INT343069

Context Info Confidence 0.56 First Reported 2010 Last Reported 2010 Negated 1 Speculated 1 Reported most in Body Documents 1 Total Number 20 Disease Relevance 0.23 Pain Relevance 0.16

cellular_component (Pld4) biological_process (Pld4)

Anatomy Link Frequency brain 5 spleen 5 corpus callosum 1 cerebellum 1 microglial cells 1 Pld4 (Mus musculus)

Pain Link Frequency Relevance Heat cerebral cortex 20 92.08 anesthesia 20 5.00 Kinase C 20 5.00 Neurotransmitter 20 5.00 imagery 20 5.00

Disease Link Frequency Relevance Heat Pox Virus Infection 40 85.60 Hypersensitivity 60 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

PLD4 mRNA expression was also distinguishable from glial fibrillary acidic protein (GFAP) mRNA expression in astrocytes (Fig. 4B-c, c', d and d'), neural stem cells, and radial glia in the SVZ (Fig. 4-d and d').

Transcription (expression) of PLD4 in astrocytes

1) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

These results demonstrated that PLD4 mRNA was expressed in microglial cells in early postnatal mouse brains.

Transcription (expressed) of PLD4 in microglial cells

2) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0.05

In non-neuronal tissues, PLD4 mRNA expression was widespread, but predominantly distributed in the spleen.

Transcription (expression) of PLD4 in spleen

3) Confidence 0.56 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

To identify cell types that express PLD4 mRNA, double labeling was performed by ISH and immunohistochemistry (IHC), respectively, on cerebellar sections of P7 mice (Fig. 5).

Transcription (express) of PLD4

4) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0.03

These results demonstrated that PLD4 mRNA is expressed in cell type(s) preferentially localized around white matter and throughout brain regions during the early postnatal period.

Transcription (expressed) of PLD4 in brain

5) Confidence 0.56 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

The present study identified the characteristic mRNA expression profile of PLD4 during mouse brain development.

Transcription (profile) of PLD4 in brain

6) Confidence 0.54 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

Among the non-neuronal tissues tested, PLD4 mRNA levels were greatest in the spleen, and intense PLD4 expression was detected around the splenic marginal zone, where macrophages are located [35].

Transcription (levels) of PLD4 in spleen

7) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

Temporal expression analysis by GeneChip microarrays revealed low levels of PLD4 mRNA expression during postnatal development of the mouse cerebellum (Fig. 2).

Transcription (expression) of PLD4 in cerebellum

8) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0.08 Pain Relevance 0

PLD4 mRNA expression is prominent during the first postnatal week in the mouse cerebellum and exhibits greatest expression in the mouse spleen

Transcription (expression) of PLD4 in spleen

9) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0.09 Pain Relevance 0

PLD4 mRNA expression was distinguishable from myelin basic protein (MBP) mRNA expression in oligodendrocytes clustered within the cerebellar white matter (Fig. 4B-b and b') and CC (Fig. 4B-e and e').

Transcription (expression) of PLD4 in oligodendrocytes

10) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

The mouse spleen expressed the highest levels of PLD4 mRNA among the different tissues tested, as shown in Figure 3.

Transcription (levels) of PLD4 in spleen

11) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0.04

PLD4 mRNA expression is restricted to a subset of cells located within the white matter region during the early postnatal stage

Transcription (expression) of PLD4 in white matter

12) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

At P21, detectable signals for PLD4 mRNA were not identified in any brain regions using these methods (Fig. 4A-b).

Neg (not) Transcription (identified) of PLD4 in brain

13) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

In contrast, temporal and cellular expression patterns of PLD4 mRNA are fairly restricted to developing mouse brains and various tissues.

Transcription (patterns) of PLD4 in brains

14) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

The PLD4 mRNA expression in mouse brains and non-neuronal tissues were analyzed by using transcriptome database CDT-DB.

Transcription (expression) of PLD4 in neuronal

15) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

PLD4 mRNA expression gradually increased after birth, peaked at P7, and was significantly reduced thereafter (Fig.

Transcription (expression) of PLD4

16) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0.07 Pain Relevance 0

Results demonstrated low to modest, but characteristic, PLD4 mRNA expression in a subset of cells preferentially localized around white matter regions, including the corpus callosum and cerebellar white matter, during the first postnatal week.

Transcription (expression) of PLD4 in cerebellar white matter

17) Confidence 0.42 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

Cellular expression of PLD4 mRNA in P7 and P21 mouse brain sections was analyzed by in situ hybridization (ISH) (Fig. 4).

Spec (analyzed) Transcription (expression) of PLD4 in brain

18) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

The mouse spleen expressed the highest levels of PLD4 mRNA among the different tissues tested, as shown in Figure 3.

Transcription (expressed) of PLD4 in spleen

19) Confidence 0.42 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0.04

Results demonstrated low to modest, but characteristic, PLD4 mRNA expression in a subset of cells preferentially localized around white matter regions, including the corpus callosum and cerebellar white matter, during the first postnatal week.

Transcription (expression) of PLD4 in corpus callosum

20) Confidence 0.14 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2978679 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.