of TRIM3 in band
14) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
The following antibodies were obtained from commercial sources: mouse anti-BERP/TRIM3 (BD Biosciences); rabbit anti-Shank1A (Chemicon); rabbit (ICN), mouse (Promega) anti-?
Gene_expression (/) of TRIM3
15) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0.08
Subcellular fractionation of rat forebrain showed TRIM3 to be particularly abundant in the light membrane fraction (P3), and sparse in the cytosol (S3) (Figure 2C).
Gene_expression (abundant) of TRIM3 in forebrain
16) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
In cells expressing wildtype TRIM3, there was a marked increase in the amount of myc-ubiquitinated GKAP, compared to cells lacking TRIM3 or expressing the C22S/C25S mutant.
Neg (lacking) Gene_expression (lacking) of TRIM3
17) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
When FLAG-tagged TRIM3 was coexpressed with TRIM3/2756 shRNA (FLAG-TRIM3 plasmid does not contain the 3?
Gene_expression (coexpressed) of TRIM3
18) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0
The following antibodies were obtained from commercial sources: mouse anti-BERP/TRIM3 (BD Biosciences); rabbit anti-Shank1A (Chemicon); rabbit (ICN), mouse (Promega) anti-?
Gene_expression (anti) of TRIM3
19) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0 Pain Relevance 0.09
Although TRIM3 overexpression by itself inhibited GKAP protein expression, it did not prevent an increase of GKAP expression induced by TTX.
Gene_expression (overexpression) of TRIM3
20) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844417 Disease Relevance 0.10 Pain Relevance 0.08

General Comments

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