INT68952

Context Info Confidence 0.62 First Reported 1997 Last Reported 2010 Negated 0 Speculated 2 Reported most in Body Documents 17 Total Number 19 Disease Relevance 5.64 Pain Relevance 1.78

cell proliferation (PCNA) nucleoplasm (PCNA) nucleolus (PCNA) nucleus (PCNA) DNA binding (PCNA) transcription factor binding (PCNA)

Anatomy Link Frequency bone marrow 2 gonadal 1 testis 1 internal 1 gingival epithelium 1 PCNA (Homo sapiens)

Pain Link Frequency Relevance Heat Opioid 1 97.48 Angina 8 95.00 Snapping jaw 27 93.88 metalloproteinase 6 89.84 Inflammation 83 88.36 Dopamine 2 85.92 bDMF 4 79.20 Glutamate 10 78.40 cerebral cortex 17 78.32 Somatostatin 2 75.52

Disease Link Frequency Relevance Heat Cancer 72 99.48 Volume Depletion And Dehydration 4 99.24 Hyperplasia 20 98.48 Adhesions 26 98.36 Apoptosis 48 97.76 Stable Angina Pectoris 7 95.00 Generalized Anxiety Disorder 40 94.84 Temporomandibular Joint Syndrome 27 93.88 Fibrosis 4 93.88 Acute Coronary Syndrome 8 93.08

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

PCNA Proliferating cell nuclear antigen

Localization (antigen) of PCNA

1) Confidence 0.62 Published 2005 Journal BMC Cancer Section Body Doc Link PMC1198222 Disease Relevance 0.82 Pain Relevance 0.39

Many PCNA-positive cells were localized in plaque areas, as follows: intimal hyperplasia, neovascularized lesions, lesions with macrophage clusters, and lesions near areas of disrupted internal elastic lamina.

Localization (localized) of PCNA in internal associated with hyperplasia

2) Confidence 0.61 Published 1997 Journal Hiroshima J. Med. Sci. Section Abstract Doc Link 9114565 Disease Relevance 1.44 Pain Relevance 0.59

Interestingly, there was a positive correlation between the increase in IL-6 and the increase in PCNA co-localization with Pax7 early in the post-intervention time-course.

Localization (localization) of PCNA

3) Confidence 0.53 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2696599 Disease Relevance 0.09 Pain Relevance 0.03

Celenligil – Nazlien et al. (2000) [47] investigated the localization of PCNA, which is a nuclear protein associated with the cell cycle in oral gingival epithelium and defined the age-related changes as to the PCNA-proliferative index (PI) in inflamed as well as healthy gingiva.

Spec (investigated) Localization (localization) of PCNA in gingival epithelium

4) Confidence 0.48 Published 2010 Journal The Open Dentistry Journal Section Body Doc Link PMC2874215 Disease Relevance 0.62 Pain Relevance 0

Moreover, there exists a great degree of colocalization between PCNA and PKH26 around the infarct penumbra.

Localization (colocalization) of PCNA

5) Confidence 0.27 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2974740 Disease Relevance 0 Pain Relevance 0.07

In order to explore if transplanted bone marrow cells might be a part of the proliferating cells seen around the infarct areas, we carried out double labeling analysis for colocalization of PCNA (visualized with Alexa-488 conjugated secondary antibody) and PKH26.

Localization (colocalization) of PCNA in bone marrow

6) Confidence 0.25 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2974740 Disease Relevance 0.08 Pain Relevance 0

To determine the colocalization of PCNA and DCX expression with transplanted BMMCs, additional sections were subject to double fluorescent labeling for PCNA or DCX with PKH26.

Spec (determine) Localization (colocalization) of PCNA

7) Confidence 0.25 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2974740 Disease Relevance 0.10 Pain Relevance 0

Measurement of PCNA-positive cells, chymase-positive cells, and mast cells

Localization (Measurement) of PCNA in mast cells

8) Confidence 0.24 Published 2009 Journal Molecular Vision Section Body Doc Link PMC2763124 Disease Relevance 0.34 Pain Relevance 0

Colocalization of transplanted bone marrow cells with PCNA

Localization (Colocalization) of PCNA in bone marrow

9) Confidence 0.23 Published 2010 Journal BMC Neurosci Section Body Doc Link PMC2974740 Disease Relevance 0.25 Pain Relevance 0

PCNA-immunopositive cells were detected in the gonadal/mesonephros complex at day 30 (Fig. 6d) and was predominantly localised to the seminiferous cords at the later gestational ages examined.

Localization (localised) of PCNA in mesonephros

10) Confidence 0.17 Published 2005 Journal Reprod Biol Endocrinol Section Body Doc Link PMC1291400 Disease Relevance 0 Pain Relevance 0

FBS- and PDGF-BB-stimulated intracellular Ras, MEK1/2, ERK1/2, proliferative cell nuclear antigen (PCNA), and Akt activations were significantly inhibited by lercanidipine; however, lercanidipine did not affect FBS- and PDGF-BB-induced STAT3 phosphorylation.

Localization (antigen) of PCNA

11) Confidence 0.16 Published 2009 Journal Pharmacol. Res. Section Abstract Doc Link 18973813 Disease Relevance 0.40 Pain Relevance 0.15

For antigen retrieval of PCNA (mouse IgG), the sections were heated in distilled water in an autoclave for 10 minutes.

Localization (retrieval) of PCNA

12) Confidence 0.12 Published 2010 Journal BMC Gastroenterol Section Body Doc Link PMC2912238 Disease Relevance 0 Pain Relevance 0.04

B, and PCNA were normally localized only in the synovial membrane of wild type TMJs.

Localization (localized) of PCNA

13) Confidence 0.10 Published 2010 Journal Anatomy & Cell Biology Section Abstract Doc Link PMC2998788 Disease Relevance 0.71 Pain Relevance 0.39

In a second step, CD3 and PCNA were visualized with donkey anti-goat, conjugated to Alexa Fluor 594 (Molecular probes, Eugene, OR).

Localization (visualized) of PCNA

14) Confidence 0.09 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2886834 Disease Relevance 0 Pain Relevance 0

Integrated optical density of Western blot reaction with antiserum directed against PCNA and ?

Localization (directed) of PCNA

15) Confidence 0.09 Published 2004 Journal Reprod Biol Endocrinol Section Body Doc Link PMC416489 Disease Relevance 0.17 Pain Relevance 0.04

Proliferation marker PCNA is localized in interstitial cells of postnatal rodent testis

Localization (localized) of PCNA in testis

16) Confidence 0.09 Published 2004 Journal Reprod Biol Endocrinol Section Body Doc Link PMC416489 Disease Relevance 0.26 Pain Relevance 0

This same protocol was followed for proliferating cell nuclear antigen (PCNA), a co-factor for DNA polymerase and a convenient endogenous marker for newly proliferated cells, immunofluorescence using mouse anti-PCNA (1?

Localization (marker) of PCNA

17) Confidence 0.07 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2995744 Disease Relevance 0 Pain Relevance 0

PCNA proliferating cell nuclear antigen

Localization (antigen) of PCNA

18) Confidence 0.07 Published 2004 Journal BMC Neurosci Section Body Doc Link PMC434499 Disease Relevance 0.36 Pain Relevance 0.08

PCNA-immunopositive cells were detected in the gonadal/mesonephros complex at day 30 (Fig. 6d) and was predominantly localised to the seminiferous cords at the later gestational ages examined.

Localization (localised) of PCNA in gonadal

19) Confidence 0.06 Published 2005 Journal Reprod Biol Endocrinol Section Body Doc Link PMC1291400 Disease Relevance 0 Pain Relevance 0

General Comments

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