INT335675

From wiki-pain
Jump to: navigation, search
Context Info
Confidence 0.63
First Reported 2010
Last Reported 2010
Negated 1
Speculated 1
Reported most in Body
Documents 1
Total Number 27
Disease Relevance 1.23
Pain Relevance 0.20

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

unfolded protein binding (Calr) Golgi apparatus (Calr) endoplasmic reticulum (Calr)
cytoplasm (Calr) cytosol (Calr) protein folding (Calr)
Anatomy Link Frequency
CPA 5
plasma 1
cleavage 1
Calr (Mus musculus)
Pain Link Frequency Relevance Heat
Calcium channel 54 77.12 Quite High
ketamine 27 67.28 Quite High
anesthesia 27 65.52 Quite High
Glutamate 27 18.64 Low Low
Disease Link Frequency Relevance Heat
American Trypanosomiasis 108 99.58 Very High Very High Very High
Cancer 27 97.08 Very High Very High Very High
Stress 27 92.72 High High
Wound Healing 27 88.88 High High
Adhesions 27 81.44 Quite High
Togavirus Infection 27 70.08 Quite High
Coronary Heart Disease 27 64.96 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Here we show that in Trypanosoma cruzi a minor fraction of CRT localized to the cytosol.
Localization (localized) of CRT associated with american trypanosomiasis
1) Confidence 0.63 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2950133 Disease Relevance 0.17 Pain Relevance 0
Finally, evidence presented here and previous results by others suggest that the regulation of CRT retrotranslocation by calcium could be a crossroad between diverse signaling pathways.
Localization (retrotranslocation) of CRT
2) Confidence 0.63 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
However, at variance with ERAD substrates and bacterial toxins, we propose that CRT retrotranslocation is modulated by structural modifications triggered by variations of ER calcium levels.
Localization (retrotranslocation) of CRT
3) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0.03
Moreover, the fact that enhancement of cytosolic CRT content triggered by CPA treatment occurred even in the absence of protein synthesis also favors the idea that CRT is retrotranslocated from the ER lumen to the cytosol.
Localization (retrotranslocated) of CRT in CPA
4) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
The arrival of a signal that mobilizes ER calcium would trigger an increment of cytosolic calcium and favor CRT retrotranslocation, thus fulfilling both known conditions for CRT-mediated nuclear receptor export.
Localization (export) of CRT-mediated
5) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
To analyze which of both phenomena (decrease of ER calcium or its cytosolic increase) triggered TcCRT relocalization, parasites were incubated with 5 mM CaCl2 and 10 ┬ÁM A23187.
Localization (relocalization) of TcCRT
6) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
It may be speculated that ER calcium mobilization might promote TcCRT secretion and that this phenomenon may explain, by cross contamination, the increase observed in cytosolic TcCRT.
Localization (secretion) of TcCRT
7) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
Furthermore, they show that TcCRT was translocated to the cytosol and not sorted to lysosomes upon CPA treatment.
Neg (not) Localization (translocated) of TcCRT in CPA
8) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
According to this model, CRT start to translocate into the ER during their synthesis, but after cleavage of the signal peptide some ribosomes detach from the ER membrane and the synthesis is completed in the cytosol in free ribosomes [30].
Localization (translocate) of CRT in cleavage
9) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0.38 Pain Relevance 0
We also found that a shorter version of TcCRT lacking the C-terminal domain was totally retained in the ER, whereas a non related protein such as GFP fused to this same domain mimicked TcCRT localization.
Localization (localization) of TcCRT
10) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0.03
As a first approach to study the intracellular localization of TcCRT we separated the ER from the cytosol plus lysosomal contents by freezing the parasites at ?
Localization (localization) of TcCRT
11) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
Although this suggestion is ruled out by the fact that parasites were washed twice with PBS before subcellular fractionation, it might be further speculated that an increased TcCRT secretion might in turn result in an increased TcCRT unspecific adherence to T. cruzi plasma membrane, which in turn would contaminate in some way the cytosolic fraction.
Localization (secretion) of TcCRT in plasma
12) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
For instance, secreted CRT is involved in the modulation of the immunogenic response towards dying cancer cells [14].
Localization (secreted) of CRT associated with cancer
13) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0.39 Pain Relevance 0
To analyze the kinetics TcCRT translocation as a result SERCA inhibition, cells were incubated in the presence of CPA and harvested at different time points.
Spec (analyze) Localization (translocation) of TcCRT in CPA
14) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
The arrival of a signal that mobilizes ER calcium would trigger an increment of cytosolic calcium and favor CRT retrotranslocation, thus fulfilling both known conditions for CRT-mediated nuclear receptor export.
Localization (retrotranslocation) of CRT
15) Confidence 0.55 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
These results show that ER calcium level regulates the C-terminal-dependent retrotranslocation of TcCRT.
Localization (retrotranslocation) of TcCRT
16) Confidence 0.51 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0.04
The presence of TcCRT in the cytosol may be a consequence of retrotranslocation or the result of an inefficient translocation during protein synthesis.
Localization (retrotranslocation) of TcCRT
17) Confidence 0.51 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
For this reason, the biological relevance of cytosolic CRT is controversial.
Localization (relevance) of CRT
18) Confidence 0.51 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
Calcium induced TcCRT retrotranslocation depends on its C-terminal domain
Localization (retrotranslocation) of TcCRT
19) Confidence 0.51 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
To evaluate whether ER calcium levels could influence TcCRT localization, we studied its variation resulting from ER calcium mobilization produced by the inhibition of the ER SERCA pump by CPA.
Localization (localization) of TcCRT in CPA
20) Confidence 0.51 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.

Personal tools
Namespaces

Variants
Actions
Navigation
Toolbox