INT80791

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Context Info
Confidence 0.78
First Reported 1999
Last Reported 2010
Negated 2
Speculated 2
Reported most in Body
Documents 113
Total Number 116
Disease Relevance 11.89
Pain Relevance 25.76

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

aging (Slc6a3) plasma membrane (Slc6a3) transmembrane transport (Slc6a3)
Anatomy Link Frequency
plasma 9
brain 7
cortex 4
striatum 3
prefrontal 1
Slc6a3 (Rattus norvegicus)
Pain Link Frequency Relevance Heat
Dopamine 4580 100.00 Very High Very High Very High
Kinase C 690 99.82 Very High Very High Very High
dopamine receptor 60 99.80 Very High Very High Very High
cocaine 137 99.52 Very High Very High Very High
monoamine 261 99.12 Very High Very High Very High
addiction 173 99.04 Very High Very High Very High
imagery 163 98.86 Very High Very High Very High
Inflammatory response 11 98.52 Very High Very High Very High
noradrenaline 94 98.40 Very High Very High Very High
local anesthetic 27 97.30 Very High Very High Very High
Disease Link Frequency Relevance Heat
Congenital Anomalies 85 99.84 Very High Very High Very High
Sprains And Strains 196 99.80 Very High Very High Very High
Pheochromocytoma 19 99.70 Very High Very High Very High
Cognitive Disorder 95 99.22 Very High Very High Very High
Urological Neuroanatomy 39 99.14 Very High Very High Very High
Drug Dependence 130 99.04 Very High Very High Very High
Gliosis 80 98.76 Very High Very High Very High
INFLAMMATION 34 98.12 Very High Very High Very High
Targeted Disruption 163 97.98 Very High Very High Very High
Cancer 115 97.04 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
In heterologous cells expressing the dopamine transporter (DAT), simultaneous elevation of intracellular Na(+) and depolarization of the membrane with gramicidin reduced the potency of various DAT substrates, including dopamine, d-amphetamine, beta-phenethylamine, p-tyramine, and MPP(+), in inhibiting binding of the cocaine analog [(3)H]CFT, with the greatest reduction observed for d-amphetamine.
Gene_expression (expressing) of dopamine transporter associated with dopamine, cocaine and potency
1) Confidence 0.78 Published 2005 Journal Neuropharmacology Section Abstract Doc Link 16122767 Disease Relevance 0 Pain Relevance 0.35
In heterologous cells expressing the dopamine transporter (DAT), simultaneous elevation of intracellular Na(+) and depolarization of the membrane with gramicidin reduced the potency of various DAT substrates, including dopamine, d-amphetamine, beta-phenethylamine, p-tyramine, and MPP(+), in inhibiting binding of the cocaine analog [(3)H]CFT, with the greatest reduction observed for d-amphetamine.
Gene_expression (expressing) of DAT associated with dopamine, cocaine and potency
2) Confidence 0.78 Published 2005 Journal Neuropharmacology Section Abstract Doc Link 16122767 Disease Relevance 0 Pain Relevance 0.35
Regulation of DAT expression and trafficking by CPE
Gene_expression (expression) of DAT
3) Confidence 0.64 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.25
Coexpression of CPE with DAT changed the diffuse DAT expression throughout the cell to stronger plasma membrane localization and suggests the interaction occur intracellularlly.
Gene_expression (expression) of DAT in plasma
4) Confidence 0.64 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.08
Since no change in DA uptake affinity could be observed upon coexpression of CPE with DAT, the increase in Vmax may reflect a change in the population of cell surface DAT.
Gene_expression (coexpression) of DAT associated with dopamine
5) Confidence 0.64 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.21
In virtually all cells coexpressing DAT and CPE, DAT was largely found at the plasma membrane and colocalized with CPE (Fig. 3C).
Gene_expression (coexpressing) of DAT in plasma
6) Confidence 0.64 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.03
Thus, the increased surface expression of DAT by CPE may involve phosphorylation events.
Gene_expression (expression) of DAT
7) Confidence 0.64 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.12
Interestingly, CPE band did show a slightly lower on pulse-chase assay coexpressed with DAT, which deserved further investigation.
Gene_expression (coexpressed) of DAT in band
8) Confidence 0.64 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.12
In addition, immunofluorescence analysis demonstrated that co-expression of CPE and DAT facilitated surface translocation of both proteins.
Gene_expression (expression) of DAT
9) Confidence 0.64 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.18
This is further supported by immunofluorescence for Cre recombinase and TH in the Slc6a3 Cre mouse, where TH and Cre were shown to be expressed in the same cells (Figure 1C).
Gene_expression (expressed) of Slc6a3
10) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2797636 Disease Relevance 0.18 Pain Relevance 0.36
In 3h and J3d,the expressions of DAT mRNA in the HP group (157.00 +/- 3.55 approximately 145.15 +/- 3.69) were significantly lower than those of the LP group (150.69 +/- 3.12 approximately 138.84 +/- 3.99) (P = 0.000).
Gene_expression (expressions) of DAT mRNA
11) Confidence 0.56 Published 2008 Journal Zhong Nan Da Xue Xue Bao Yi Xue Ban Section Body Doc Link 18812656 Disease Relevance 0 Pain Relevance 0
OBJECTIVE: To investigate the expression of dopamine D2 receptors (D2R) and dopamine transportors (DAT) located in the medial prefrontal contex (mPFC) in high and low conditioned place preference (CPP) rats, and to unveil the possible mechanism leading to different CPP susceptibilities.
Spec (investigate) Gene_expression (expression) of DAT in prefrontal associated with dopamine
12) Confidence 0.56 Published 2008 Journal Zhong Nan Da Xue Xue Bao Yi Xue Ban Section Abstract Doc Link 18812656 Disease Relevance 0 Pain Relevance 0.21
Cell surface DAT may derive from newly synthesized DAT or from recycled DAT that have been re-inserted into the membrane after being internalized.
Gene_expression (synthesized) of DAT
13) Confidence 0.56 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0
As described in Fig. 2B, DAT-CT tail583–620 MG cotransfected with DAT alone did not change either the Km or Vmax of cellular [3H] DA uptake.
Gene_expression (cotransfected) of DAT associated with dopamine
14) Confidence 0.56 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.26
As depicted in Fig. 2C and 2D, the estimated affinity of DA at the ligand binding site of DAT in CPE coexpressing cells was not significantly different from that observed for cells expressing DAT alone (IC50 = 986 ± 24 nM in cells expressing DAT vs 1001 ± 87 nM in cells expressing DAT and CPE).
Gene_expression (expressing) of DAT associated with dopamine
15) Confidence 0.56 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.22
Coexpression of CPE with DAT changed the diffuse DAT expression throughout the cell to stronger plasma membrane localization and suggests the interaction occur intracellularlly.
Gene_expression (Coexpression) of DAT in plasma
16) Confidence 0.56 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.10
Coexpression CPE and DAT increased the Vmax of DAT without effects on its affinity
Gene_expression (Coexpression) of DAT
17) Confidence 0.56 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.12
However, coexpression of the MG encoding the peptide DAT-CT583–620 significantly inhibited the increasing effects of DAT-mediated DA translocation by CPE.
Gene_expression (coexpression) of DAT-CT583 associated with dopamine
18) Confidence 0.56 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.25
As shown in Fig 3, coexpression of CPE increased the levels of both total DAT and cell surface DAT to about ~20% (Fig. 3A, B).
Gene_expression (levels) of DAT
19) Confidence 0.56 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.18
M in cells expressing DAT vs 6.7 ± 1.6 ?
Gene_expression (expressing) of DAT
20) Confidence 0.56 Published 2009 Journal Mol Brain Section Body Doc Link PMC2687442 Disease Relevance 0 Pain Relevance 0.17

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