INT318271

Context Info Confidence 0.44 First Reported 2010 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 7 Total Number 8 Disease Relevance 2.74 Pain Relevance 3.28

aging (SLC32A1) plasma membrane (SLC32A1) transmembrane transport (SLC32A1)

Anatomy Link Frequency epithelium 2 SLC32A1 (Homo sapiens)

Pain Link Frequency Relevance Heat gABA 334 100.00 Cannabinoid receptor 84 100.00 GABAergic 506 98.68 Central nervous system 6 97.60 agonist 4 71.36 Nicotine 18 5.00 fluoxetine 12 5.00 Pyramidal cell 12 5.00 Glutamate 10 5.00 Neurotransmitter 8 5.00

Disease Link Frequency Relevance Heat Lifespan 162 99.44 Nicotine Addiction 136 93.16 Aging 102 78.04 Stress 4 62.72 Anxiety Disorder 6 57.00 Generalized Anxiety Disorder 16 37.20 Amblyopia 42 5.00 Asthma 8 5.00 Cataract 6 5.00 Cognitive Disorder 6 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

We quantified the inhibitory modulating cannabinoid receptor (CB1), GABA vesicular transporter (VGAT), GABA synthesizing enzymes (GAD65/GAD67), GABAA receptor anchoring protein (Gephyrin), and GABAA receptor subunits (GABAA?

Gene_expression (synthesizing) of VGAT associated with gaba and cannabinoid receptor

1) Confidence 0.44 Published 2010 Journal Frontiers in Cellular Neuroscience Section Abstract Doc Link PMC2893712 Disease Relevance 0.29 Pain Relevance 0.39

To quantify changes in the balance between the mechanisms that produce and traffic GABA in the pre-synaptic terminal, we calculated an index of GAD65:VGAT expression.

Gene_expression (expression) of VGAT associated with gaba

2) Confidence 0.44 Published 2010 Journal Frontiers in Cellular Neuroscience Section Body Doc Link PMC2893712 Disease Relevance 0.43 Pain Relevance 0.36

To further examine how the pre-synaptic components of the GABAergic system change across the lifespan in human V1, we quantified the expression of two key inhibitory proteins, CB1 and VGAT (Figure 2).

Gene_expression (expression) of VGAT associated with cannabinoid receptor, lifespan and gabaergic

3) Confidence 0.44 Published 2010 Journal Frontiers in Cellular Neuroscience Section Body Doc Link PMC2893712 Disease Relevance 0.48 Pain Relevance 0.51

VGAT expression was highest in infants (<1 year) and pre-teens (5–11 years), and had about 32% less expression in adults (21–55 years; p <?

Gene_expression (expression) of VGAT

4) Confidence 0.44 Published 2010 Journal Frontiers in Cellular Neuroscience Section Body Doc Link PMC2893712 Disease Relevance 0.45 Pain Relevance 0.37

In the second stage, the teenage transition was a switch from a greater capacity for trafficking GABA (more VGAT) to a greater capacity to produce the on-demand pool of GABA (more GAD65).

Gene_expression (produce) of VGAT associated with gaba

5) Confidence 0.42 Published 2010 Journal Frontiers in Cellular Neuroscience Section Body Doc Link PMC2893712 Disease Relevance 0.06 Pain Relevance 0.17

Interestingly, in the human airway epithelium there is no VGAT expression, suggesting GABA is released from airway epithelial cells in a vesicle independent fashion [18].

Gene_expression (expression) of VGAT in epithelium associated with gaba

6) Confidence 0.41 Published 2010 Journal Respir Res Section Body Doc Link PMC2988726 Disease Relevance 0.13 Pain Relevance 0.61

In regard to transporters, there was no GAT-3 and VGAT expression in human large and small airway epithelium.

Gene_expression (expression) of VGAT in epithelium

7) Confidence 0.41 Published 2010 Journal Respir Res Section Body Doc Link PMC2988726 Disease Relevance 0.42 Pain Relevance 0.56

There was more VGAT expression in both young children (<11 years) and older adults (>55 years; Figure 3).

Gene_expression (expression) of VGAT

8) Confidence 0.38 Published 2010 Journal Frontiers in Cellular Neuroscience Section Body Doc Link PMC2893712 Disease Relevance 0.48 Pain Relevance 0.31

General Comments

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