INT325899
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
For the coagulase-prothrombin interaction, coagulase was injected at 10 pM, 0.1 nM, 1 nM, 5 nM, 10 nM, 25 nM, and 50 nM for 300 seconds followed by 300 seconds of dissociation followed by regeneration with NaOH (50 µL, 30 seconds). | |||||||||||||||
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Clinical isolates of the human pathogen Staphylococcus aureus secrete coagulase (Coa), a polypeptide that binds to and activates prothrombin, thereby converting fibrinogen to fibrin and promoting clotting of plasma or blood. | |||||||||||||||
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The KD for the coagulase·prothrombin interaction is lower than the reported affinity for the D1D2 domain alone [36]; we currently do not appreciate the basis for this difference. | |||||||||||||||
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For the coagulase-prothrombin interaction, coagulase was injected at 10 pM, 0.1 nM, 1 nM, 5 nM, 10 nM, 25 nM, and 50 nM for 300 seconds followed by 300 seconds of dissociation followed by regeneration with NaOH (50 µL, 30 seconds). | |||||||||||||||
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The KD for the coagulase·prothrombin interaction is lower than the reported affinity for the D1D2 domain alone [36]; we currently do not appreciate the basis for this difference. | |||||||||||||||
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Antibodies directed against Coa or vWbp prevent coagulase binding to prothrombin or fibrinogen and confer protection against challenge with S. aureus Newman or the methicillin-resistant S. aureus isolate USA300 LAC in mouse models of abscess formation or lethal sepsis. | |||||||||||||||
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General Comments
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