INT68681

Context Info Confidence 0.71 First Reported 1997 Last Reported 2011 Negated 1 Speculated 4 Reported most in Body Documents 132 Total Number 137 Disease Relevance 80.47 Pain Relevance 17.03

extracellular space (VEGFA) growth (VEGFA) extracellular region (VEGFA) proteinaceous extracellular matrix (VEGFA) cytoplasm (VEGFA)

Anatomy Link Frequency endothelial cell 4 myocardium 3 platelet 3 cardiac myocytes 3 inner ear 3 VEGFA (Homo sapiens)

Pain Link Frequency Relevance Heat metalloproteinase 138 100.00 chemokine 29 100.00 COX2 23 100.00 calcitonin gene related peptide 3 100.00 agonist 279 99.44 Pain 145 99.42 Inflammation 345 99.04 cytokine 200 98.88 palliative 100 98.84 ischemia 96 98.48

Disease Link Frequency Relevance Heat Hypoxia 365 100.00 Ectopic Pregnancy 95 99.96 Bursitis 13 99.88 Glioma 95 99.86 Respiratory Syncytial Virus 200 99.84 Cancer 2036 99.82 Sprains And Strains 69 99.70 Diabetes Mellitus 1069 99.68 Hepatocellular Cancer 126 99.48 Necrosis 51 99.48

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Using a co-culture system comprised of murine peritoneal macrophages and intervertebral disc tissue as a model of the acute phase of HD developed previously, an increase in macrophage VEGF protein and mRNA expression was observed upon exposure to disc tissue.

Transcription (expression) of VEGF protein in macrophage associated with intervertebral disk displacement

1) Confidence 0.71 Published 2002 Journal J. Orthop. Res. Section Abstract Doc Link 12038611 Disease Relevance 0.96 Pain Relevance 0.28

Hypoxia-induced transcription of VEGF mRNA is mediated by binding of hypoxia-inducible factor 1 (HIF-1) [7].

Transcription (transcription) of VEGF associated with hypoxia

2) Confidence 0.70 Published 2010 Journal Journal of Oncology Section Body Doc Link PMC2875768 Disease Relevance 1.36 Pain Relevance 0.05

We observed no significant deviation from the untreated mRNA levels for VEGF and bFGF during a 48 hour period after treatment with glucose alone or in combination with fluvastatin.

Transcription (levels) of VEGF

3) Confidence 0.69 Published 2003 Journal J Transl Med Section Body Doc Link PMC239962 Disease Relevance 0.38 Pain Relevance 0.16

Our study revealed a striking restriction of VEGF and bFGF mRNA expression in the diabetic sub-group of the atherosclerosis patient group with stable angina pectoris while the non diabetic sub-group displayed levels similar to the normal group.

Transcription (expression) of VEGF associated with stable angina pectoris, angina, atherosclerosis and diabetes mellitus

4) Confidence 0.69 Published 2003 Journal J Transl Med Section Body Doc Link PMC239962 Disease Relevance 1.65 Pain Relevance 0.38

To determine the effect of fluvastatin on the mRNA expression of bFGF and VEGF in monocytes of a healthy donor we performed kinetic analysis.

Transcription (expression) of VEGF in monocytes

5) Confidence 0.69 Published 2003 Journal J Transl Med Section Body Doc Link PMC239962 Disease Relevance 0.30 Pain Relevance 0

Real time reverse transcriptase polymerase chain reaction showed that morphine treatment (100 ng/ml) of hypoxic HUVECs resulted in a significant reduction in mRNA levels of VEGF(121) and VEGF(165) isoforms.

Transcription (levels) of VEGF associated with hypoxia and morphine

6) Confidence 0.69 Published 2001 Journal J. Mol. Cell. Cardiol. Section Abstract Doc Link 11735263 Disease Relevance 0.77 Pain Relevance 1.01

Real time reverse transcriptase polymerase chain reaction showed that morphine treatment (100 ng/ml) of hypoxic HUVECs resulted in a significant reduction in mRNA levels of VEGF(121) and VEGF(165) isoforms.

Transcription (levels) of VEGF associated with hypoxia and morphine

7) Confidence 0.69 Published 2001 Journal J. Mol. Cell. Cardiol. Section Abstract Doc Link 11735263 Disease Relevance 0.77 Pain Relevance 1.02

The intra assay variability of the mRNA measurements of our in vitro short term culture system was assessed by measuring the VEGF and bFGF mRNA in triplicate cultures each.

Transcription (measuring) of VEGF

8) Confidence 0.66 Published 2003 Journal J Transl Med Section Body Doc Link PMC239962 Disease Relevance 0.90 Pain Relevance 0.13

Quantitation of the mRNA for VEGF, bFGF and TGF-?

Transcription (mRNA) of VEGF

9) Confidence 0.66 Published 2003 Journal J Transl Med Section Body Doc Link PMC239962 Disease Relevance 1.39 Pain Relevance 0.21

Quantitation of the mRNA for VEGF, bFGF and TGF-?

Transcription (Quantitation) of VEGF

10) Confidence 0.66 Published 2003 Journal J Transl Med Section Body Doc Link PMC239962 Disease Relevance 1.40 Pain Relevance 0.22

Furthermore we analyzed in the above model the effect of high glucose levels found in diabetic individuals and statins found in the medication of all our patients on the mRNA levels of VEGF and bFGF during a 48 hour period.

Transcription (levels) of VEGF associated with diabetes mellitus

11) Confidence 0.66 Published 2003 Journal J Transl Med Section Body Doc Link PMC239962 Disease Relevance 0.60 Pain Relevance 0.05

Although both forms of the disease exhibited high levels of angiogenic activity, inflammatory breast cancer showed significantly higher mRNA expression of angiogenic and lymphangiogenic genes, including those encoding for VEGF-C and VEGF-D.

Transcription (expression) of VEGF associated with inflammation and disease

12) Confidence 0.63 Published 2010 Journal Clinical Medicine Insights. Oncology Section Body Doc Link PMC2883240 Disease Relevance 1.33 Pain Relevance 0.19

Although both forms of the disease exhibited high levels of angiogenic activity, inflammatory breast cancer showed significantly higher mRNA expression of angiogenic and lymphangiogenic genes, including those encoding for VEGF-C and VEGF-D.

Transcription (expression) of VEGF associated with inflammation and disease

13) Confidence 0.63 Published 2010 Journal Clinical Medicine Insights. Oncology Section Body Doc Link PMC2883240 Disease Relevance 1.33 Pain Relevance 0.19

One such example involves hypoxia-inducible factor-1, a transcription factor that regulates the expression of many angiogenesis- and glucose metabolism-related genes and in addition activates the transcription of VEGF in malignant gliomas.38,39 VEGF mRNA expression, as well as VEGF receptor expression, are well documented hypoxic induced changes in malignant gliomas.40

Transcription (transcription) of VEGF associated with hypoxia and glioma

14) Confidence 0.61 Published 2010 Journal OncoTargets and therapy Section Body Doc Link PMC2895775 Disease Relevance 1.06 Pain Relevance 0.08

One such example involves hypoxia-inducible factor-1, a transcription factor that regulates the expression of many angiogenesis- and glucose metabolism-related genes and in addition activates the transcription of VEGF in malignant gliomas.38,39 VEGF mRNA expression, as well as VEGF receptor expression, are well documented hypoxic induced changes in malignant gliomas.40

Transcription (expression) of VEGF associated with hypoxia and glioma

15) Confidence 0.61 Published 2010 Journal OncoTargets and therapy Section Body Doc Link PMC2895775 Disease Relevance 0.95 Pain Relevance 0.08

VEGF is not only involved in the regulation of neovascularization, it also has been proven in animal studies that if VEGF were suppressed at the level of mRNA or protein, corneal neovascularization was also decreased [2,14,27].

Transcription (level) of VEGF associated with corneal neovascularization

16) Confidence 0.59 Published 2010 Journal Korean Journal of Ophthalmology : KJO Section Body Doc Link PMC2916105 Disease Relevance 0.51 Pain Relevance 0.03

In this study, the PRP containing high amount of VEGF and PMP and PBMNCs without PBPMNs was called angiogenic factor-enriched PRP.

Transcription (amount) of VEGF

17) Confidence 0.55 Published 2010 Journal The Journal of Advanced Prosthodontics Section Body Doc Link PMC2984511 Disease Relevance 0.10 Pain Relevance 0

Analysis of VEGF transcripts by PCR, DNA cloning (Tischer et al. 1991) or RT-PCR (Poltorak et al. 1997) identified 5 isoforms with 121, 141, 145, 165 and 189 amino acids.

Transcription (transcripts) of VEGF

18) Confidence 0.53 Published 2007 Journal Genomic Med Section Body Doc Link PMC2276892 Disease Relevance 0.61 Pain Relevance 0.16

Endostatin also interacts with VEGF signal transduction by reducing VEGF-induced kinase (Erk1/2) phosphorylation [25].

Transcription (transduction) of VEGF

19) Confidence 0.53 Published 2008 Journal J Orthop Surg Section Body Doc Link PMC2397381 Disease Relevance 0.17 Pain Relevance 0.16

In an attempt to characterize factors affecting production and secretion of VEGF in EP, expression of VEGF mRNA and the VEGF receptors KDR and flt-1 were measured in implanted and non-implanted sites of the oviduct in women with EP41.

Transcription (expression) of VEGF in oviduct associated with ectopic pregnancy

20) Confidence 0.52 Published 2008 Journal Clinics Section Body Doc Link PMC2664731 Disease Relevance 0.88 Pain Relevance 0.05

General Comments

This test has worked.