INT68133
From wiki-pain
|
|
|
|
|
Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
The cytoplasmatic and nuclear localization of the FGF-2 immunoreactivity [9] were taken into account in the discrimination of the neuronal and glial FGF-2 cell profiles. | |||||||||||||||
| |||||||||||||||
|
It was reported that the FGF-2 synthesized in the tongue may be retrogradaly transported to the hypoglossal nucleus thus acting as a target derived neurotrophic factor. | |||||||||||||||
| |||||||||||||||
|
Furthermore, a higher amount of FGF-2 was found in the nucleus of the reactive astrocytes of axotomized facial nuclei (Figure 4). | |||||||||||||||
| |||||||||||||||
|
Observations such as reduced b-FGF and VEGF secretion and MMP-9 regulation reveals that COX-2 inhibitors possess the potential to be exploited in the in vivo model to better understand their benefits as an adjuvant to the currently available chemotherapy for KS. | |||||||||||||||
| |||||||||||||||
|
The cytoplasmatic and nuclear localization of the FGF-2 immunoreactivity [9] were taken into account in the discrimination of the neuronal and glial FGF-2 cell profiles. | |||||||||||||||
| |||||||||||||||
|
Immunohistochemically, red-colored cells indicating PKH26-positive MSCs were surrounded by a green color suggesting local secretion of bFGF and VEGF from the MSCs (Fig. 3A and B).
| |||||||||||||||
| |||||||||||||||
|
MSCs have recently been shown to differentiate into multilineage cell types (8,9) and to secrete various cytokines, including bFGF and VEGF (10–13). | |||||||||||||||
| |||||||||||||||
|
In our study, bFGF and VEGF mRNA expressions in STZ-induced diabetic rats were upregulated in MSC-injected hind limb muscles, and MSCs maintained their viabilities and abilities to secrete bFGF and VEGF even 4 weeks after the transplantation. | |||||||||||||||
| |||||||||||||||
|
The level of therapeutic vasculogenesis induced by MSC transplantation was similar to that by endothelial progenitor cell transplantation, as we previously reported (16), and the differentiation or incorporation of MSCs to vessels was not observed, suggesting that neovasculalization induced by transplantation would be mediated through the actions of angiogenic factors such as VEGF and bFGF secreted from MSCs, which would explain the improvement of NBF. | |||||||||||||||
| |||||||||||||||
|
Therefore, VEGF and bFGF secreted by the transplanted MSCs would additively exert angiogenic and neurotrophic actions. | |||||||||||||||
| |||||||||||||||
|
We used immunohistochemistry to examine spinal localization of the cytokines, interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and the growth factors, basic fibroblast growth factor (bFGF), and transforming growth factor-beta1 (TGF-beta) at 3, 14, and 35 days following sciatic cryoneurolysis or 6 days following chronic constriction injury as compared with normal, unoperated rats. | |||||||||||||||
| |||||||||||||||
|
We used immunohistochemistry to examine spinal localization of the cytokines, interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and the growth factors, basic fibroblast growth factor (bFGF), and transforming growth factor-beta1 (TGF-beta) at 3, 14, and 35 days following sciatic cryoneurolysis or 6 days following chronic constriction injury as compared with normal, unoperated rats. | |||||||||||||||
| |||||||||||||||
|
Furthermore, colocalization of MSCs with VEGF and bFGF in the transplanted sites was confirmed. | |||||||||||||||
| |||||||||||||||
|
Scaffolds were characterized in vitro respect to their morphology, VEGF and bFGF release kinetics and bioactivity. | |||||||||||||||
| |||||||||||||||
|
Of particular interest for future studies are those proteins that are not normalized (Fgf2 and Crybb2) or only partially normalized (Cp, Cryba3, Lgals3, Stat3) by insulin treatment, as these persistent changes reflect the limitations of insulin to restore the retina to a nondiabetic state. | |||||||||||||||
| |||||||||||||||
|
It can be speculated that diabetic conditions such as hyperglycemia and impaired local microcirculation might cause or require the transplanted MSCs to more strongly produce and secrete bFGF and VEGF. | |||||||||||||||
| |||||||||||||||
|
Four weeks after the transplantation, MSCs maintained their viabilities and secreted angiogenic factors such as VEGF and bFGF at the injected sites. | |||||||||||||||
| |||||||||||||||
|
Local production and secretion of bFGF and VEGF. | |||||||||||||||
| |||||||||||||||
|
On the other hand, MSCs have been reported to secrete a wide array of cytokines, such as bFGF, VEGF, monocyte chemoattractant protein-1, and stem cell–derived factor-1 (12). | |||||||||||||||
| |||||||||||||||
|
In fact, MSC transplantation has been performed in the field of ischemic diseases, and it has been suggested that its plausible effects would be mediated largely through paracrine actions of locally released arteriogenic cytokines, including bFGF and VEGF (11,13,30). | |||||||||||||||
| |||||||||||||||
|
General Comments
This test has worked.