INT71788
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
CBD-tagged wild type MEKK1 or the C1238V mutant were expressed in cells, purified on chitin beads and then treated with PEITC in vitro. | |||||||||||||||
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As before, wild type MEKK1 but not C1238V MEKK1 was inhibited in vitro by Bio-ITC (lanes 4 and 7, middle panel), and wild type MEKK1 (but not C1238V MEKK1) was covalently labeled with Bio-ITC (lanes 4 and 7, top panel). | |||||||||||||||
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MEKK1 is protected from PEITC inhibition by an ATP analog | |||||||||||||||
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As before, wild type MEKK1 but not C1238V MEKK1 was inhibited in vitro by Bio-ITC (lanes 4 and 7, middle panel), and wild type MEKK1 (but not C1238V MEKK1) was covalently labeled with Bio-ITC (lanes 4 and 7, top panel). | |||||||||||||||
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To determine the effect of isothiocyanates on MEKK1 activity, we expressed a full-length MEKK1 protein fused to a chitin binding domain (CBD) tag to allow purification of the protein by binding to chitin beads. | |||||||||||||||
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These cells were chosen because they have relatively high expression of MEKK1 in comparison to other cell lines, allowing detection of the endogenous protein activity by immunoprecipitation and in vitro kinase assay. | |||||||||||||||
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The activity of MEKK1 expressed in cells was monitored using in vitro kinase assays to measure changes in catalytic activity. | |||||||||||||||
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Morphine enhanced the cellular levels of ERK1 (44 kDa), ERK2 (42 kDa), a 54-kDa ERK, MEK1 (45 kDa), and MEKK (78 kDa). | |||||||||||||||
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Calcitriol promotes the expression of mitogen-activated protein kinase kinase kinase (MEKK-1) and the cleavage of caspase 3 when used in combination with cisplatin in SCC cells 18. | |||||||||||||||
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The general pattern of gene expression in IC and APF-treated cells suggested a less proliferative phenotype, with increased expression of E-cadherin, phosphoribosylpyrophosphate synthetase-associated protein 39, and SWI/SNF complex 170-kDa subunit, and decreased expression of vimentin, alpha2-integrin, alpha1-catenin, cyclin D1, and jun N-terminal kinase 1; these findings were confirmed for the structural gene products (E-cadherin, vimentin, alpha2-integrin, and alpha-catenin) by immunohistochemistry. | |||||||||||||||
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TK-1: thymidine kinase-1 | |||||||||||||||
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2M), phosphoglycerate kinase 1 (PGK1), succinate dehydrogenase complex subunit A (SDHA), hypoxanthine-guanine phosphoribosyltransferase (HPRT), and ribosomal protein L13 (RPL13) genes, and expressed in terms of arbitrary units (a.u.).
2.5. | |||||||||||||||
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Overexpression of sphingosine kinase 1 is associated with salivary gland carcinoma progression and might be a novel predictive marker for adjuvant therapy
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General Comments
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