INT303277

Context Info Confidence 0.94 First Reported 2010 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 3 Total Number 4 Disease Relevance 0.31 Pain Relevance 0

cytosol (Map1lc3a) cytoskeleton (Map1lc3a) cytoplasm (Map1lc3a)

Anatomy Link Frequency face 2 Map1lc3a (Mus musculus)

Pain Link Frequency Relevance Heat medulla 6 5.28 Clonidine 9 5.00 Spinal cord 7 5.00 Hippocampus 5 5.00 midbrain 2 5.00 Substantia nigra 2 5.00 antagonist 2 5.00 carbamazepine 2 5.00 withdrawal 2 5.00 agonist 2 5.00

Disease Link Frequency Relevance Heat Injury 4 72.08 Death 18 65.72 Disease 93 58.64 Motor Neuron Diseases 70 50.88 Toxicity 21 50.00 Apoptosis 14 45.64 Neuroblastoma 1 39.60 Dementia 18 37.48 Weight Loss 4 20.56 Tremor 11 20.04

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

This also allowed us to demonstrate that this increase in LC3-II levels results from an increase in synthesis rather than a decrease in degradation of autophagosomes, as increased levels were also seen in the presence of bafilomycin A1, an autophagy inhibitor that blocks autophagosome-lysosome fusion and therefore LC3-II degradation (27) (Fig. 1E and F).

Protein_catabolism (degradation) of LC3-II

1) Confidence 0.94 Published 2010 Journal Human Molecular Genetics Section Body Doc Link PMC2865373 Disease Relevance 0.06 Pain Relevance 0

LC3-II is bound to both sides of the membrane, and it remains membrane bound even after fusion with lysosomes, after which LC3-II on the cytosolic face of autophagosomes can be recycled (to LC3-I) by Atg4 [10], while the LC3-II on the inner face of the membrane is degraded.

Protein_catabolism (degraded) of LC3 in face

2) Confidence 0.31 Published 2010 Journal Seminars in Cell & Developmental Biology Section Body Doc Link PMC2938570 Disease Relevance 0 Pain Relevance 0

LC3-II is bound to both sides of the membrane, and it remains membrane bound even after fusion with lysosomes, after which LC3-II on the cytosolic face of autophagosomes can be recycled (to LC3-I) by Atg4 [10], while the LC3-II on the inner face of the membrane is degraded.

Protein_catabolism (degraded) of LC3 in face

3) Confidence 0.23 Published 2010 Journal Seminars in Cell & Developmental Biology Section Body Doc Link PMC2938570 Disease Relevance 0 Pain Relevance 0

Autophagosomes sequester the target organelle/protein and fuse with lysosomes, where the contents – and the LC3-II present on the luminal side of the autophagosome – are degraded [67].

Protein_catabolism (degraded) of LC3

4) Confidence 0.17 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844426 Disease Relevance 0.25 Pain Relevance 0

General Comments

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