INT312888

Context Info Confidence 0.73 First Reported 2010 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 9 Disease Relevance 0 Pain Relevance 0.03

cytosol (Hdac6) nucleolus (Hdac6) nucleus (Hdac6) intracellular (Hdac6) protein complex (Hdac6) cytoplasm (Hdac6)

Hdac6 (Rattus norvegicus)

Pain Link Frequency Relevance Heat agonist 18 55.28 Serotonin 18 27.68 fluoxetine 27 9.52 sSRI 18 8.40 imagery 45 5.00 cerebral cortex 9 5.00 depression 9 5.00 Hippocampus 9 5.00

Disease Link Frequency Relevance Heat Disease 126 5.00 Neurodegenerative Disease 18 5.00 Infection 18 5.00 Depression 9 5.00 Stress 9 5.00 Targeted Disruption 9 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Further, a genome-wide phosphoprotein survey has shown that this site on HDAC6 is phosphorylated [18].

Phosphorylation (phosphorylated) of HDAC6

1) Confidence 0.73 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877100 Disease Relevance 0 Pain Relevance 0

may directly phosphorylate HDAC6 at this site, although further work with purified proteins is needed to determine whether this is the case.

Phosphorylation (phosphorylate) of HDAC6

2) Confidence 0.73 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877100 Disease Relevance 0 Pain Relevance 0

The function of HDAC6 phosphorylation

Phosphorylation (phosphorylation) of HDAC6

3) Confidence 0.73 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877100 Disease Relevance 0 Pain Relevance 0

Using a phosphoepitope-specific antibody, we showed that phosphorylation of HDAC6 at serine-22, which conforms to the consensus site for GSK3?

Phosphorylation (phosphorylation) of HDAC6

4) Confidence 0.73 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877100 Disease Relevance 0 Pain Relevance 0

Using a commercially available antibody (Abcam, ab61058, HDAC6-phospho S22), we found that inhibiting GSK3?

Phosphorylation (phospho) of HDAC6

5) Confidence 0.72 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877100 Disease Relevance 0 Pain Relevance 0.03

Various phosphorylation site prediction methods have indicated that serine-22 in murine and rat HDAC6 (corresponding to serine-21 in human HDAC6) may be phosphorylated by GSK3?.

Phosphorylation (phosphorylated) of HDAC6

6) Confidence 0.72 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877100 Disease Relevance 0 Pain Relevance 0

Using a phosphoepitope-specific antibody, we showed that phosphorylation of HDAC6 at serine-22, which conforms to the consensus site for GSK3?

Phosphorylation (phosphorylation) of HDAC6

7) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877100 Disease Relevance 0 Pain Relevance 0

It has been shown that HDAC6 is a phosphoprotein [18], [19], but neither the kinases that are responsible for phosphorylating HDAC6 nor the effect of phosphorylation on this deacetylase have been established.

Phosphorylation (phosphorylation) of HDAC6

8) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877100 Disease Relevance 0 Pain Relevance 0

It has been shown that HDAC6 is a phosphoprotein [18], [19], but neither the kinases that are responsible for phosphorylating HDAC6 nor the effect of phosphorylation on this deacetylase have been established.

Phosphorylation (phosphorylating) of HDAC6

9) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2877100 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.