INT63852

Context Info Confidence 0.63 First Reported 1996 Last Reported 2010 Negated 1 Speculated 1 Reported most in Body Documents 21 Total Number 26 Disease Relevance 5.64 Pain Relevance 8.20

protein targeting (Gphn) plasma membrane (Gphn) cytoskeleton (Gphn) intracellular (Gphn) protein binding, bridging (Gphn) cytoplasm (Gphn)

Anatomy Link Frequency neurons 5 inner part 1 diaphragm 1 skeletal muscle 1 nucleus 1 Gphn (Rattus norvegicus)

Pain Link Frequency Relevance Heat Kinase C 33 100.00 amygdala 124 99.68 Neuropathic pain 20 99.00 Pain 47 98.86 GABAergic 196 98.64 Dorsal horn neuron 14 98.54 Peripheral nerve injury 3 98.08 Dorsal horn 115 97.88 substantia gelatinosa 5 97.68 Spinal cord 18 97.20

Disease Link Frequency Relevance Heat Neuropathic Pain 115 99.00 Fatigue 17 99.00 Hypertension 9 98.96 Pain 53 98.86 Natriuresis 2 98.86 Diuresis 2 98.60 Nociception 83 98.12 Diabetes Mellitus 63 98.12 Anxiety Disorder 244 97.76 Nervous System Injury 9 97.64

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Modulation of glycine receptor subunits and gephyrin expression in the rat facial nucleus after axotomy.

Gene_expression (expression) of gephyrin in nucleus

1) Confidence 0.63 Published 2005 Journal Eur. J. Neurosci. Section Title Doc Link 15733085 Disease Relevance 0 Pain Relevance 0.07

Thus, synapsin-1 labeling distinctly revealed large clusters present at extrasynaptic sites, whereas co-transfection of EGFP-Homer1c and mCherry-gephyrin indicated that “synaptic” ?

Gene_expression (co-transfection) of mCherry-gephyrin

2) Confidence 0.52 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2901346 Disease Relevance 0 Pain Relevance 0.23

5 cells; pVIAAT/Gephyrin/Homer1c?

Gene_expression (/) of Gephyrin

3) Confidence 0.52 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2901346 Disease Relevance 0 Pain Relevance 0.09

5 cells; pVIAAT/Gephyrin/Homer1c?

Gene_expression (/) of Gephyrin

4) Confidence 0.52 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2901346 Disease Relevance 0 Pain Relevance 0.09

We used co-expression of mCherry-Homer1c and EGFP-gephyrin to distinguish glutamatergic and GABAergic postsynaptic sites and ensure that all markers analyzed were present in the same cell.

Gene_expression (expression) of gephyrin associated with gabaergic

5) Confidence 0.52 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2901346 Disease Relevance 0 Pain Relevance 0.17

Mismatched synapses were present but in a significantly smaller proportion than matched synapses (22.7±4.4% gephyrin clusters apposed to vGlut1; 16.5±3.5% Homer1c clusters apposed to VIAAT; nvGlut1?

Gene_expression (clusters) of gephyrin in synapses

6) Confidence 0.45 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2901346 Disease Relevance 0 Pain Relevance 0.08

We report here the effect of axotomy on the expression of glycine receptors, gephyrin, vesicular inhibitory amino acid transporter (VIAAT) and synapsin I in rat facial motor neurons as demonstrated by in situ hybridization and immunohistochemistry.

Gene_expression (expression) of gephyrin in motor neurons

7) Confidence 0.42 Published 2005 Journal Eur. J. Neurosci. Section Abstract Doc Link 15733085 Disease Relevance 0 Pain Relevance 0.07

Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis revealed that the glycine receptor alpha1, alpha2, beta subunits and gephyrin mRNAs were expressed at comparable levels in strychnine-treated and untreated cultures.

Gene_expression (expressed) of gephyrin

8) Confidence 0.25 Published 1998 Journal J. Cell. Sci. Section Abstract Doc Link 9427682 Disease Relevance 0 Pain Relevance 0.09

The number of immunoreactive cells and the subcellular distribution of gephyrin and GlyR subunits was determined with standard and confocal immunofluorescence.

Gene_expression (distribution) of gephyrin

9) Confidence 0.25 Published 1998 Journal J. Cell. Sci. Section Abstract Doc Link 9427682 Disease Relevance 0 Pain Relevance 0.08

The effects of chronic strychnine treatment on the expression and cellular distribution of gephyrin and glycine receptor were therefore tested using primary cultures of spinal cord neurons.

Gene_expression (expression) of gephyrin in spinal cord neurons associated with spinal cord

10) Confidence 0.25 Published 1998 Journal J. Cell. Sci. Section Abstract Doc Link 9427682 Disease Relevance 0 Pain Relevance 0.09

Chhatwal et al. [100] showed that gephyrin mRNA and protein levels in the BLA significantly

Gene_expression (levels) of gephyrin associated with amygdala

11) Confidence 0.23 Published 2007 Journal Neural Plasticity Section Body Doc Link PMC1838961 Disease Relevance 0.19 Pain Relevance 0.55

modulation of gephyrin and GABAA receptor expression

Gene_expression (expression) of gephyrin

12) Confidence 0.23 Published 2007 Journal Neural Plasticity Section Body Doc Link PMC1838961 Disease Relevance 0.18 Pain Relevance 0.51

The proportion of gephyrin and glycine receptor-immunoreactive (IR) cells was unaffected by strychnine treatment.

Gene_expression (proportion) of gephyrin

13) Confidence 0.22 Published 1998 Journal J. Cell. Sci. Section Abstract Doc Link 9427682 Disease Relevance 0 Pain Relevance 0.07

Protein kinase C gamma isoform (PKCgamma) is present at high levels in the spinal and medullary dorsal horns and is thought to play a role in the sensitization of dorsal horn neurons in certain pain states.

Gene_expression (present) of isoform in spinal associated with pain, kinase c and dorsal horn neuron

14) Confidence 0.02 Published 2002 Journal Neurosignals Section Abstract Doc Link 12077482 Disease Relevance 0.09 Pain Relevance 0.33

Neural influence on protein kinase C isoform expression in skeletal muscle.

Gene_expression (expression) of isoform in skeletal muscle associated with kinase c

15) Confidence 0.02 Published 1996 Journal J. Neurosci. Section Title Doc Link 8756430 Disease Relevance 0.05 Pain Relevance 0.48

To explore further how nerve regulates PKC expression, we characterized PKC isoform expression in rat myotubes deprived of neural input.

Gene_expression (expression) of isoform in nerve associated with kinase c

16) Confidence 0.01 Published 1996 Journal J. Neurosci. Section Abstract Doc Link 8756430 Disease Relevance 0.13 Pain Relevance 0.45

They are characterized by expression of the gamma isoform of protein kinase C (PKC?)

Gene_expression (expression) of isoform associated with kinase c

17) Confidence 0.01 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2043493 Disease Relevance 0.41 Pain Relevance 0.35

We anatomically show that the activation was mediated through a local circuit involving neurons expressing the gamma isoform of protein kinase C (PKC?).

Gene_expression (expressing) of isoform in neurons associated with kinase c

18) Confidence 0.01 Published 2007 Journal PLoS ONE Section Abstract Doc Link PMC2043493 Disease Relevance 0.78 Pain Relevance 0.93

Differences in D1-like receptor regulation of diuresis, natriuresis, Na+-K+-ATPase activity, and rat renal protein kinase C isoform expression between normotensive and hypertensive rats suggest that renal D1-like receptor dysfunction may be the mechanism responsible for generation of hypertensive rats [78].

Gene_expression (expression) of isoform associated with natriuresis, diuresis, kinase c and hypertension

19) Confidence 0.01 Published 2007 Journal Current Neuropharmacology Section Body Doc Link PMC2644490 Disease Relevance 1.07 Pain Relevance 0.34

The relative increased proportion of fibers expressing the MHCslow isoform may explain the greater fatigue resistance of diabetic diaphragm [30].

Gene_expression (expressing) of MHCslow isoform in diaphragm associated with diabetes mellitus and fatigue

20) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2900215 Disease Relevance 0.48 Pain Relevance 0

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