INT67724

Context Info Confidence 0.51 First Reported 1996 Last Reported 2010 Negated 4 Speculated 5 Reported most in Body Documents 14 Total Number 18 Disease Relevance 5.99 Pain Relevance 3.62

isomerase activity (ISYNA1) cytoplasm (ISYNA1)

Anatomy Link Frequency monocytes 2 glial cells 2 astrocytes 2 platelets 2 bladder 2 ISYNA1 (Homo sapiens)

Pain Link Frequency Relevance Heat Bioavailability 4 99.44 Inflammation 136 98.64 substance P 12 98.46 headache 8 98.16 interstitial cystitis 5 97.96 Migraine 11 97.36 cytokine 46 94.36 local anesthetic 2 93.44 Inflammatory mediators 11 92.08 agonist 4 91.04

Disease Link Frequency Relevance Heat Infection 49 98.84 INFLAMMATION 184 98.64 Headache 17 98.16 Interstitial Cystitis 5 97.96 Injury 9 97.16 Cystitis 1 96.60 Migraine Without Aura 1 96.56 Sepsis 9 96.04 Dengue 34 95.40 Endotoxemia 6 95.32

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

The bioavailability of NO and the activity and expression of iNOS are regulated by anesthetic agents.

Regulation (regulated) of Gene_expression (expression) of iNOS associated with bioavailability

1) Confidence 0.51 Published 2008 Journal J Anesth Section Abstract Doc Link 18500613 Disease Relevance 0.44 Pain Relevance 0.25

L-NMMA did not significantly affect the expression of IL-1beta, iNOS and COX-2.

Neg (not) Regulation (affect) of Gene_expression (expression) of iNOS

2) Confidence 0.36 Published 2002 Journal Osteoarthr. Cartil. Section Body Doc Link 12127835 Disease Relevance 0 Pain Relevance 0

Characterization of DENV target cells, DENV-Ag detection and iNOS expression in monocytes after in vitro infection

Regulation (detection) of Gene_expression (expression) of iNOS in monocytes associated with infection

3) Confidence 0.33 Published 2005 Journal BMC Infect Dis Section Body Doc Link PMC1208887 Disease Relevance 0.54 Pain Relevance 0

Based on the literature reported so far, the effects of anesthetics on iNOS expression and activity under conditions of inflammation are controversial, with the observed effects depending on the experimental methods and animal species used.

Regulation (effects) of Gene_expression (expression) of iNOS associated with inflammation

4) Confidence 0.22 Published 2008 Journal J Anesth Section Abstract Doc Link 18500613 Disease Relevance 0.75 Pain Relevance 0.32

To further evaluate whether the inhibition of LPS-stimulated NO and PGE2 production by AFW was mediated by the regulation of iNOS and COX-2 gene expression, RT-PCR analyses were performed.

Spec (whether) Regulation (regulation) of Gene_expression (expression) of iNOS

5) Confidence 0.16 Published 2010 Journal Journal of Biomedicine and Biotechnology Section Body Doc Link PMC2846352 Disease Relevance 0.28 Pain Relevance 0.17

Therefore, the inhibitory effect of AFW on iNOS and COX-2 gene expression is one possible mechanism for the anti-inflammatory action of AFW.

Regulation (effect) of Gene_expression (expression) of iNOS associated with inflammation

6) Confidence 0.16 Published 2010 Journal Journal of Biomedicine and Biotechnology Section Body Doc Link PMC2846352 Disease Relevance 0.26 Pain Relevance 0.13

Thus, in the current study we investigated whether inducible NO synthase (iNOS) mediated NO production is altered in the bladder of cats with a naturally occurring model of IC termed feline IC (FIC).

Spec (whether) Regulation (altered) of Neg (NO) Gene_expression (production) of iNOS in bladder associated with interstitial cystitis

7) Confidence 0.14 Published 2005 Journal J. Urol. Section Abstract Doc Link 15643277 Disease Relevance 0.56 Pain Relevance 0.46

The nNOS and iNOS are predominantly soluble enzymes whereas eNOS

Regulation (soluble) of Gene_expression (enzymes) of iNOS

8) Confidence 0.12 Published 2004 Journal Journal of Biomedicine and Biotechnology Section Body Doc Link PMC555773 Disease Relevance 0.16 Pain Relevance 0.16

Nitric oxide synthase (eNOS and iNOS) expression in platelets and the effects of ONOO- on membrane Na+/K+-ATPase activity and membrane fluidity were also evaluated.

Regulation (evaluated) of Gene_expression (expression) of iNOS in platelets

9) Confidence 0.09 Published 2005 Journal Cephalalgia Section Abstract Doc Link 15839850 Disease Relevance 0.87 Pain Relevance 0.82

Pathological increases in cellular NO levels due to an increase in iNOS expression or overactivation of eNOS and nNOS result in the formation of peroxynitrite (ONOO-) radical [67].

Regulation (overactivation) of Gene_expression (expression) of iNOS

10) Confidence 0.08 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2875392 Disease Relevance 0.13 Pain Relevance 0

While a weak correlation between K1 and EC50 values for IPs production could be found (r = 0.70), an excellent correlation could be demonstrated between their affinities (K1) and their potencies (EC50) for cAMP production (r = 0.97).

Regulation (values) of Gene_expression (production) of IPs

11) Confidence 0.07 Published 1996 Journal Bioorg. Med. Chem. Section Abstract Doc Link 9022979 Disease Relevance 0.05 Pain Relevance 0.32

As already quoted for septide, [pGlu6, Pro9]SP(6-11), discrepancies are observed between affinity (K1) and activity (EC50) values for IPs production.

Regulation (values) of Gene_expression (production) of IPs associated with substance p

12) Confidence 0.07 Published 1996 Journal Bioorg. Med. Chem. Section Abstract Doc Link 9022979 Disease Relevance 0.06 Pain Relevance 0.37

Since LPS-induced iNOS and COX-2 expression is primarily regulated by NF-?

Regulation (regulated) of Gene_expression (expression) of iNOS

13) Confidence 0.06 Published 2007 Journal Mediators of Inflammation Section Body Doc Link PMC1874678 Disease Relevance 0.06 Pain Relevance 0

However, we did not observe the expression of iNOS or nNOS (protein) as well as no production of NO (function) in our model (data not shown).

Neg (not) Spec (observe) Regulation (observe) of Gene_expression (expression) of iNOS

14) Confidence 0.06 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2920336 Disease Relevance 0.14 Pain Relevance 0

In this study, we investigated the inhibitory effects of inflexinol on LPS-induced NO production and expression of iNOS, COX-2 in RAW 264.7 cells and astrocytes.

Spec (investigated) Regulation (effects) of Neg (NO) Gene_expression (expression) of iNOS in astrocytes

15) Confidence 0.04 Published 2007 Journal Mediators of Inflammation Section Body Doc Link PMC1874678 Disease Relevance 0.95 Pain Relevance 0.38

At the gene level, the expression of iNOS is largely regulated

Regulation (regulated) of Gene_expression (expression) of iNOS

16) Confidence 0.04 Published 2007 Journal Mediators of Inflammation Section Body Doc Link PMC1874678 Disease Relevance 0.06 Pain Relevance 0

The results demonstrate key roles for ERK and p38 MAP kinase cascades in the transcriptional and post-transcriptional regulation of iNOS and TNFalpha gene expression in endotoxin-activated glial cells.

Regulation (regulation) of Gene_expression (expression) of iNOS in glial cells

17) Confidence 0.03 Published 1998 Journal J. Neurosci. Section Abstract Doc Link 9464988 Disease Relevance 0.22 Pain Relevance 0.10

In this study we have examined the regulation of TNFalpha and iNOS gene expression in endotoxin-stimulated primary glial cultures, focusing on the role of mitogen-activated protein (MAP) kinase cascades.

Spec (examined) Regulation (regulation) of Gene_expression (expression) of iNOS

18) Confidence 0.03 Published 1998 Journal J. Neurosci. Section Abstract Doc Link 9464988 Disease Relevance 0.47 Pain Relevance 0.14

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