INT353427

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Context Info
Confidence 0.76
First Reported 2010
Last Reported 2010
Negated 0
Speculated 1
Reported most in Body
Documents 1
Total Number 25
Disease Relevance 7.47
Pain Relevance 0

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Golgi apparatus (Rnd3) intracellular (Rnd3)
Anatomy Link Frequency
fibroblasts 4
Rnd3 (Mus musculus)
Pain Link Frequency Relevance Heat
anesthesia 25 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Cancer 925 99.84 Very High Very High Very High
Sarcoma 350 99.60 Very High Very High Very High
Breast Cancer 25 99.48 Very High Very High Very High
Skin Cancer 75 98.32 Very High Very High Very High
Reprotox - General 1 25 98.32 Very High Very High Very High
Malignant Neoplastic Disease 125 89.60 High High
Fibrosarcoma 25 83.92 Quite High
Stress 50 75.20 Quite High
Metastasis 400 71.60 Quite High
Severe Combined Immunodeficiency 100 62.88 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Our observation that ectopic Rnd3 expression in phase II and III tumorigenic cen3tel cells reduced their invasive capacity points to Rnd3 as a possible regulator of invasion of cells of mesenchymal origin.
Gene_expression (expression) of Rnd3
1) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.28 Pain Relevance 0
In the clones stably expressing Rnd3, we found no major changes in cellular morphology compared to parental cells, or actin depolymerization (not shown).
Gene_expression (expressing) of Rnd3
2) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0 Pain Relevance 0
The increased invasiveness of tumorigenic cells was associated with reduced expression of Rnd3 (also known as RhoE), a cellular inhibitor of ROCK.
Gene_expression (expression) of Rnd3
3) Confidence 0.76 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC3014295 Disease Relevance 0.33 Pain Relevance 0
Rnd3 overexpression induces actin fiber disassembly in several cell types [34].
Gene_expression (overexpression) of Rnd3
4) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0 Pain Relevance 0
We then investigated whether exogenous Rnd3 expression reduced the in vivo metastatic potential of phase III tumorigenic cen3tel cells.
Spec (whether) Gene_expression (expression) of Rnd3
5) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.19 Pain Relevance 0
Rnd3 shows reduced expression in prostate cancer and seems to have a protective role against breast cancer [25]; however, increased Rnd3 expression has been found in other epithelial tumors and in metastatic melanoma cells [42], suggesting that the genetic background of cancer cells can influence the role of Rnd3 in trasformation.
Gene_expression (expression) of Rnd3 associated with cancer, breast cancer, skin cancer and reprotox - general 1
6) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.81 Pain Relevance 0
Pinner and Sahai [41] showed that a fine balance between PDK1 and Rnd3 expression is required for the amoeboid movement of melanoma cells, because the two proteins have opposing roles in controlling ROCK-I activity.
Gene_expression (expression) of Rnd3 associated with skin cancer
7) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.28 Pain Relevance 0
In fact, it has been shown that in mouse 3T3 fibroblasts, the strong effects induced by acute Rnd3 expression on the cytoskeleton are only transient and disappear within hours, even though the levels of the protein are still high [35].
Gene_expression (expression) of Rnd3 in fibroblasts
8) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0 Pain Relevance 0
Rnd3 expression inversely correlates with the invasive and metastatic properties of tumorigenic cen3tel cell
Gene_expression (expression) of Rnd3
9) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0 Pain Relevance 0
In the Rnd3 expressing clones we did find lower level of p-MLC than in parental and mock-transfected cells, which could be due to an Rnd3-induced reduction in ROCK kinase activity (Fig. 5F).
Gene_expression (expressing) of Rnd3
10) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0 Pain Relevance 0
We analyzed the metastatic potential of Rnd3 clone 16, because of the greater stability of Rnd3 expression in this clone compared to that in clone 8 (not shown).
Gene_expression (expression) of Rnd3
11) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.35 Pain Relevance 0
Rnd3 expressing clones showed proliferation rates similar to that of parental and mock transfected cells (data not shown).
Gene_expression (expressing) of Rnd3
12) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0 Pain Relevance 0
Our results indicate that reduced Rnd3 expression in tumor cells of mesenchymal origin can be linked to the acquisition of a ROCK-dependent mode of invasion and can increase the metastatic potential.
Gene_expression (expression) of Rnd3 associated with cancer
13) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.87 Pain Relevance 0
We analyzed invasion in the five Rnd3 expressing clones and, in all of them, it was lower than in mock transfected cells (Fig. 5G), indicating that Rnd3 expression can modulate invasion of neoplastically transformed human fibroblasts.
Gene_expression (expression) of Rnd3 in fibroblasts
14) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.11 Pain Relevance 0
Thus, analysis of Rnd3 expression in human sarcomas might give information on the mechanism of invasion adopted by the tumor cells and help orientate the therapeutic strategy [37].
Gene_expression (expression) of Rnd3 associated with sarcoma and cancer
15) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.97 Pain Relevance 0
Very little is known about Rnd3 expression in human sarcomas.
Gene_expression (expression) of Rnd3 associated with sarcoma
16) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.86 Pain Relevance 0
To test whether Rnd3 levels affected tumorigenic cen3tel cell invasiveness, we transfected phase II and phase III tumorigenic cells with an EGFP-Rnd3-expression vector and isolated clones with exogenous Rnd3 expression, three from phase II tumorigenic cen3tel cells and two from phase III cells (Fig. 5C).
Gene_expression (expression) of Rnd3
17) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0 Pain Relevance 0
Gadea et al. [14] showed that overexpression of Rnd3 in mouse embryonic fibroblasts in which amoeboid migration had been induced by p53 knockdown led to reduced invasion.
Gene_expression (overexpression) of Rnd3 in fibroblasts
18) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.25 Pain Relevance 0
By microarray analysis and western blotting we did not observe any evident change in ROCK or RhoA expression in tumorigenic cen3tel cells (data not shown); however, microarray and western blot analyses revealed that the cellular inhibitor of ROCK-I, Rnd3 (Riento et al. 2003), was expressed at lower levels in tumorigenic cen3tel cells compared to non-tumorigenic ones (Fig. 5A, B), suggesting that Rnd3 downregulation might play a role in the transition to the ROCK dependent mode of invasion.
Gene_expression (expressed) of Rnd3
19) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0 Pain Relevance 0
Finally, the low metastatic potential observed in phase III tumorigenic cen3tel cells that ectopically express Rnd3 suggests that Rnd3 exerts its effect also in vivo and that its downregulation can have a pro-metastatic effect.
Gene_expression (exerts) of Rnd3
20) Confidence 0.66 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3014295 Disease Relevance 0.64 Pain Relevance 0

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