INT63852
From wiki-pain
|
|
|
|
|
Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Modulation of glycine receptor subunits and gephyrin expression in the rat facial nucleus after axotomy. | |||||||||||||||
| |||||||||||||||
|
Thus, synapsin-1 labeling distinctly revealed large clusters present at extrasynaptic sites, whereas co-transfection of EGFP-Homer1c and mCherry-gephyrin indicated that synaptic ? | |||||||||||||||
| |||||||||||||||
|
5 cells; pVIAAT/Gephyrin/Homer1c? | |||||||||||||||
| |||||||||||||||
|
5 cells; pVIAAT/Gephyrin/Homer1c? | |||||||||||||||
| |||||||||||||||
|
We used co-expression of mCherry-Homer1c and EGFP-gephyrin to distinguish glutamatergic and GABAergic postsynaptic sites and ensure that all markers analyzed were present in the same cell. | |||||||||||||||
| |||||||||||||||
|
Mismatched synapses were present but in a significantly smaller proportion than matched synapses (22.7±4.4% gephyrin clusters apposed to vGlut1; 16.5±3.5% Homer1c clusters apposed to VIAAT; nvGlut1? | |||||||||||||||
| |||||||||||||||
|
We report here the effect of axotomy on the expression of glycine receptors, gephyrin, vesicular inhibitory amino acid transporter (VIAAT) and synapsin I in rat facial motor neurons as demonstrated by in situ hybridization and immunohistochemistry. | |||||||||||||||
| |||||||||||||||
|
Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed that the glycine receptor alpha1, alpha2, beta subunits and gephyrin mRNAs were expressed at comparable levels in strychnine-treated and untreated cultures. | |||||||||||||||
| |||||||||||||||
|
The number of immunoreactive cells and the subcellular distribution of gephyrin and GlyR subunits was determined with standard and confocal immunofluorescence. | |||||||||||||||
| |||||||||||||||
|
The effects of chronic strychnine treatment on the expression and cellular distribution of gephyrin and glycine receptor were therefore tested using primary cultures of spinal cord neurons. | |||||||||||||||
| |||||||||||||||
|
Chhatwal et al. [100] showed that gephyrin mRNA and protein levels in the BLA significantly | |||||||||||||||
| |||||||||||||||
|
modulation of gephyrin and GABAA receptor expression | |||||||||||||||
| |||||||||||||||
|
The proportion of gephyrin and glycine receptor-immunoreactive (IR) cells was unaffected by strychnine treatment. | |||||||||||||||
| |||||||||||||||
|
Protein kinase C gamma isoform (PKCgamma) is present at high levels in the spinal and medullary dorsal horns and is thought to play a role in the sensitization of dorsal horn neurons in certain pain states. | |||||||||||||||
| |||||||||||||||
|
Neural influence on protein kinase C isoform expression in skeletal muscle. | |||||||||||||||
| |||||||||||||||
|
To explore further how nerve regulates PKC expression, we characterized PKC isoform expression in rat myotubes deprived of neural input. | |||||||||||||||
| |||||||||||||||
|
They are characterized by expression of the gamma isoform of protein kinase C (PKC?) | |||||||||||||||
| |||||||||||||||
|
We anatomically show that the activation was mediated through a local circuit involving neurons expressing the gamma isoform of protein kinase C (PKC?). | |||||||||||||||
| |||||||||||||||
|
Differences in D1-like receptor regulation of diuresis, natriuresis, Na+-K+-ATPase activity, and rat renal protein kinase C isoform expression between normotensive and hypertensive rats suggest that renal D1-like receptor dysfunction may be the mechanism responsible for generation of hypertensive rats [78]. | |||||||||||||||
| |||||||||||||||
|
The relative increased proportion of fibers expressing the MHCslow isoform may explain the greater fatigue resistance of diabetic diaphragm [30]. | |||||||||||||||
| |||||||||||||||
|
General Comments
This test has worked.