INT325906

Context Info Confidence 0.02 First Reported 2010 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 4 Disease Relevance 0.96 Pain Relevance 0

pigmentation (Hps3) signal transduction (Fgg) extracellular space (Fgg) extracellular region (Fgg) protein binding, bridging (Fgg) cytoplasm (Hps3)

Hps3 (Mus musculus) Fgg (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 8 5.00 Pain 4 5.00 Arthritis 4 5.00

Disease Link Frequency Relevance Heat Abscess 252 96.32 Sprains And Strains 100 95.72 Sepsis 24 93.28 Disease 52 89.04 Coagulation Disorder 72 80.28 Bacteremia 40 67.84 Immunization 36 59.00 Staphylococcus Infection 60 5.00 Infection 60 5.00 Mrsa Infection 24 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

To measure the interaction of coagulase with prothrombin and fibrinogen, Coa was diluted into HBS-P buffer (20 mM HEPES [pH 7.4], 150 mM NaCl, 0.005% [vol/vol] surfactant P20) at a range of concentrations.

coa Binding (interaction) of fibrinogen

1) Confidence 0.02 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0.06 Pain Relevance 0

For the coagulase-fibrinogen interaction, coagulase was injected at 100 pM, 500 pM, 1 nM, 5 nM, 10 nM, 25 nM, 50 nM, 100 nM, and 300 nM for 60 seconds of association followed by 60 seconds of dissociation and the chip was regenerated as described above.

coa Binding (interaction) of fibrinogen

2) Confidence 0.02 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0 Pain Relevance 0

For the coagulase-fibrinogen interaction, coagulase was injected at 100 pM, 500 pM, 1 nM, 5 nM, 10 nM, 25 nM, 50 nM, 100 nM, and 300 nM for 60 seconds of association followed by 60 seconds of dissociation and the chip was regenerated as described above.

coa Binding (interaction) of fibrinogen

3) Confidence 0.02 Published 2010 Journal PLoS Pathogens Section Body Doc Link PMC2916881 Disease Relevance 0 Pain Relevance 0

Antibodies directed against Coa or vWbp prevent coagulase binding to prothrombin or fibrinogen and confer protection against challenge with S. aureus Newman or the methicillin-resistant S. aureus isolate USA300 LAC in mouse models of abscess formation or lethal sepsis.

coa Binding (binding) of fibrinogen associated with abscess and sepsis

4) Confidence 0.02 Published 2010 Journal PLoS Pathogens Section Abstract Doc Link PMC2916881 Disease Relevance 0.91 Pain Relevance 0

General Comments

This test has worked.