INT61158
From wiki-pain
|
|
|
|
|
Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Total CREB and phosphorylated CREB in both ipsilateral and contralateral dorsal horn neurons were dramatically reduced in antisense ODN injected PSNL rats 1 week after injection. | |||||||||||||||
| |||||||||||||||
|
The extent of reduction of total CREB and phosphorylated CREB containing cells in the dorsal horn ipsilateral to injury was greater than in the contralateral dorsal horn. | |||||||||||||||
| |||||||||||||||
|
The extent of reduction of total CREB and phosphorylated CREB containing cells in the dorsal horn ipsilateral to injury was greater than in the contralateral dorsal horn. | |||||||||||||||
| |||||||||||||||
|
It was found that the antisense oligonucleotide-induced reduction in CREB levels mimicked the effect of morphine on certain, but not all, cAMP pathway proteins in this brain region, whereas a large number of other signal transduction proteins tested were unaffected by this treatment. | |||||||||||||||
| |||||||||||||||
|
The functional significance of this CREB down-regulation was then investigated by the use of an anti-sense oligonucleotide strategy that produces a specific and sustained decrease in CREB levels in the NAc, without detectable toxicity. | |||||||||||||||
| |||||||||||||||
|
Stressed rats presented a decrease in CAM-L1, laminin, and pCREB, specifically in brain areas implicated in stress. | |||||||||||||||
| |||||||||||||||
|
A significant decrease in both phospho-CREB at Ser133 and phospho-DARPP-32 at Thr34 in the rat caudate putamen was produced by cocaine, GBR 12909, fluoxetine or nisoxetine. | |||||||||||||||
| |||||||||||||||
|
Under these conditions, we found that P-CREB labeling was decreased by 90% (data not shown). | |||||||||||||||
| |||||||||||||||
|
Total CREB and phosphorylated CREB in both ipsilateral and contralateral dorsal horn neurons were dramatically reduced in antisense ODN injected PSNL rats 1 week after injection. | |||||||||||||||
| |||||||||||||||
|
A further link between NMDA receptor activation, CREB phosphorylation, and Müller cell proliferation was obtained by cotreatment of the cells with NMDA and the CREB inhibitor for 24 h and coimmunolabeling for BrdU and P-CREB. | |||||||||||||||
| |||||||||||||||
|
Our results show that the number of proliferating cells decreases in CREB-inhibitor-treated cells as compared to control cultures (Figure 4A-C). | |||||||||||||||
| |||||||||||||||
|
Knockdown of CREB by small interfering RNA or a dominant-negative form of CREB diminished acid-elevated CGRP expression. | |||||||||||||||
| |||||||||||||||
|
Treatment of SNL rats with alpha5-antisense significantly reduced pCREB immunoreactivity. | |||||||||||||||
| |||||||||||||||
|
Intrathecal clonidine decreased pCREB immunoreactive cells in the deep dorsal horn of injured animals. | |||||||||||||||
| |||||||||||||||
|
However, calphostin C (a PKC inhibitor) and or SB203580 (a p38 inhibitor) partially but significantly reduced C2 ceramide-induced proENK mRNA expression as well as phospho-CREB level. | |||||||||||||||
| |||||||||||||||
|
Antisense-mediated knockdown of CREB (cyclic AMP response-element binding proteins) has however no effect. | |||||||||||||||
| |||||||||||||||
|
As in experiment 1, pCREB was reduced in fluoxetine and K252a-treated animals, but increased expression of Wnt3a still occurred in fluoxetine-treated animals given K252a. | |||||||||||||||
| |||||||||||||||
|
Caspace 1, D2 dopamine receptor, GABA-A alpha1 subunit, GRIA 1/3/4, Galphai2, PSD-95 and CREB were down-regulated in the NAc shell with morphine administration. | |||||||||||||||
| |||||||||||||||
|
A series of control experiments with various [K+] and stimulus durations revealed that a 45-s delay in control solution provides maximal phosphorylation of CREB; beyond this time, pCREB levels decline, reaching baseline levels within a few minutes. | |||||||||||||||
| |||||||||||||||
|
Indeed, Dowlatshahi et al. [89] reported that CREB levels are decreased in the cerebral cortex of depressed patients. | |||||||||||||||
| |||||||||||||||
|
General Comments
This test has worked.