INT340600

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Context Info
Confidence 0.68
First Reported 2010
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 1
Total Number 17
Disease Relevance 2.14
Pain Relevance 9.14

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

extracellular matrix organization (Wnt3a) extracellular space (Wnt3a) extracellular region (Wnt3a)
proteinaceous extracellular matrix (Wnt3a) plasma membrane (Wnt3a) molecular_function (Wnt3a)
Anatomy Link Frequency
dentate gyrus 3
Brains 1
Wnt3a (Rattus norvegicus)
Pain Link Frequency Relevance Heat
fluoxetine 1122 99.24 Very High Very High Very High
antagonist 34 98.12 Very High Very High Very High
Intracerebroventricular 17 96.96 Very High Very High Very High
antidepressant 85 87.48 High High
Serotonin 51 86.40 High High
sSRI 85 79.72 Quite High
depression 85 77.68 Quite High
Hippocampus 51 74.48 Quite High
Disease Link Frequency Relevance Heat
Neurodegenerative Disease 323 99.08 Very High Very High Very High
Targeted Disruption 68 81.72 Quite High
Depression 85 77.68 Quite High
Volume Depletion And Dehydration 17 77.00 Quite High
Sprains And Strains 17 35.40 Quite Low
Anxiety Disorder 17 30.64 Quite Low
Hypersensitivity 85 5.00 Very Low Very Low Very Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The second experiment began by confirming, under our conditions, that a 7-day treatment with fluoxetine had no discernible effect on progenitor mitosis, or pCREB and Wnt3a expression, whereas after 14 days all three were stimulated.
Gene_expression (expression) of Wnt3a associated with fluoxetine
1) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0.28 Pain Relevance 0.56
The role of BDNF in the expression of pCREB and Wnt3a may be distinct.
Gene_expression (expression) of Wnt3a
2) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0.31 Pain Relevance 0.36
However, it did not prevent increased Wnt3a expression, suggesting that the latter is not a sufficient event for increased progenitor cell mitosis.
Gene_expression (expression) of Wnt3a
3) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0.38 Pain Relevance 0.48
These experiments show that (i) Fluoxetine increased Ki67 counts, as well as pCREB and Wnt3a expression in the dentate gyrus.
Gene_expression (expression) of Wnt3a in dentate gyrus associated with fluoxetine
4) Confidence 0.68 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2965105 Disease Relevance 0.07 Pain Relevance 0.62
In contrast, the expression of Wnt3a, also known to be closely involved in neurogenesis [3], [22], [33], [34] was, as expected, increased by fluoxetine, but this was not prevented by K252a.
Gene_expression (expression) of Wnt3a associated with neurodegenerative disease and fluoxetine
5) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0.33 Pain Relevance 0.44
This paper explores the role of BDNF in this process, using the effect of a Trk antagonist (K252a) on the labelling of progenitor cells with the mitosis marker Ki67 and the associated expression of pCREB and Wnt3a.
Gene_expression (expression) of Wnt3a associated with antagonist
6) Confidence 0.68 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2965105 Disease Relevance 0.07 Pain Relevance 0.62
However, adding K252a to fluoxetine did not prevent the latter's stimulating effect on Wnt3a expression (Figure 1D).
Gene_expression (expression) of Wnt3a associated with fluoxetine
7) Confidence 0.68 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0 Pain Relevance 0.86
Increased pCREB was always associated with progenitor cell mitosis, but Wnt3a expression may be necessary but not sufficient for increased progenitor cell proliferation.
Gene_expression (expression) of Wnt3a
8) Confidence 0.68 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2965105 Disease Relevance 0.05 Pain Relevance 0.45
As in Experiment 1, pCREB expression was inhibited by 14 days K252a treatment (not shown in Figure 3, since there was effectively no signal in the K252a treated animals) but Wnt3a expression was not altered, even though Ki67 expression was reduced (Figure 3B).


Gene_expression (expression) of Wnt3a
9) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0 Pain Relevance 0.56
There was also no observable change in either pCREB or Wnt3a at 7 days, but by 14 days the expression of both was clearly increased (Figure 2C, D).


Gene_expression (expression) of Wnt3a
10) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0 Pain Relevance 0.90
The first part of this experiment showed that 7 days treatment with fluoxetine had no effect on Ki67 labelled cells, or on the expression of either pCREB or Wnt3a.
Gene_expression (expression) of Wnt3a associated with fluoxetine
11) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0 Pain Relevance 0.54
For single staining with Wnt3a, sections were incubated in rabbit anti Wnt3a (Abcam, Science Park, Cambridge UK; 5 ug/ml in 0.5% Triton with 1% goat serum) overnight in a humidified chamber at room temperature.
Gene_expression (staining) of Wnt3a
12) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0.08 Pain Relevance 0
Brains were examined for Ki67, pCREB, BDNF and Wnt3a.
Gene_expression (examined) of Wnt3a in Brains
13) Confidence 0.59 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0 Pain Relevance 0.40
Table 1 summarises the results of these two experiments on the expression of Ki67, pCREB and Wnt3a in the dentate gyrus.



Gene_expression (expression) of Wnt3a in dentate gyrus
14) Confidence 0.53 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0.08 Pain Relevance 0.57
As in experiment 1, pCREB was reduced in fluoxetine and K252a-treated animals, but increased expression of Wnt3a still occurred in fluoxetine-treated animals given K252a.
Gene_expression (expression) of Wnt3a associated with fluoxetine
15) Confidence 0.53 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0.06 Pain Relevance 0.62
The first experiment determines whether intracerebroventricular (icv) infusion of the Trk antagonist K252a, can block the action of fluoxetine (10 mg/kg/day) on progenitor cell mitosis, and the effect it has on the expression of pCREB and Wnt3a in the granular neurons in the dentate gyrus.
Gene_expression (expression) of Wnt3a in dentate gyrus associated with antagonist, intracerebroventricular and fluoxetine
16) Confidence 0.53 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0.23 Pain Relevance 0.59
The second explores whether rats treated with the same dose of fluoxetine for 7 days show any change in progenitor proliferation, or expression of pCREB or Wnt3a, compared to those treated for 14 days.
Gene_expression (expression) of Wnt3a associated with fluoxetine
17) Confidence 0.53 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2965105 Disease Relevance 0.21 Pain Relevance 0.57

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