INT325899

Context Info Confidence 0.12 First Reported 2010 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 6 Disease Relevance 2.43 Pain Relevance 0

pigmentation (Hps3) peptidase activity (F2) extracellular space (F2) extracellular region (F2) cytoplasm (Hps3)

Anatomy Link Frequency blood 1 Hps3 (Mus musculus) F2 (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 12 5.00 Pain 6 5.00 Arthritis 6 5.00

Disease Link Frequency Relevance Heat Coagulation Disorder 108 97.76 Abscess 378 96.32 Sprains And Strains 150 95.72 Bacteremia 60 92.92 Sepsis 36 92.72 Disease 78 89.04 Immunization 54 51.04 Yersinia Infection 6 26.88 Staphylococcus Infection 90 5.00 Infection 90 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

For the coagulase-prothrombin interaction, coagulase was injected at 10 pM, 0.1 nM, 1 nM, 5 nM, 10 nM, 25 nM, and 50 nM for 300 seconds followed by 300 seconds of dissociation followed by regeneration with NaOH (50 µL, 30 seconds).

coa Binding (interaction) of prothrombin

1) Confidence 0.12 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.05 Pain Relevance 0

Clinical isolates of the human pathogen Staphylococcus aureus secrete coagulase (Coa), a polypeptide that binds to and activates prothrombin, thereby converting fibrinogen to fibrin and promoting clotting of plasma or blood.

coa Binding (binds) of prothrombin in blood associated with coagulation disorder

2) Confidence 0.12 Published 2010 Journal PLoS Pathogens Section Abstract Doc Link PMC2916881 Disease Relevance 0.93 Pain Relevance 0

The KD for the coagulase·prothrombin interaction is lower than the reported affinity for the D1D2 domain alone [36]; we currently do not appreciate the basis for this difference.

coa Binding (interaction) of prothrombin

3) Confidence 0.12 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.25 Pain Relevance 0

For the coagulase-prothrombin interaction, coagulase was injected at 10 pM, 0.1 nM, 1 nM, 5 nM, 10 nM, 25 nM, and 50 nM for 300 seconds followed by 300 seconds of dissociation followed by regeneration with NaOH (50 µL, 30 seconds).

coa Binding (interaction) of prothrombin

4) Confidence 0.12 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.05 Pain Relevance 0

The KD for the coagulase·prothrombin interaction is lower than the reported affinity for the D1D2 domain alone [36]; we currently do not appreciate the basis for this difference.

coa Binding (interaction) of prothrombin

5) Confidence 0.12 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.25 Pain Relevance 0

Antibodies directed against Coa or vWbp prevent coagulase binding to prothrombin or fibrinogen and confer protection against challenge with S. aureus Newman or the methicillin-resistant S. aureus isolate USA300 LAC in mouse models of abscess formation or lethal sepsis.

coa Binding (binding) of prothrombin associated with abscess and sepsis

6) Confidence 0.12 Published 2010 Journal PLoS Pathogens Section Abstract Doc Link PMC2916881 Disease Relevance 0.90 Pain Relevance 0

General Comments

This test has worked.