INT71782

Context Info Confidence 0.68 First Reported 1997 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 7 Total Number 8 Disease Relevance 3.17 Pain Relevance 1.37

mitochondrion (MAP2K1) Golgi apparatus (MAP2K1) cytoplasm (MAP2K1) cytosol (MAP2K1) signal transduction (MAP2K1) mitosis (MAP2K1)

Anatomy Link Frequency colon 1 epithelial cells 1 embryonic kidney 1 MAP2K1 (Homo sapiens)

Pain Link Frequency Relevance Heat Morphine 8 99.54 narcan 1 87.92 antagonist 3 86.24 agonist 1 84.32 aspirin 5 83.32 cINOD 13 81.88 opioid receptor 1 80.48 Inflammation 17 43.40 rheumatoid arthritis 6 5.00 addiction 4 5.00

Disease Link Frequency Relevance Heat Colon Cancer 49 99.06 Apoptosis 28 98.62 Breast Cancer 57 96.56 Death 2 93.36 Stress 2 92.80 Cancer 54 89.84 INFLAMMATION 23 77.08 Disease 9 66.44 Increased Venous Pressure Under Development 6 56.60 Takayasu Arteritis 100 52.60

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To determine whether inhibition of MEK/ERK activity is necessary for sulindac-induced apoptosis of human colon cancer cells, stable transfectants were created that express an activated MEK1 gene in HT29 cells.

Gene_expression (express) of MEK1 in colon associated with colon cancer and apoptosis

1) Confidence 0.68 Published 2004 Journal Cancer Res. Section Abstract Doc Link 15548677 Disease Relevance 1.23 Pain Relevance 0.14

Morphine enhanced the cellular levels of ERK1 (44 kDa), ERK2 (42 kDa), a 54-kDa ERK, MEK1 (45 kDa), and MEKK (78 kDa).

Gene_expression (levels) of MEK1 associated with morphine

2) Confidence 0.56 Published 1997 Journal J. Biol. Chem. Section Abstract Doc Link 9341110 Disease Relevance 0 Pain Relevance 0.81

Immunoblots and transcriptional reporter assays using specific inhibitors, as well as expression of constitutively active forms of MEK1 and MKK3, showed that c-IAP2 induction by cAMP is regulated predominantly through ERK1/2 and p38 MAPK and suggested involvement of p90 ribosomal protein S6 kinase and mitogen and stress response kinase-1 as well.

Gene_expression (expression) of MEK1 associated with stress

3) Confidence 0.42 Published 2004 Journal J. Biol. Chem. Section Abstract Doc Link 15078890 Disease Relevance 0.90 Pain Relevance 0.12

In these cells curcumin or demethoxycurcumin upregulated expression of cyclin-dependent kinase inhibitor 2D (p19INK4D) and downregulated expression of PCNA, HDAC2, MAP2K1 and PLAU.

Gene_expression (expression) of MAP2K1

4) Confidence 0.24 Published 2004 Journal J Carcinog Section Body Doc Link PMC421747 Disease Relevance 0.12 Pain Relevance 0.30

In prior work, we established human embryonic kidney epithelial cells engineered to express hTERT, the SV40 Early Region and an activated allele of MEK as a model system for demonstrating transformation activity of oncogenes, including AKT1 and IKBKE [28].

Gene_expression (express) of MEK in embryonic kidney

5) Confidence 0.08 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2813871 Disease Relevance 0.26 Pain Relevance 0

induces cell transformation in multiple cell types including human embryonic kidney and human mammary epithelial cells expressing hTERT, the SV40 Early Region, and constitutively active MEK (Fig. 6A), as well as in NIH 3T3 mouse fibroblasts (data not shown).

Gene_expression (expressing) of MEK in epithelial cells

6) Confidence 0.08 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2813871 Disease Relevance 0.37 Pain Relevance 0

Subsequently, AIBN (0.1 parts) and MEK (10 parts) were added, and the resulting product was allowed to stand for 3 h before the addition of MEK (50 parts).

Gene_expression (added) of MEK

7) Confidence 0.06 Published 2010 Journal DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes Section Body Doc Link PMC2993541 Disease Relevance 0.11 Pain Relevance 0

AIBN (0.1 parts) and MEK (70 parts) were then added, and the mixture was allowed to stand for 1 h.

Gene_expression (added) of MEK

8) Confidence 0.06 Published 2010 Journal DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes Section Body Doc Link PMC2993541 Disease Relevance 0.18 Pain Relevance 0

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