INT340809
From wiki-pain
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Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
To confirm whether BTLA takes part in the immunopathogenesis of HSK in BALB/c mice, we first tested the expression of BTLA in the cornea and peripheral blood of HSV-1 infected mice. | |||||||||||||||
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However, BTLA expression did not increase in CD8+ T cells after HSV-1 infection (Figure 1C). | |||||||||||||||
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In the normal uninfected mouse cornea, BTLA expression was only detected in the corneal epithelium (Figure 1D). | |||||||||||||||
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Immunohistochemistry results for BTLA expression in the corneas are shown in Figure 1D. | |||||||||||||||
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Immunohistochemical detection of BTLA expression in murine corneas after HSV-1 infection | |||||||||||||||
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As shown in Figure 3A, at 48 h post-transfection, the immunofluorescence intensity was high in HEK293 cells transfected with pBTLA. | |||||||||||||||
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BTLA expression is upregulated in the murine peripheral blood and cornea after HSV-1 infection | |||||||||||||||
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The restriction endonuclease digestion and sequence analyses confirmed successful construction of the recombinant expression plasmid pcDNA-BTLA (named pBTLA).
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The results indicate that the target protein BTLA was expressed successfully.
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The recombinant pBTLA was transfected into the HEK293 cell line (the Institute of Genetics of Fudan University, Shanghai, China) to express the target protein BTLA, which was detected by indirect immunofluorescence and western blotting. | |||||||||||||||
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A representative plot gated on CD4+ T cells demonstrated higher BTLA expression (20.14%) on day 10 after corneal inoculation with HSV-1 compared to day 0 (3.10%, Figure 1B). | |||||||||||||||
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The restriction endonuclease digestion and sequence analyses confirmed successful construction of the recombinant expression plasmid pcDNA-BTLA (named pBTLA).
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BTLA expression increased in CD4+ T cells, peaking on day 10 after infection (Figure 1A). | |||||||||||||||
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However, in corneal tissues derived from HSV-1 infected mice, BTLA expression was increased in the corneal epithelium, stroma, and endothelium (Figure 1E). | |||||||||||||||
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Forty-eight hours after transfection, the cells were fixed with 100% acetone at 20 °C for 15 min and BTLA expression was detected using the indirect immunofluorescence assay, with a monoclonal antibody against the BTLA protein and a FITC-labeled rabbit-anti-mouse IgG (Sigma, St. | |||||||||||||||
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The recombinant pBTLA was transfected into the HEK293 cell line (the Institute of Genetics of Fudan University, Shanghai, China) to express the target protein BTLA, which was detected by indirect immunofluorescence and western blotting. | |||||||||||||||
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Expression level of the recombinant plasmid pBTLA | |||||||||||||||
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In addition, the stroma of infected cornea contained many inflammatory cells that expressed BTLA (Figure 1E). | |||||||||||||||
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l connection system was obtained with the BTLA gene and pcDNA3.1 (BTLA:pcDNA3.1=1:1): pcDNA3.1 segment (4 ? | |||||||||||||||
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BTLA expression in the infected cornea was detected by immunohistochemistry. | |||||||||||||||
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General Comments
This test has worked.