INT71788

Context Info Confidence 0.70 First Reported 1997 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 13 Total Number 13 Disease Relevance 4.90 Pain Relevance 1.66

cytosol (MAP3K1) cytoskeleton (MAP3K1) cytoplasm (MAP3K1)

Anatomy Link Frequency TK-1 1 cleavage 1 salivary gland 1 MAP3K1 (Homo sapiens) MAP3K1 - C1238V (1)

Pain Link Frequency Relevance Heat Morphine 8 99.54 positron emission tomography 48 97.68 interstitial cystitis 8 95.62 narcan 1 89.44 imagery 37 88.24 antagonist 1 87.76 opioid receptor 1 82.00 Kinase C 1 34.56 cva 2 32.40 peripheral neuropathy 5 12.16

Disease Link Frequency Relevance Heat Carcinoma 94 98.60 Skin Cancer 28 98.32 Shock 43 97.00 Cancer 153 96.44 Lung Cancer 10 96.32 Interstitial Cystitis 8 95.62 Non-small-cell Lung Cancer 17 95.20 Mastocytoma 1 93.00 Osteogenic Sarcomas 1 92.32 Stress 75 92.24

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

CBD-tagged wild type MEKK1 or the C1238V mutant were expressed in cells, purified on chitin beads and then treated with PEITC in vitro.

Gene_expression (expressed) of MEKK1

1) Confidence 0.70 Published 2007 Journal BMC Cancer Section Body Doc Link PMC2071920 Disease Relevance 0.20 Pain Relevance 0

As before, wild type MEKK1 but not C1238V MEKK1 was inhibited in vitro by Bio-ITC (lanes 4 and 7, middle panel), and wild type MEKK1 (but not C1238V MEKK1) was covalently labeled with Bio-ITC (lanes 4 and 7, top panel).

Gene_expression (labeled) of MEKK1 (C1238V)

2) Confidence 0.61 Published 2007 Journal BMC Cancer Section Body Doc Link PMC2071920 Disease Relevance 0.20 Pain Relevance 0

MEKK1 is protected from PEITC inhibition by an ATP analog

Gene_expression (protected) of MEKK1

3) Confidence 0.61 Published 2007 Journal BMC Cancer Section Body Doc Link PMC2071920 Disease Relevance 0.06 Pain Relevance 0

As before, wild type MEKK1 but not C1238V MEKK1 was inhibited in vitro by Bio-ITC (lanes 4 and 7, middle panel), and wild type MEKK1 (but not C1238V MEKK1) was covalently labeled with Bio-ITC (lanes 4 and 7, top panel).

Gene_expression (labeled) of MEKK1

4) Confidence 0.61 Published 2007 Journal BMC Cancer Section Body Doc Link PMC2071920 Disease Relevance 0.20 Pain Relevance 0

To determine the effect of isothiocyanates on MEKK1 activity, we expressed a full-length MEKK1 protein fused to a chitin binding domain (CBD) tag to allow purification of the protein by binding to chitin beads.

Gene_expression (expressed) of MEKK1

5) Confidence 0.54 Published 2007 Journal BMC Cancer Section Body Doc Link PMC2071920 Disease Relevance 0.17 Pain Relevance 0

These cells were chosen because they have relatively high expression of MEKK1 in comparison to other cell lines, allowing detection of the endogenous protein activity by immunoprecipitation and in vitro kinase assay.

Gene_expression (expression) of MEKK1

6) Confidence 0.54 Published 2007 Journal BMC Cancer Section Body Doc Link PMC2071920 Disease Relevance 0.33 Pain Relevance 0

The activity of MEKK1 expressed in cells was monitored using in vitro kinase assays to measure changes in catalytic activity.

Gene_expression (expressed) of MEKK1

7) Confidence 0.54 Published 2007 Journal BMC Cancer Section Abstract Doc Link PMC2071920 Disease Relevance 0.16 Pain Relevance 0

Morphine enhanced the cellular levels of ERK1 (44 kDa), ERK2 (42 kDa), a 54-kDa ERK, MEK1 (45 kDa), and MEKK (78 kDa).

Gene_expression (levels) of MEKK associated with morphine

8) Confidence 0.53 Published 1997 Journal J. Biol. Chem. Section Abstract Doc Link 9341110 Disease Relevance 0 Pain Relevance 0.82

Calcitriol promotes the expression of mitogen-activated protein kinase kinase kinase (MEKK-1) and the cleavage of caspase 3 when used in combination with cisplatin in SCC cells 18.

Gene_expression (expression) of MEKK-1 in cleavage associated with skin cancer

9) Confidence 0.11 Published 2010 Journal Journal of Cancer Section Body Doc Link PMC2938072 Disease Relevance 2.03 Pain Relevance 0

The general pattern of gene expression in IC and APF-treated cells suggested a less proliferative phenotype, with increased expression of E-cadherin, phosphoribosylpyrophosphate synthetase-associated protein 39, and SWI/SNF complex 170-kDa subunit, and decreased expression of vimentin, alpha2-integrin, alpha1-catenin, cyclin D1, and jun N-terminal kinase 1; these findings were confirmed for the structural gene products (E-cadherin, vimentin, alpha2-integrin, and alpha-catenin) by immunohistochemistry.

Gene_expression (expression) of kinase 1 associated with interstitial cystitis

10) Confidence 0.07 Published 2003 Journal Physiol. Genomics Section Abstract Doc Link 12847144 Disease Relevance 0.58 Pain Relevance 0.58

TK-1: thymidine kinase-1

Gene_expression (thymidine) of kinase-1 in TK-1

11) Confidence 0.04 Published 2007 Journal BMC Nucl Med Section Body Doc Link PMC1931583 Disease Relevance 0.26 Pain Relevance 0.27

2M), phosphoglycerate kinase 1 (PGK1), succinate dehydrogenase complex subunit A (SDHA), hypoxanthine-guanine phosphoribosyltransferase (HPRT), and ribosomal protein L13 (RPL13) genes, and expressed in terms of arbitrary units (a.u.). 2.5.

Gene_expression (expressed) of kinase 1

12) Confidence 0.02 Published 2010 Journal Journal of Nucleic Acids Section Body Doc Link PMC2911612 Disease Relevance 0 Pain Relevance 0

Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy Background

Gene_expression (Overexpression) of kinase 1 in salivary gland associated with carcinoma

13) Confidence 0.01 Published 2010 Journal BMC Cancer Section Title Doc Link PMC2949806 Disease Relevance 0.72 Pain Relevance 0

General Comments

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