INT65730

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Context Info
Confidence 0.78
First Reported 1996
Last Reported 2011
Negated 5
Speculated 4
Reported most in Body
Documents 129
Total Number 133
Disease Relevance 60.15
Pain Relevance 63.10

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

signal transduction (Cnr2) plasma membrane (Cnr2) signal transducer activity (Cnr2)
Anatomy Link Frequency
brain 24
microglial cells 11
cerebellum 7
spinal cord 6
neuronal 6
Cnr2 (Mus musculus)
Cnr2 - Q63R (1)
Pain Link Frequency Relevance Heat
agonist 2218 100.00 Very High Very High Very High
depression 2160 100.00 Very High Very High Very High
Cannabinoid 1870 100.00 Very High Very High Very High
antagonist 1290 100.00 Very High Very High Very High
Inflammation 592 100.00 Very High Very High Very High
Cannabinoid receptor 460 100.00 Very High Very High Very High
Opioid 143 100.00 Very High Very High Very High
opioid receptor 74 100.00 Very High Very High Very High
alcohol 1634 99.92 Very High Very High Very High
Pain 547 99.80 Very High Very High Very High
Disease Link Frequency Relevance Heat
Depression 2215 100.00 Very High Very High Very High
INFLAMMATION 707 100.00 Very High Very High Very High
Diabetes Mellitus 924 99.84 Very High Very High Very High
Pain 620 99.80 Very High Very High Very High
Targeted Disruption 430 99.76 Very High Very High Very High
Gliosis 10 99.56 Very High Very High Very High
Demyelinating Disease 396 99.44 Very High Very High Very High
Nervous System Injury 162 99.26 Very High Very High Very High
Alcohol Addiction 304 99.24 Very High Very High Very High
Cancer 83 99.20 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Therefore, neuronal expression of CB2-Rs had been ambiguous and controversial and its role in depression and substance abuse is unknown.


Gene_expression (expression) of CB2 in neuronal associated with depression and drug abuse
1) Confidence 0.78 Published 2008 Journal PLoS ONE Section Abstract Doc Link PMC2241668 Disease Relevance 0.78 Pain Relevance 0.50
For CB2 gene expression analysis, unpaired t test (GraphPad software) was used and p<0.05 is the accepted level of significant difference.
Gene_expression (expression) of CB2 gene
2) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.15 Pain Relevance 0
CB2 gene expression was then determined in animals that developed or did not develop alcohol preference.
Spec (determined) Gene_expression (expression) of CB2 gene associated with alcohol
3) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.11 Pain Relevance 0.31
In both figures 1 A and B, the control samples were set at 1.0 and the spleen was used as positive controls because it has the most abundant expression of CB2-Rs in mammals.
Gene_expression (expression) of CB2 in spleen
4) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.51 Pain Relevance 0.51
However, the neuronal expression of CB2-Rs in the brain and its role in depression and substance abuse is unknown.
Gene_expression (expression) of CB2 in brain associated with depression and drug abuse
5) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 1.42 Pain Relevance 0.69
These results showed that CB2 gene is present and expressed in the brains of naïve mice and in those exposed to chronic mild stress.
Gene_expression (expressed) of CB2 gene in brains associated with stress
6) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.46 Pain Relevance 0.49
While a number of laboratories were not able to detect the presence of CB2-Rs in healthy brains [9]–[11], there has been demonstration of CB2-R expression in rat microglial cells and other brain associated cells during inflammation [12]–[17].
Gene_expression (expression) of CB2-R in brain associated with inflammation
7) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 1.17 Pain Relevance 0.66
Mice that developed alcohol preference had reduced CB2 gene expression and chronic treatment with JWH015 a putative CB2-R agonist, enhanced alcohol consumption in stressed but not in control mice.
Gene_expression (expression) of CB2 gene associated with agonist and alcohol
8) Confidence 0.78 Published 2008 Journal PLoS ONE Section Abstract Doc Link PMC2241668 Disease Relevance 0.61 Pain Relevance 0.62
Therefore, very rigorous controls have been utilized in our study including 1) the pre-adsorption and co-incubation of the CB2-R antibody with the immunizing peptide resulting in blocking CB2 staining in the rat cerebellum, 2) in situ hybridization data show that CB2 gene is expressed in the cerebellum of wild type and not in the CB2 knockout mice, with CB2 gene being absent in the sense control in the wild type mice.
Neg (absent) Gene_expression (expressed) of CB2 gene in cerebellum associated with targeted disruption
9) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.18 Pain Relevance 0.18
Chronic treatment with heroin increased (p>0.05) while cocaine significantly (p<0.05) increased CB2 gene expression in mouse brain preparations using RT-PCR (Fig. 1C).
Gene_expression (expression) of CB2 gene in brain associated with cocaine
10) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.32 Pain Relevance 0.66
This tandem polymorphism is important as it has the potential to change function in the mature expressed cannabinoid CB2-R as demonstrated in the immune system by an in vitro assay [29].
Gene_expression (expressed) of CB2-R in immune system associated with cannabinoid
11) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.86 Pain Relevance 0.26
The expression of CB2 gene was compared by TaqMan real-time PCR with an ABI PRISM 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA), using the TaqMan gene expression assay for CB2 (Mm0043826_m1).


Gene_expression (expression) of CB2 gene
12) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.08 Pain Relevance 0.28
Furthermore, the expression levels of the CB1 gene using RT-PCR analysis was 100 times that of the CB2 gene expression levels with reference to the brain stem.
Gene_expression (expression) of CB2 gene in brain stem
13) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.13 Pain Relevance 0.06
In mice subjected to the chronic varying alcohol intake paradigm for alcohol preference, there was significant reduction CB2 gene expression in the striatum (P?
Gene_expression (expression) of CB2 gene in striatum associated with alcohol
14) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.14 Pain Relevance 0.66
We then determined more precisely the involvement of CB2 gene expression in separate groups of mice chronically treated with heroin (10 mg/kg) or cocaine (40 mg/kg) or those exposed to varying alcohol consumption.
Gene_expression (expression) of CB2 gene associated with alcohol and cocaine
15) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.43 Pain Relevance 0.55
CB2 gene expression and regulation by drug and alcohol treatment
Gene_expression (expression) of CB2 gene associated with alcohol
16) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.14 Pain Relevance 0.32
In the assay, we used spleen as positive control because of its high expression of CB2 gene and no cDNA in the TaqMan PCR reaction as negative control.
Gene_expression (expression) of CB2 gene in spleen
17) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0 Pain Relevance 0
The expression of CB2 gene was compared by TaqMan real-time PCR with an ABI PRISM 7900 HT Sequence Detection System (Applied Biosystems, Foster City, CA, USA), using the TaqMan gene expression assay for CB2 (Mm0043826_m1).


Gene_expression (expression) of CB2
18) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.09 Pain Relevance 0.26
In animals that developed alcohol preference, CB2 gene expression was down regulated in the midbrain and striatum suggesting the involvement of CB2-Rs in the effects drugs of abuse.
Gene_expression (expression) of CB2 gene in striatum associated with midbrain and alcohol
19) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.50 Pain Relevance 0.68
To investigate whether CB2-R gene targeting by CB2-R antisense oligonucleotide (oligo) modifies behavior, we determined whether inhibition of CB2 gene expression in the brain will alter mouse behavior in the elevated plus-maze test.
Gene_expression (expression) of CB2 gene in brain
20) Confidence 0.78 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2241668 Disease Relevance 0.37 Pain Relevance 0.32

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