INT309465

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Context Info
Confidence 0.59
First Reported 2010
Last Reported 2010
Negated 1
Speculated 0
Reported most in Body
Documents 24
Total Number 24
Disease Relevance 5.49
Pain Relevance 0.43

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

lyase activity (Xrcc6) nucleolus (Xrcc6) chromosome (Xrcc6)
nucleus (Xrcc6) helicase activity (Xrcc6) DNA binding (Xrcc6)
Anatomy Link Frequency
neurons 4
embryonic stage 2
striatum 1
nervous system 1
Xrcc6 (Mus musculus)
Pain Link Frequency Relevance Heat
cerebral cortex 138 88.16 High High
Demyelination 10 81.04 Quite High
Hippocampus 23 79.92 Quite High
unmyelinated 7 65.80 Quite High
Sciatic nerve 3 58.08 Quite High
hypoalgesia 4 50.00 Quite Low
dorsal root ganglion 1 29.84 Quite Low
Glutamate 1 6.80 Low Low
imagery 47 5.00 Very Low Very Low Very Low
anesthesia 23 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Targeted Disruption 761 100.00 Very High Very High Very High
Cytomegalovirus Infection 69 99.90 Very High Very High Very High
Apoptosis 141 98.28 Very High Very High Very High
Disease 1152 96.60 Very High Very High Very High
Infection 115 96.08 Very High Very High Very High
Aging 138 91.68 High High
Neurodegenerative Disease 51 88.08 High High
Lifespan 115 82.52 Quite High
Demyelinating Disease 10 81.04 Quite High
Cockayne Syndrome 23 80.40 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
This suggests that, after a short-term transient fluctuation at the embryonic stage, the Ku70 protein expression level becomes stable at the equilibrium between increased synthesis and increased degradation of Ku70.
Gene_expression (expression) of Ku70 protein in embryonic stage
1) Confidence 0.59 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.20 Pain Relevance 0
To support this idea, we found that coexpression of Ku70 substantially elongated survival of Drosophila expressing mutant human Htt (unpublished data).
Gene_expression (coexpression) of Ku70
2) Confidence 0.59 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 1.04 Pain Relevance 0
Mutant Htt induces Ku70 expression
Gene_expression (expression) of Ku70
3) Confidence 0.59 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.06 Pain Relevance 0
We hypothesized that additional overexpression of Ku70 would change the equilibrium between mutant Htt and Ku70 and would increase the amount of free and functional Ku70 (nonbound to mutant Htt).
Gene_expression (overexpression) of Ku70
4) Confidence 0.51 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.16 Pain Relevance 0.06
To generate transgenic mice overexpressing mouse Ku70 in the nervous system, the RIKEN mouse FANTOM clone encoding full-length mouse Ku70 was inserted downstream of the enhancer/promoter of the rat NSE gene (promoter; nucleotides 146,320,892–146,318,938 of AC_000072).
Gene_expression (overexpressing) of Ku70 in nervous system associated with targeted disruption
5) Confidence 0.51 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.26 Pain Relevance 0
For genotyping of Ku70 transgenic mice, PCR was performed with the primers (forward) 5?
Gene_expression (transgenic mice) of Ku70 associated with targeted disruption
6) Confidence 0.51 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.55 Pain Relevance 0
Ku70-shRNA knockdown and EGFP-Htt expression vectors
Gene_expression (expression) of Ku70
7) Confidence 0.51 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.13 Pain Relevance 0
This suggests that, after a short-term transient fluctuation at the embryonic stage, the Ku70 protein expression level becomes stable at the equilibrium between increased synthesis and increased degradation of Ku70.
Gene_expression (synthesis) of Ku70 in embryonic stage
8) Confidence 0.51 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.21 Pain Relevance 0
We next asked whether functional recovery of Ku70 by increasing the amount of Ku70 rescues neurodegeneration in vivo.
Gene_expression (recovery) of Ku70
9) Confidence 0.51 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.14 Pain Relevance 0
After three steps of washing, Ku70 antibody was added followed by the HRP-conjugated secondary antibody.
Gene_expression (antibody) of Ku70
10) Confidence 0.51 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0 Pain Relevance 0.08
It is of note that Ku70 signal levels in the nucleoplasm of inclusion-positive neurons were not reduced in vitro and in vivo (Fig. 4, A and B).
Gene_expression (levels) of Ku70 in neurons
11) Confidence 0.46 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.22 Pain Relevance 0
The expression of Ku70-Flag protein was detected in the striatum of transgenic mice by Western blot analysis (Fig. 8 B) and immunohistochemistry (Fig. 8 C).
Gene_expression (expression) of Ku70 in striatum associated with targeted disruption
12) Confidence 0.46 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.45 Pain Relevance 0.08
In addition to the direct inhibition of DNA–PK activity through binding to Ku70, mutant Htt might influence expression levels of Ku70 or of other components in the DNA–PK complex.
Gene_expression (expression) of Ku70
13) Confidence 0.46 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.07 Pain Relevance 0
Western blot analysis with primary cortical neurons expressing mutant Htt showed that protein levels of Ku70 and Ku80 but not of DNA–PKcs transiently increased at day 1, decreased from day 1 to day 6 after infection, and returned to the initial level (Fig. 6, A–C).
Gene_expression (levels) of Ku70 in neurons associated with infection
14) Confidence 0.46 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.10 Pain Relevance 0
In addition to the direct inhibition of DNA–PK activity through binding to Ku70, mutant Htt might influence expression levels of Ku70 or of other components in the DNA–PK complex.
Gene_expression (levels) of Ku70
15) Confidence 0.46 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.07 Pain Relevance 0
However, acetylated Ku70 was not found in cortical neurons before or after expression of mutant Htt (unpublished data).
Neg (not) Gene_expression (found) of Ku70 in neurons
16) Confidence 0.46 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.24 Pain Relevance 0
S1 C, HEK293 cells were cultured in DME supplemented with 10% fetal bovine serum at 37°C and 5% CO2. pFlag-CMV-D11-Htt (N-terminal 506 amino acids containing 145 glutamines) and pcMyc-CMV-D12-Ku70 were cotransfected using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions.
Gene_expression (cotransfected) of Ku70 associated with cytomegalovirus infection
17) Confidence 0.46 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.26 Pain Relevance 0
We generated transgenic mice overexpressing Ku70-Flag protein under the control of the neuron-specific enolase (NSE) enhancer/promoter (Fig. 8 A).
Gene_expression (overexpressing) of Ku70 in neuron associated with targeted disruption
18) Confidence 0.46 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.36 Pain Relevance 0.07
Instead, we generated double-heterotransgenic mice (Ku70+/?
Gene_expression (/) of Ku70
19) Confidence 0.45 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.13 Pain Relevance 0
Instead, we generated double-heterotransgenic mice (Ku70+/?
Gene_expression (heterotransgenic) of Ku70
20) Confidence 0.45 Published 2010 Journal The Journal of Cell Biology Section Body Doc Link PMC2867301 Disease Relevance 0.13 Pain Relevance 0

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