INT67726

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Context Info
Confidence 0.75
First Reported 1997
Last Reported 2010
Negated 0
Speculated 2
Reported most in Body
Documents 36
Total Number 38
Disease Relevance 9.77
Pain Relevance 3.54

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (Notch1) Golgi apparatus (Notch1) plasma membrane (Notch1)
nucleus (Notch1) embryo development (Notch1) cytoplasm (Notch1)
Anatomy Link Frequency
Notch 6
band 2
stem cell 2
cleavage 1
platelet 1
Notch1 (Mus musculus)
Pain Link Frequency Relevance Heat
Central nervous system 48 100.00 Very High Very High Very High
Analgesic 9 100.00 Very High Very High Very High
Pain 10 99.08 Very High Very High Very High
opioid receptor 14 98.92 Very High Very High Very High
tail-flick 8 98.92 Very High Very High Very High
agonist 17 98.50 Very High Very High Very High
COX2 1 98.00 Very High Very High Very High
Antinociceptive 5 96.16 Very High Very High Very High
cytokine 89 94.92 High High
Demyelination 3 93.56 High High
Disease Link Frequency Relevance Heat
Stress 578 99.56 Very High Very High Very High
Thyroid Neoplasm 4 99.20 Very High Very High Very High
Pain 5 99.08 Very High Very High Very High
Cancer 126 98.40 Very High Very High Very High
Stroke 8 96.44 Very High Very High Very High
Apoptosis 84 95.76 Very High Very High Very High
Death 206 93.96 High High
Demyelinating Disease 126 93.56 High High
Neurodegenerative Disease 14 92.20 High High
Disease 232 90.24 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
It was previously reported that Notch1 is first synthesized as a 300-kDa precursor protein and that following the glycosylation step, Notch1 is cleaved by a furin-like convertase (site 1 cleavage; S1 cleavage) to form a heterodimer receptor composed of a 230-kDa extracellular domain (N1EC) and a 120-kDa transmembrane domain (N1™); and then the mature heterodimeric Notch1 is trafficked to the cell surface [17], [18].
Gene_expression (synthesized) of Notch1 in cleavage
1) Confidence 0.75 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0.14 Pain Relevance 0
Firstly, we performed Western blot analysis for detecting endogenous Notch1.
Gene_expression (detecting) of Notch1
2) Confidence 0.75 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0.39 Pain Relevance 0
To further assess the effect of periostin on Notch1 expression, we established retroviral infectants of a bone marrow-derived stromal cell line, ST2, by using the periostin-HA construct and an AcGFP construct for a control.
Gene_expression (expression) of Notch1 in stromal cell
3) Confidence 0.75 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0.28 Pain Relevance 0
Although this physical association of periostin with the Notch1 precursor might be appropriate, by the Western blot analysis, we detected the N1™ of heterodimeric Notch1 in the wild-type molar PDL, whereas we could not detect its precursor.
Gene_expression (detected) of Notch1 in molar
4) Confidence 0.75 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0 Pain Relevance 0
S1), suggesting that periostin might regulate Notch1 expression in various tissues that express periostin.
Gene_expression (expression) of Notch1
5) Confidence 0.75 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0 Pain Relevance 0
The sequences of the primers were as follow: for Notch1, 5?
Gene_expression (for) of Notch1
6) Confidence 0.65 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0 Pain Relevance 0.07
Periostin physically associates with Notch1 precursor at its EGF repeats
Gene_expression (precursor) of Notch1
7) Confidence 0.65 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0.17 Pain Relevance 0
In the result, although we could not detect Notch1 precursor, we did detect the 120-kDa N1™ band of S1-cleaved heterodimeric Notch1, indicating that Notch1 was expressed in the molar PDL (Fig. 2A).
Gene_expression (expressed) of Notch1 in band
8) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0 Pain Relevance 0
Our present results provide important insights into the regulation of Notch1 expression under the stress condition, and into periostin as a regulator of Notch1 expression, which is another unidentified function of periostin.


Gene_expression (expression) of Notch1 associated with stress
9) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0.65 Pain Relevance 0.03
In the result, although we could not detect Notch1 precursor, we did detect the 120-kDa N1™ band of S1-cleaved heterodimeric Notch1, indicating that Notch1 was expressed in the molar PDL (Fig. 2A).
Gene_expression (expressed) of Notch1 in band
10) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0 Pain Relevance 0
We first examined the Notch1 expression level by Western blot analysis using antibody against the C-terminal part of Notch1.
Spec (examined) Gene_expression (expression) of Notch1
11) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0 Pain Relevance 0
Our present results provide important insights into the regulation of Notch1 expression under the stress condition, and into periostin as a regulator of Notch1 expression, which is another unidentified function of periostin.


Gene_expression (expression) of Notch1 associated with stress
12) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0.58 Pain Relevance 0.03
We immunoprecipitated periostin using an anti-Myc antibody from the another whole-cell lysates of HEK293T transfectants that co-expressed Notch1-FLAG and periostin-Myc constructs and then performed Western blot analysis using an antibody raised against the extracellular region of rodent Notch1.
Gene_expression (expressed) of Notch1
13) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0 Pain Relevance 0
This result suggests that periostin affected Notch1 expression in post-transcriptional manner.
Gene_expression (expression) of Notch1
14) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0 Pain Relevance 0
This observation suggests that Notch1 proteins were synthesized in the Pn?
Gene_expression (ns were syn) of Notch1
15) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0 Pain Relevance 0
Different from this previous report, we propose that the Notch1 protein level is decreased under the oxidative stress condition but that periostin physically associates with the Notch1 precursor, and then maintains Notch1 protein expression and subsequent signaling under this stress condition.
Gene_expression (expression) of Notch1 protein associated with stress
16) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0.84 Pain Relevance 0.06
This result supports the periostin function for maintenance of Notch1 protein shown in Fig. 3A, suggesting that periostin maintains both Notch1 protein expression and subsequent signaling under the stress condition.
Gene_expression (expression) of Notch1 protein associated with stress
17) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0.57 Pain Relevance 0
We also examined Notch1 expression in the Pn?
Spec (examined) Gene_expression (expression) of Notch1
18) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2923609 Disease Relevance 0 Pain Relevance 0
Activated Notch1 analysis
Gene_expression (analysis) of Notch1
19) Confidence 0.54 Published 2010 Journal Head Neck Oncol Section Body Doc Link PMC2841166 Disease Relevance 0.96 Pain Relevance 0.13
-secretase inhibitors and expression of a Notch1 antisense reduce brain damage and improve neural function in that experimental model of ischemic stroke (Arumugam et al., 2006).
Gene_expression (expression) of Notch1 in neural associated with stroke
20) Confidence 0.43 Published 2010 Journal Frontiers in Aging Neuroscience Section Body Doc Link PMC2887037 Disease Relevance 1.69 Pain Relevance 0.12

General Comments

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