INT313308

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Context Info
Confidence 0.46
First Reported 2010
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 9
Disease Relevance 1.16
Pain Relevance 0.10

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytoplasm (SQSTM1, KEAP1) nucleus (SQSTM1, KEAP1) cell differentiation (SQSTM1)
nucleoplasm (SQSTM1) endoplasmic reticulum (KEAP1) response to stress (SQSTM1)
Anatomy Link Frequency
HeLa 1
SQSTM1 (Homo sapiens)
KEAP1 (Homo sapiens)
Pain Link Frequency Relevance Heat
Osteoarthritis 9 69.36 Quite High
Pain 9 68.72 Quite High
Glutamate 9 36.40 Quite Low
Disease Link Frequency Relevance Heat
Stress 63 97.96 Very High Very High Very High
Bone Disease 27 96.88 Very High Very High Very High
Repression 9 89.88 High High
Spontaneous Fractures 9 70.44 Quite High
Osteoarthritis 9 69.36 Quite High
Pain 9 68.72 Quite High
Adenocarcinoma 18 65.84 Quite High
Hepatocellular Cancer 18 62.48 Quite High
Neuroblastoma 9 39.44 Quite Low
Toxicity 9 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To confirm the association of Keap1 with SQSTM1, we expressed human Keap1-V5/His or SQSTM1-FLAG/His in HEK293T or HeLa cells and subjected cell lysates to anti-V5 or anti-FLAG pulldown followed by immunoblotting for Keap1 or SQSTM1.
SQSTM1 Binding (association) of Keap1 in HeLa
1) Confidence 0.46 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
Here, we have used immunopurification of Keap1 and mass spectrometry, in addition to immunoblotting, to identify sequestosome 1 (SQSTM1) as a cellular binding partner of Keap1.
SQSTM1 Binding (partner) of Keap1
2) Confidence 0.46 Published 2010 Journal The Journal of Biological Chemistry Section Abstract Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
Endogenous Keap1 was identified as a co-precipitating protein (Fig. 1C), demonstrating that SQSTM1 is a binding partner of Keap1 under physiological conditions.


SQSTM1 Binding (partner) of Keap1
3) Confidence 0.36 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.24 Pain Relevance 0
Here, we have used immunopurification of Keap1 and mass spectrometry, in addition to immunoblotting, to identify sequestosome 1 (SQSTM1) as a cellular binding partner of Keap1.
sequestosome 1 Binding (partner) of Keap1
4) Confidence 0.36 Published 2010 Journal The Journal of Biological Chemistry Section Abstract Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
In this study, we have shown that SQSTM1, whose major functions include (i) serving as a scaffold in various signaling pathways through its multiple protein-protein interaction motifs, (ii) sequestering damaged and misfolded proteins into aggregates prior to their degradation, and (iii) shuttling polyubiquitinated proteins to the proteasomal and lysosomal degradation machineries (18), interacts both physically and functionally with Keap1, the negative regulator of the cytoprotective transcription factor Nrf2.
SQSTM1 Binding (interacts) of Keap1
5) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.10 Pain Relevance 0
In a very recent report (23), Komatsu and colleagues have demonstrated an interaction between Keap1 and SQSTM1 and have defined the motifs in Keap1 and SQSTM1 responsible for this interaction.
SQSTM1 Binding (interaction) of Keap1
6) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.15 Pain Relevance 0
These findings demonstrate a physical and functional interaction between Keap1 and SQSTM1 and reveal an additional layer of regulation in the Keap1-Nrf2 pathway.



SQSTM1 Binding (interaction) of Keap1
7) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Abstract Doc Link PMC2878012 Disease Relevance 0 Pain Relevance 0
In summary, this study has presented evidence for a physical and functional interaction between Keap1 and SQSTM1 in mammalian cells.
SQSTM1 Binding (interaction) of Keap1
8) Confidence 0.34 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.43 Pain Relevance 0.10
Endogenous Keap1 was identified as a co-precipitating protein (Fig. 1C), demonstrating that SQSTM1 is a binding partner of Keap1 under physiological conditions.


SQSTM1 Binding (partner) of Keap1
9) Confidence 0.31 Published 2010 Journal The Journal of Biological Chemistry Section Body Doc Link PMC2878012 Disease Relevance 0.24 Pain Relevance 0

General Comments

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