INT351538

Context Info Confidence 0.38 First Reported 2010 Last Reported 2011 Negated 0 Speculated 0 Reported most in Body Documents 8 Total Number 8 Disease Relevance 4.44 Pain Relevance 0.49

extracellular space (Prom1) plasma membrane (Prom1) cilium (Prom1)

Anatomy Link Frequency stem cells 2 macrophages 1 Prom1 (Mus musculus)

Pain Link Frequency Relevance Heat imagery 224 99.80 Central nervous system 8 63.72 anesthesia 8 5.00 neurotrophin 3 8 5.00 Glutamate 7 5.00 isoflurane 7 5.00 long-term potentiation 1 5.00 cerebral cortex 1 5.00

Disease Link Frequency Relevance Heat Cancer 562 99.98 Viral Infection 13 96.00 Cytomegalovirus Infection 56 93.00 Infection 48 87.36 Brain Tumor 7 86.00 Glioblastoma 70 84.16 Ependymoma 7 83.04 Malignant Neoplastic Disease 21 83.00 Medulloblastoma 7 81.84 Calcification 1 79.56

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

As shown in Figure 4D, a significant population of CD11b-positive cells had indeed bound injected AC133.1 (double-positive population in the left and right panel of Figure 4D).

AC133 Binding (bound) of

1) Confidence 0.38 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3004948 Disease Relevance 0.15 Pain Relevance 0

Taken together, our data provide first evidence that noninvasive in vivo imaging of tumor-associated CD133 (AC133) is feasible.

CD133 Binding (associated) of associated with cancer and imagery

2) Confidence 0.34 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3004948 Disease Relevance 0.26 Pain Relevance 0.13

Taken together, our data provide first evidence that noninvasive in vivo imaging of tumor-associated CD133 (AC133) is feasible.

AC133 Binding (associated) of associated with cancer and imagery

3) Confidence 0.34 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3004948 Disease Relevance 0.26 Pain Relevance 0.13

In the digested cell mix derived from the HCT116 wild-type xenografts, macrophages with bound AC133 antibody constituted less than 10% of antibody-bearing cells.

AC133 Binding (bound) of in macrophages

4) Confidence 0.33 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3004948 Disease Relevance 0.63 Pain Relevance 0.13

We also found that a small population of CD133-negative cells in the tumors bound injected AC133.1-Alexa488.

AC133 Binding (bound) of associated with cancer

5) Confidence 0.33 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3004948 Disease Relevance 0.24 Pain Relevance 0

Previously performed control experiments confirmed that the treatment of HCT116 cells with accutase and blendzyme, the enzymes used for preparing single-cell suspensions, did not remove cell-surface CD133 nor bound AC133.1-Alexa488 antibodies (Figure 4A).

AC133 Binding (bound) of

6) Confidence 0.33 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3004948 Disease Relevance 0.42 Pain Relevance 0.03

Stem cells are characterized by a set of glycosylation-dependent epitopes in the extracellular portion of CD133 recognized by the mAb AC133 (CD133/1) and the mAbs AC141 and 293C (CD133/2).

AC133 Binding (recognized) of in Stem cells

7) Confidence 0.30 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3004948 Disease Relevance 0.99 Pain Relevance 0

There is an abundance of NSCs in the fetal brain, in this study we will use the term neural stem cells to refer to all classes of immature and proliferating cells that reacted with CD133 and nestin.

CD133 Binding (reacted) of in stem cells

8) Confidence 0.19 Published 2011 Journal PLoS ONE Section Body Doc Link PMC3020957 Disease Relevance 1.48 Pain Relevance 0.06

General Comments

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