INT340809

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Context Info
Confidence 0.77
First Reported 2010
Last Reported 2010
Negated 0
Speculated 1
Reported most in Body
Documents 1
Total Number 41
Disease Relevance 27.14
Pain Relevance 1.69

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
T cells 7
cornea 6
blood 5
stroma 2
corneal epithelium 2
Btla (Mus musculus)
Pain Link Frequency Relevance Heat
Inflammation 615 99.76 Very High Very High Very High
imagery 41 98.04 Very High Very High Very High
cytokine 328 93.12 High High
Inflammatory response 41 77.76 Quite High
cINOD 41 7.04 Low Low
corticosteroid 41 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Infection 1599 99.92 Very High Very High Very High
INFLAMMATION 697 99.76 Very High Very High Very High
Cold Sores 2173 99.64 Very High Very High Very High
Keratitis 1517 99.26 Very High Very High Very High
Sprains And Strains 246 98.92 Very High Very High Very High
Herpes Simplex Virus 123 97.32 Very High Very High Very High
Disease 328 96.96 Very High Very High Very High
Eye Disease 82 96.24 Very High Very High Very High
Herpes Simplex Virus Infection 123 93.72 High High
Necrosis 41 93.32 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
To confirm whether BTLA takes part in the immunopathogenesis of HSK in BALB/c mice, we first tested the expression of BTLA in the cornea and peripheral blood of HSV-1 infected mice.
Gene_expression (expression) of BTLA in blood associated with cold sores and keratitis
1) Confidence 0.77 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0.87 Pain Relevance 0.04
However, BTLA expression did not increase in CD8+ T cells after HSV-1 infection (Figure 1C).
Gene_expression (expression) of BTLA in T cells associated with cold sores and infection
2) Confidence 0.77 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 1.06 Pain Relevance 0.07
In the normal uninfected mouse cornea, BTLA expression was only detected in the corneal epithelium (Figure 1D).
Gene_expression (expression) of BTLA in corneal epithelium
3) Confidence 0.77 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 1.03 Pain Relevance 0.07
Immunohistochemistry results for BTLA expression in the corneas are shown in Figure 1D.
Gene_expression (expression) of BTLA in corneas
4) Confidence 0.77 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 1.05 Pain Relevance 0.07
Immunohistochemical detection of BTLA expression in murine corneas after HSV-1 infection
Gene_expression (expression) of BTLA in corneas associated with cold sores and infection
5) Confidence 0.77 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0.29 Pain Relevance 0
As shown in Figure 3A, at 48 h post-transfection, the immunofluorescence intensity was high in HEK293 cells transfected with pBTLA.
Gene_expression (transfected) of pBTLA
6) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0.49 Pain Relevance 0.04
BTLA expression is upregulated in the murine peripheral blood and cornea after HSV-1 infection
Gene_expression (expression) of BTLA in blood associated with cold sores and infection
7) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0.79 Pain Relevance 0.05
The restriction endonuclease digestion and sequence analyses confirmed successful construction of the recombinant expression plasmid pcDNA-BTLA (named pBTLA).


Gene_expression (expression) of pBTLA
8) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0.46 Pain Relevance 0.04
The results indicate that the target protein BTLA was expressed successfully.


Gene_expression (expressed) of BTLA
9) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0.65 Pain Relevance 0.09
The recombinant pBTLA was transfected into the HEK293 cell line (the Institute of Genetics of Fudan University, Shanghai, China) to express the target protein BTLA, which was detected by indirect immunofluorescence and western blotting.
Gene_expression (express) of BTLA
10) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0 Pain Relevance 0
A representative plot gated on CD4+ T cells demonstrated higher BTLA expression (20.14%) on day 10 after corneal inoculation with HSV-1 compared to day 0 (3.10%, Figure 1B).
Gene_expression (expression) of BTLA in T cells associated with cold sores
11) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 1.00 Pain Relevance 0.06
The restriction endonuclease digestion and sequence analyses confirmed successful construction of the recombinant expression plasmid pcDNA-BTLA (named pBTLA).


Gene_expression (expression) of pcDNA-BTLA
12) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0.46 Pain Relevance 0.04
BTLA expression increased in CD4+ T cells, peaking on day 10 after infection (Figure 1A).
Gene_expression (expression) of BTLA in T cells associated with infection
13) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0.93 Pain Relevance 0.04
However, in corneal tissues derived from HSV-1 infected mice, BTLA expression was increased in the corneal epithelium, stroma, and endothelium (Figure 1E).
Gene_expression (expression) of BTLA in corneal epithelium associated with cold sores
14) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 1.01 Pain Relevance 0.07
Forty-eight hours after transfection, the cells were fixed with 100% acetone at –20 °C for 15 min and BTLA expression was detected using the indirect immunofluorescence assay, with a monoclonal antibody against the BTLA protein and a FITC-labeled rabbit-anti-mouse IgG (Sigma, St.
Gene_expression (expression) of BTLA
15) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0 Pain Relevance 0
The recombinant pBTLA was transfected into the HEK293 cell line (the Institute of Genetics of Fudan University, Shanghai, China) to express the target protein BTLA, which was detected by indirect immunofluorescence and western blotting.
Gene_expression (transfected) of pBTLA
16) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0 Pain Relevance 0
Expression level of the recombinant plasmid pBTLA
Gene_expression (Expression) of pBTLA
17) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0.45 Pain Relevance 0.04
In addition, the stroma of infected cornea contained many inflammatory cells that expressed BTLA (Figure 1E).
Gene_expression (expressed) of BTLA in stroma associated with inflammation
18) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0.90 Pain Relevance 0.05
l connection system was obtained with the BTLA gene and pcDNA3.1 (BTLA:pcDNA3.1=1:1): pcDNA3.1 segment (4 ?
Gene_expression (gene) of BTLA
19) Confidence 0.67 Published 2010 Journal Molecular Vision Section Body Doc Link PMC2965573 Disease Relevance 0 Pain Relevance 0
BTLA expression in the infected cornea was detected by immunohistochemistry.
Gene_expression (expression) of BTLA in cornea
20) Confidence 0.60 Published 2010 Journal Molecular Vision Section Abstract Doc Link PMC2965573 Disease Relevance 1.20 Pain Relevance 0.03

General Comments

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