INT70297

Context Info Confidence 0.75 First Reported 1997 Last Reported 2010 Negated 1 Speculated 0 Reported most in Body Documents 12 Total Number 13 Disease Relevance 4.85 Pain Relevance 2.33

mitochondrion (NEFH) cell death (NEFH) molecular_function (NEFH)

Anatomy Link Frequency neuron 3 smooth muscle 1 brains 1 motor neurons 1 neuronal 1 NEFH (Homo sapiens)

Pain Link Frequency Relevance Heat Opioid 22 97.56 adenocard 2 94.36 Spinal cord 9 93.56 cytokine 11 93.28 Inflammation 6 90.28 methadone 2 89.52 opiate 2 88.32 addiction 7 82.76 Neuritis 1 82.24 shoulder pain 1 62.16

Disease Link Frequency Relevance Heat Targeted Disruption 3 99.62 Ganglion Cysts 69 99.52 Glaucoma 273 98.76 Frailty 6 98.58 Cancer 26 98.34 Syndrome 6 98.08 Sprains And Strains 6 97.10 Sarcoma 11 95.52 INFLAMMATION 3 90.28 Neuroblastoma 6 90.12

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Immunohistochemistry of the tumour showed smooth muscle actin (SMA) to be focally positive, but Desmin, CD34, S-100, Neurofilament, EMA, CAM 5.2, CD117, CD21 and CD23 were negative.

Neg (negative) Gene_expression (negative) of Neurofilament in smooth muscle associated with cancer

1) Confidence 0.75 Published 2006 Journal Int Semin Surg Oncol Section Body Doc Link PMC1513591 Disease Relevance 1.56 Pain Relevance 0.11

These results suggest that NFH gene expression may be down regulated by an inhibitory feedback mechanism induced by perikaryal accumulations of NFs.

Gene_expression (expression) of NFH

2) Confidence 0.71 Published 1997 Journal Neurotoxicology Section Abstract Doc Link 9215990 Disease Relevance 0.11 Pain Relevance 0.18

Biochemical characterization by western blotting revealed that 1 and 5 mM concentrations of TOCP significantly inhibited the expression of neurofilament high molecular weight protein (NF-H), and that 5 mM TOCP inhibited expression of microtubule-associated protein 2c and tau protein, but not beta-actin.

Gene_expression (expression) of NF-H

3) Confidence 0.69 Published 2006 Journal Mol. Cell. Biochem. Section Abstract Doc Link 16909309 Disease Relevance 0.31 Pain Relevance 0.08

Decreased levels of nonphosphorylated NF-H (42-32%), NF-M (14-9%) and NF-L (30-29%) were found in the prefrontal cortex of opioid addicts compared with sex, age, and postmortem delay-matched controls.

Gene_expression (levels) of NF-H in cortex associated with opioid

4) Confidence 0.56 Published 2000 Journal J. Neurosci. Res. Section Abstract Doc Link 10900081 Disease Relevance 0.25 Pain Relevance 0.86

In contrast, increased levels of phosphorylated NF-H (58-41%) and NF-M (56-28%) were found in the same brains of opioid addicts.

Gene_expression (levels) of NF-H in brains associated with opioid

5) Confidence 0.56 Published 2000 Journal J. Neurosci. Res. Section Abstract Doc Link 10900081 Disease Relevance 0.23 Pain Relevance 0.86

Furthermore, forskolin is reported to induce the neuron-like morphology and expression of NSE, NFH, and Tuj1 in human MSCs cultured in serum-free conditions [61,62].

Gene_expression (expression) of NFH in neuron

6) Confidence 0.44 Published 2010 Journal BMC Cell Biol Section Body Doc Link PMC2867791 Disease Relevance 0 Pain Relevance 0.05

A1 hybrid neurons express neuron-specific markers such as neurofilament-L (NF-L), NF-M, NF-H, MAP-2, and beta tubulin III.

Gene_expression (express) of NF-H in neuron

7) Confidence 0.43 Published 2002 Journal Neurobiol. Dis. Section Abstract Doc Link 12460557 Disease Relevance 0.32 Pain Relevance 0.15

Axonal elongation and synaptic plasticity genes, including growth-associated protein 43 (GAP43), neurofilament heavy (NEFH), and neuritin 1 (NRN1), preferentially expressed by retinal ganglion cells, indicated an attempt to re-extend lost or damaged axons.

Gene_expression (expressed) of NEFH in retinal ganglion cells associated with ganglion cysts

8) Confidence 0.28 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2567987 Disease Relevance 0.43 Pain Relevance 0

GPNMB was chosen because it is a null mutation in the DBA/2J mouse strain, which develops iris pigment dispersion syndrome and iris stromal atrophy; the former was mapped to a premature stop mutation in GPNMB [64,65]; NEFH and GAP43 were chosen because they are markers of axonal regrowth [66,67], which has not been previously described in a primate glaucoma model.

Gene_expression (chosen) of NEFH in iris associated with syndrome, frailty, glaucoma and sprains and strains

9) Confidence 0.27 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2567987 Disease Relevance 0.53 Pain Relevance 0

Double staining with NEFH and protein disulfide isomerase (PDI), a marker of endoplasmic reticulum vesicles, suggested new protein synthesis in axons [68] and glial cells in the optic nerve (Figure 2E, F).

Gene_expression (staining) of NEFH in glial cells

10) Confidence 0.27 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2567987 Disease Relevance 0.89 Pain Relevance 0

Our experiments demonstrate that NI-hADSCs express increased immunoreactivities for neuronal markers Tuj1, MAP2, NFL, NFM, NFH, NSE, NeuN, GAP43, and SNAP25, as well as the increased mRNA expression of Tuj1, MAP2, NFL, NFM, NSE, GAP43, and SNAP25 compared to primary hADSCs (Figure 3), indicating that hADSCs differentiate into neural cells via bFGF and forskolin-mediated differentiation.

Gene_expression (express) of NFH in neuronal

11) Confidence 0.20 Published 2010 Journal BMC Cell Biol Section Body Doc Link PMC2867791 Disease Relevance 0 Pain Relevance 0.04

Low molecular weight neurofilament protein (NF-L), found in young immature neurons, increased considerably after 2 weeks of bFGF treatment and expression of mature NF forms, NF-M and NF-H, appeared after bFGF treatment.

Gene_expression (expression) of NF-H in neurons

12) Confidence 0.16 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2092394 Disease Relevance 0.06 Pain Relevance 0

For example, increased cell body size in transgenic rat motor neurons overexpressing the high molecular weight component of neurofilaments (NF-H) can be reduced by concurrrently increasing the expression of the low molecular weight component (NF-L) [19].

Gene_expression (overexpressing) of NF-H in motor neurons associated with targeted disruption

13) Confidence 0.11 Published 2005 Journal BMC Neurosci Section Body Doc Link PMC1079867 Disease Relevance 0.17 Pain Relevance 0

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