In situ hybridization (b, c, e, g, i) and immunohistochemistry (a, d, f, h, j) on formaldehyde fixed paraffin sections. a Early expression of PCI in myocytes of the heart at ED 9.5; b PCI positive cells in choroid plexus (arrowheads) in the lateral ventricle of the brain; c skin of upper and lower jaws; d PCI in myocardium at ED 11.5; note absence of staining in non-myocardial cells including the cushion material of the atrioventricular canal and the developing valves in the outflow tract; e, presence of PCI in oesophagus and lung and absence in trachea; f, g pseudoglandular stage of lung development; note presence of PCI in distal tubules whereas proximal tubules are devoid of staining (asterisks); h, i PCI in cardiac region of stomach where the PCI-positive oesophagus enters (arrow) and in gonad; j PCI expression in hindgut, urethra and skin of anal bay.
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