INT325906
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
To measure the interaction of coagulase with prothrombin and fibrinogen, Coa was diluted into HBS-P buffer (20 mM HEPES [pH 7.4], 150 mM NaCl, 0.005% [vol/vol] surfactant P20) at a range of concentrations. | |||||||||||||||
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For the coagulase-fibrinogen interaction, coagulase was injected at 100 pM, 500 pM, 1 nM, 5 nM, 10 nM, 25 nM, 50 nM, 100 nM, and 300 nM for 60 seconds of association followed by 60 seconds of dissociation and the chip was regenerated as described above. | |||||||||||||||
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For the coagulase-fibrinogen interaction, coagulase was injected at 100 pM, 500 pM, 1 nM, 5 nM, 10 nM, 25 nM, 50 nM, 100 nM, and 300 nM for 60 seconds of association followed by 60 seconds of dissociation and the chip was regenerated as described above. | |||||||||||||||
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Antibodies directed against Coa or vWbp prevent coagulase binding to prothrombin or fibrinogen and confer protection against challenge with S. aureus Newman or the methicillin-resistant S. aureus isolate USA300 LAC in mouse models of abscess formation or lethal sepsis. | |||||||||||||||
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General Comments
This test has worked.