INT69484

Context Info Confidence 0.70 First Reported 1997 Last Reported 2010 Negated 3 Speculated 2 Reported most in Body Documents 75 Total Number 78 Disease Relevance 12.86 Pain Relevance 52.27

Golgi apparatus (OPRM1) endoplasmic reticulum (OPRM1) plasma membrane (OPRM1) signal transducer activity (OPRM1)

Anatomy Link Frequency plasma 4 MCF-7 4 neuronal 4 lumen 4 ganglia 4 OPRM1 (Homo sapiens) OPRM1 - A118G (1)

Pain Link Frequency Relevance Heat opioid receptor 1832 100.00 Morphine 1228 100.00 Opioid 973 100.00 mu opioid receptor 310 100.00 MU agonist 53 100.00 nMDA receptor 10 100.00 Inflammation 266 99.88 antagonist 330 99.82 Central grey 93 99.68 narcan 423 99.62

Disease Link Frequency Relevance Heat Targeted Disruption 16 100.00 Nociception 131 99.92 INFLAMMATION 257 99.88 Pheochromocytoma 39 99.84 Urological Neuroanatomy 95 99.68 Neuroblastoma 71 99.28 Acquired Immune Deficiency Syndrome Or Hiv Infection 24 99.14 Adenocarcinoma 3 99.04 Carcinoma 1 98.76 Anaplastic Astrocytoma 13 98.16

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Overexpression of MOR1K in mammalian cells revealed that this 6TM receptor is not expressed at the cell membrane, but instead is retained in the intracellular compartment (Fig.1C).

Positive_regulation (Overexpression) of Gene_expression (Overexpression) of MOR1K

1) Confidence 0.70 Published 2010 Journal Mol Pain Section Body Doc Link PMC2894766 Disease Relevance 0.40 Pain Relevance 0.41

Taken together, these findings suggest that activation of constitutively expressed MOR inhibits microglial cell chemotaxis and support the notion of an anti-inflammatory role of MOR within the brain.

Positive_regulation (activation) of Gene_expression (expressed) of MOR in brain associated with inflammation and mu opioid receptor

2) Confidence 0.70 Published 1997 Journal J. Pharmacol. Exp. Ther. Section Abstract Doc Link 9152411 Disease Relevance 0.17 Pain Relevance 0.66

The results of our study indicate that, in MCF-7 cells, MOR gene expression is downregulated by opioid agonists and upregulated by opioid antagonists.

Positive_regulation (upregulated) of Gene_expression (expression) of MOR gene in MCF-7 associated with mu agonist, antagonist and opioid

3) Confidence 0.68 Published 2008 Journal Biochem. Cell Biol. Section Abstract Doc Link 18523482 Disease Relevance 0.15 Pain Relevance 1.00

Naloxone alone produced a slight increase in MOR gene expression.

Positive_regulation (increase) of Gene_expression (expression) of MOR gene associated with narcan

4) Confidence 0.68 Published 2008 Journal Biochem. Cell Biol. Section Abstract Doc Link 18523482 Disease Relevance 0.15 Pain Relevance 0.99

The aim of the study was to investigate the presence of opioid receptor types in human breast adenocarcinoma MCF-7 cells and to characterize the changes in MOR expression induced by opioid agonist and antagonist treatment.

Positive_regulation (induced) of Gene_expression (expression) of MOR in MCF-7 associated with adenocarcinoma, mu agonist, antagonist and opioid receptor

5) Confidence 0.67 Published 2008 Journal Biochem. Cell Biol. Section Abstract Doc Link 18523482 Disease Relevance 0.17 Pain Relevance 0.66

Retinoid-induced mu opioid receptor expression by phytohemagglutinin-stimulated U937 cells.

Positive_regulation (induced) of Gene_expression (expression) of mu opioid receptor associated with mu opioid receptor

6) Confidence 0.62 Published 2005 Journal J. Neurovirol. Section Title Doc Link 16036794 Disease Relevance 0.38 Pain Relevance 0.77

Retinoid X receptor (RXR) agonist and retinoic acid receptor (RAR) antagonist further increased MOR expression by the PHA-stimulated cells.

Positive_regulation (increased) of Gene_expression (expression) of MOR associated with antagonist, mu opioid receptor and agonist

7) Confidence 0.62 Published 2005 Journal J. Neurovirol. Section Abstract Doc Link 16036794 Disease Relevance 0.32 Pain Relevance 0.89

It was shown previously that MOP expression is not increased in PC12 cells exposed to ethanol [22], [27], suggesting that elevated MOP surface density is due to differences in trafficking and/or sorting of the existing pool of MOP.

Neg (not) Positive_regulation (increased) of Gene_expression (expression) of MOP associated with opioid receptor and pheochromocytoma

8) Confidence 0.59 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2602977 Disease Relevance 0.10 Pain Relevance 0.84

We find that chronic exposure of human saphenous vein, atria and internal thoracic artery endothelium to the human immunodeficiency virus surface glycoprotein gp120, results in an increase in endothelial mu opioid receptor expression (52%). gp120 acts, in this regard, as a proinflammatory cytokine (e.g. interleukin-1-alpha) by increasing endothelial mu opioid receptor expression.

Positive_regulation (increase) of Gene_expression (expression) of mu opioid receptor in endothelium associated with acquired immune deficiency syndrome or hiv infection, mu opioid receptor and cytokine

9) Confidence 0.59 Published 2001 Journal Int. J. Mol. Med. Section Abstract Doc Link 11445868 Disease Relevance 0.17 Pain Relevance 0.69

The MOR gene is tightly regulated at the level of transcription, and potential polymorphisms in its 5' regulatory region can cause individual variation in MOR gene expression, nociception, and opiate responses.

Positive_regulation (cause) of Gene_expression (expression) of MOR gene associated with nociception, mu opioid receptor and opiate

10) Confidence 0.59 Published 2001 Journal DNA Cell Biol. Section Abstract Doc Link 11506703 Disease Relevance 0.29 Pain Relevance 0.61

Real-time PCR indicated that the expression of MOR, DOR and KOR in hypertrophic scars was enhanced in comparison with normal skin.

Positive_regulation (enhanced) of Gene_expression (expression) of MOR in skin

11) Confidence 0.51 Published 2008 Journal Br. J. Dermatol. Section Body Doc Link 18284397 Disease Relevance 0.07 Pain Relevance 0

Be2C cells transfected with either MOR1 or MOR1K retained a significantly higher proportion of fluorescently-labeled naloxone in comparison with cells transfected with an empty vector control.

Positive_regulation (transfected) of Gene_expression (transfected) of MOR1 associated with narcan

12) Confidence 0.50 Published 2010 Journal Mol Pain Section Body Doc Link PMC2894766 Disease Relevance 0.13 Pain Relevance 0.46

A moderate increase in morphine-evoked Ca2+ levels was also observed in Be2C cells transfected with MOR1 or empty vector, however this was likely due to the high endogenous expression of MOR1K in this cell line (Fig.1B).

Positive_regulation (transfected) of Gene_expression (transfected) of MOR1 associated with morphine

13) Confidence 0.50 Published 2010 Journal Mol Pain Section Body Doc Link PMC2894766 Disease Relevance 0.17 Pain Relevance 0.87

For MOR1 expressing cells, maximum NO production (7.5 pM) occurred at ~50 sec after administration of 1 ?

Positive_regulation (occurred) of Gene_expression (expressing) of MOR1

14) Confidence 0.50 Published 2010 Journal Mol Pain Section Body Doc Link PMC2894766 Disease Relevance 0.08 Pain Relevance 1.25

Since activation of MOR1K results in the intracellular accumulation of Ca2+ and showed a tendency to increase cAMP levels, we examined whether MOR1K couples to G?

Spec (examined) Positive_regulation (activation) of Gene_expression (results) of MOR1K

15) Confidence 0.50 Published 2010 Journal Mol Pain Section Body Doc Link PMC2894766 Disease Relevance 0.38 Pain Relevance 0.65

Furthermore, COS1 cells transfected with MOR1K showed a substantial increase in intracellular Ca2+ release following morphine treatment, which was not observed in MOR1 expressing cells (Fig. 2B).

Positive_regulation (transfected) of Gene_expression (transfected) of MOR1K associated with morphine

16) Confidence 0.50 Published 2010 Journal Mol Pain Section Body Doc Link PMC2894766 Disease Relevance 0.15 Pain Relevance 0.54

These data suggest that human MOR1K expression is restricted to neuronal cells.

Positive_regulation (restricted) of Gene_expression (expression) of MOR1K in neuronal

17) Confidence 0.50 Published 2010 Journal Mol Pain Section Body Doc Link PMC2894766 Disease Relevance 0.50 Pain Relevance 0.46

For MOR1 expressing cells, maximum NO production (7.5 pM) occurred at ~50 sec after administration of 1 ?

Positive_regulation (For) of Gene_expression (expressing) of MOR1

18) Confidence 0.50 Published 2010 Journal Mol Pain Section Body Doc Link PMC2894766 Disease Relevance 0.08 Pain Relevance 1.23

Be2C cells transfected with either MOR1 or MOR1K retained a significantly higher proportion of fluorescently-labeled naloxone in comparison with cells transfected with an empty vector control.

Positive_regulation (transfected) of Gene_expression (transfected) of MOR1K associated with narcan

19) Confidence 0.50 Published 2010 Journal Mol Pain Section Body Doc Link PMC2894766 Disease Relevance 0.13 Pain Relevance 0.47

RESULTS: In this study, we demonstrate that after 12h cold exposure (4 degrees C from 24 degrees C), the estimated relative mu opiate receptor (MOR) gene expression in M. edulis pedal ganglia, measured by real-time PCR, did not differ significantly from the control group (1.23+/-0.25, p>0.05).

Positive_regulation (estimated) of Gene_expression (expression) of mu opiate receptor in ganglia

20) Confidence 0.49 Published 2003 Journal Neuro Endocrinol. Lett. Section Body Doc Link 12743536 Disease Relevance 0.09 Pain Relevance 0

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