INT313711

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Context Info
Confidence 0.76
First Reported 2010
Last Reported 2011
Negated 0
Speculated 1
Reported most in Body
Documents 4
Total Number 14
Disease Relevance 1.91
Pain Relevance 2.29

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
Pet1 6
neurons 4
adult brain 1
serotonergic neurons 1
Pet1 (Mus musculus)
Pain Link Frequency Relevance Heat
medulla 33 98.48 Very High Very High Very High
Raphe 181 98.20 Very High Very High Very High
Serotonin 81 97.04 Very High Very High Very High
Dopamine 88 95.20 Very High Very High Very High
Neurotransmitter 22 93.00 High High
monoamine 24 87.08 High High
imagery 32 80.48 Quite High
fluoxetine 37 71.36 Quite High
depression 12 67.44 Quite High
anesthesia 12 62.96 Quite High
Disease Link Frequency Relevance Heat
Glioblastoma 10 95.36 Very High Very High Very High
Colon Cancer 3 92.08 High High
Targeted Disruption 31 88.28 High High
Cancer 81 79.36 Quite High
Mental Disorders 33 69.52 Quite High
Depression 12 67.44 Quite High
Post-traumatic Stress Disorder 11 66.24 Quite High
Recurrence 3 64.96 Quite High
Body Weight 33 64.92 Quite High
Metastasis 2 63.44 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To test whether deleting Lmx1b decreases the number of 5-HTergic neurons or not, which in turn results in the phenotypes mentioned above, we examined the expression of Aadc and Pet1.
Spec (examined) Gene_expression (expression) of Pet1 in Pet1
1) Confidence 0.76 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0 Pain Relevance 0.11
It is likely that Lmx1b and Pet1 act in parallel to regulate central 5-HTergic system, the expression of Pet1 in adult brain is independent of Lmx1b, and Pet1 is not involved in alterations of gene expression in Lmx1b iCKO mice.
Gene_expression (expression) of Pet1 in Pet1
2) Confidence 0.76 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0.20 Pain Relevance 0.15
In the present study, normal Pet1 expression was found in Lmx1b iCKO mice.
Gene_expression (expression) of Pet1 in Pet1
3) Confidence 0.76 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0.17 Pain Relevance 0.15
Generation of Pet1-CreERT2 mice
Gene_expression (Generation) of Pet1 in Pet1
4) Confidence 0.76 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0.07 Pain Relevance 0
Furthermore, Pet1 expression in 5-HTergic neurons requires Lmx1b during embryonic development [12], but its expression in the raphe nuclei of Lmx1b iCKO mice showed no difference from wild-type controls (Figure 5Q–T).
Gene_expression (expression) of Pet1 in neurons associated with raphe
5) Confidence 0.76 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0 Pain Relevance 0.22
Both Lmx1b and Pet1 are expressed in postmitotic 5-HTergic neurons and essential for the differentiation and survival of 5-HTergic neurons during embryonic development [4], [11], [12].
Gene_expression (expressed) of Pet1 in neurons
6) Confidence 0.76 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0.12 Pain Relevance 0.19
In contrast, the expression of both Pet1 and Aadc appeared unchanged in Lmx1b iCKO mice relative to control mice, indicating that there was no loss of 5-HTergic neurons in the raphe nuclei.
Gene_expression (expression) of Pet1 in neurons associated with raphe
7) Confidence 0.76 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0 Pain Relevance 0.13
and Pet1-Cre; Lmx1bflox/?
Gene_expression (/) of Pet1-Cre in Pet1
8) Confidence 0.66 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0 Pain Relevance 0.12
To specifically inactivate Lmx1b expression in 5-HTergic neurons in the adult mouse brain, Lmx1bflox/flox mice were crossed with Pet1-CreERT2 mice (see below) and their offspring Pet1-CreERT2; Lmx1bflox/+ mice were then crossed with one another to obtain Pet1-CreERT2; Lmx1bflox/flox (Lmx1b iCKO) mice.
Gene_expression (crossed) of Pet1 in Pet1
9) Confidence 0.66 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0 Pain Relevance 0
Pet1 is expressed specifically in central 5-HTergic neurons [4], and the distribution of Cre-recombination activity was determined by crossing to Rosa26R mice [15].
Gene_expression (expressed) of Pet1 in neurons
10) Confidence 0.59 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0.21 Pain Relevance 0.12
These results suggest that the overall number of 5-HTergic neurons is not affected by deleting Lmx1b in adulthood, and that Pet1 expression in the adult brain is independent of Lmx1b.


Gene_expression (expression) of Pet1 in adult brain
11) Confidence 0.59 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3016403 Disease Relevance 0 Pain Relevance 0.37
The transcription factor Pet1 is expressed in serotonergic neurons and directly activates the transcription of genes that are involved in the synthesis (Tph and Aadc) and uptake (Sert) of serotonin.
Gene_expression (expressed) of Pet1 in serotonergic neurons associated with serotonin
12) Confidence 0.41 Published 2010 Journal EMBO J Section Body Doc Link PMC2944059 Disease Relevance 0 Pain Relevance 0.53
For the Pet-1 experiment, sequences that passed all three of our quality filters were sorted and counted using Textpad.
Gene_expression (experiment) of Pet-1
13) Confidence 0.28 Published 2010 Journal Nucleic Acids Research Section Body Doc Link PMC2879535 Disease Relevance 0 Pain Relevance 0.07
Not surprisingly, visualization and quantification of CD133 in overexpressing U251 xenografts was successful; more importantly, however, significant differences were also found in matched HCT116 xenograft pairs, despite the lower CD133 expression levels.
Gene_expression (overexpressing) of U251
14) Confidence 0.01 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC3004948 Disease Relevance 1.14 Pain Relevance 0.11

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