INT65245
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Using epithelial HeLa cells expressing the endothelial NO synthase we show that ibuprofen-arginine releases NO and that this NO protects against the cytotoxic apoptogenic effects of staurosporine. | |||||||||||||||
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It has also been demonstrated that the molecular mechanism by which Waon therapy improves vascular flow and endothelial function involves increased expression of endothelial nitric oxide synthase (eNOS). | |||||||||||||||
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It has also been demonstrated that the molecular mechanism by which Waon therapy improves vascular flow and endothelial function involves increased expression of endothelial nitric oxide synthase (eNOS). | |||||||||||||||
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Our results revealed that the mRNA expression of inducible nitric oxide synthase (iNOS) was reduced by approximately 50% in GEB-pretreated mice versus the controls, whereas the mRNA expression levels of endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) remained unchanged. | |||||||||||||||
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Another interesting finding in the present study is the correlation between nNOS and eNOS expression in the placebo, but not in the ketorolac treatment group, which suggests an inhibitory effect of ketorolac on eNOS gene expression. | |||||||||||||||
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The down regulation of eNOS by NSAIDs, on the other hand, is in line with the suppression of eNOS expression by high-dose aspirin or meloxicam, in retinas of diabetic rats [36]. | |||||||||||||||
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Another interesting finding in the present study is the correlation between nNOS and eNOS expression in the placebo, but not in the ketorolac treatment group, which suggests an inhibitory effect of ketorolac on eNOS gene expression. | |||||||||||||||
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Meanwhile, Western blot showed that eNOS expression in the spinal cord of nNOS knockout mice was up-regulated compared with wild-type mice; immunohistochemical staining showed that the spinal eNOS was mainly distributed in superficial laminae of the dorsal horn. | |||||||||||||||
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Consistent with this observation, lung homogenates from female mice contained greater eNOS protein expression when compared to lung homogenates from male mice (Figure 3A). | |||||||||||||||
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Furthermore, in a mouse model of hindlimb ischemia, repeated Waon therapy increased eNOS protein expression, blood flow, and capillary density. | |||||||||||||||
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However, while the disruption of NOS1 gene did not alter the mRNA or protein levels of NOS2 in sham-operated mice a significant increase in the spinal cord expression of NOS3 was further demonstrated in sham-operated NOS1-KO mice [18]. | |||||||||||||||
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Expression of NOS3 in the spinal cord of WT, NOS2-KO and NOS1-KO mice | |||||||||||||||
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These results indicated that the compensatory changes in the spinal cord expression of NOS3 in NOS1-KO under basal conditions does not fully compensate for NOS1 function in neuropathic pain, which is mainly produced by NOS2. | |||||||||||||||
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Our results did not show significant changes in the expression of NOS2 and NOS3, mRNA and protein, on day 21th after sciatic nerve injury. | |||||||||||||||
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Sciatic nerve injury also increases the spinal cord expression of NOS1 and NOS2 isoforms, but not of NOS3, in WT and NOS1-KO mice respectively. | |||||||||||||||
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The possible alterations in NOS2 and NOS3 expression induced by neuropathic pain have been also evaluated in this study. | |||||||||||||||
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The mRNA and protein levels of NOS1, NOS2 and NOS3 in the spinal cord of WT and KO mice, at 21 days after surgery, were also assessed. | |||||||||||||||
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Our data also indicate that the enhanced spinal cord expression of NOS3 in NOS1-KO mice might compensates for the lack of NOS1 under basal conditions but not after sciatic nerve ligation. | |||||||||||||||
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Several works have demonstrated that nerve injury after sciatic nerve ligation evoked an increased NOS1 and NOS2, but not of NOS3, protein expression in the ipsilateral site of the dorsal root ganglia and the sciatic nerve [4], [6][11]. | |||||||||||||||
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In the present study, we also demonstrated that no compensatory changes in the spinal cord expression of NOS3 take place in sciatic nerve-injured NOS1 and NOS2 knockout mice as well as in sham-operated NOS2-KO mice [12]. | |||||||||||||||
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