INT81088

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Context Info
Confidence 0.77
First Reported 1999
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 26
Total Number 60
Disease Relevance 17.80
Pain Relevance 15.07

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

signal transduction (ASIC1) transport (ASIC1) plasma membrane (ASIC1)
Anatomy Link Frequency
sensory neurons 4
neurons 4
CNS 3
papillae 2
Mouse 2
ASIC1 (Homo sapiens)
Pain Link Frequency Relevance Heat
ASIC 584 100.00 Very High Very High Very High
Root ganglion neuron 2 99.84 Very High Very High Very High
Pain 82 99.76 Very High Very High Very High
Central nervous system 48 99.68 Very High Very High Very High
sodium channel 93 99.60 Very High Very High Very High
cINOD 25 99.38 Very High Very High Very High
Abeta 2 98.90 Very High Very High Very High
Inflammation 43 98.76 Very High Very High Very High
Potency 2 98.60 Very High Very High Very High
Angina 2 98.40 Very High Very High Very High
Disease Link Frequency Relevance Heat
Polycystic Kidney Disease 20 99.92 Very High Very High Very High
Death 288 99.80 Very High Very High Very High
Toxicity 211 99.80 Very High Very High Very High
Pain 72 99.76 Very High Very High Very High
Ganglion Cysts 3 99.68 Very High Very High Very High
Acidosis 159 99.24 Very High Very High Very High
INFLAMMATION 45 98.76 Very High Very High Very High
Angina 2 98.40 Very High Very High Very High
Disease 141 97.60 Very High Very High Very High
Apoptosis 44 97.36 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Neuronal activity is accompanied by pH fluctuations and the widespread expression of ASIC channels throughout the CNS suggests that activation of those ion channels by local acidic transients might play a role in neurotransmission or neuromodulation.
Gene_expression (expression) of ASIC in CNS associated with central nervous system and asic
1) Confidence 0.77 Published 2001 Journal Adv. Exp. Med. Biol. Section Abstract Doc Link 11950145 Disease Relevance 0.37 Pain Relevance 0.51
Immunohistochemical analyses also revealed that ASIC2b, as well as ASIC2a, was expressed in a subpopulation of taste cells, and some of the cells displayed both ASIC2a and ASIC2b immunoreactivities.
Gene_expression (expressed) of ASIC2b
2) Confidence 0.73 Published 2004 Journal Nihon Shinkei Seishin Yakurigaku Zasshi Section Abstract Doc Link 15484827 Disease Relevance 0.06 Pain Relevance 0.03
The sour-normal subjects also expressed the ASIC1 gene and as an example, the results from Subject 65 are shown (Figure 4, Lanes M and N, representing amplification of the 936 bp and 1535 bp products respectively).


Gene_expression (expressed) of ASIC1
3) Confidence 0.70 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2754526 Disease Relevance 0 Pain Relevance 0.12
In this study, we used RT-PCR to search for evidence of expression of the ASIC, PKD and ENaC families in the anterior fungiform (taste) papillae, in both the sour-ageusics and in three normal subjects.
Gene_expression (expression) of ASIC in papillae associated with polycystic kidney disease, sodium channel and asic
4) Confidence 0.70 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2754526 Disease Relevance 0.26 Pain Relevance 0.22
Certain ASIC subunits are also or exclusively expressed in neurons of the central nervous system (CNS) where the role of those cation channels is as for yet unknown.
Gene_expression (expressed) of ASIC in CNS associated with central nervous system and asic
5) Confidence 0.67 Published 2001 Journal Adv. Exp. Med. Biol. Section Abstract Doc Link 11950145 Disease Relevance 0.42 Pain Relevance 0.53
Whole-cell patch-clamp recordings were performed on neonatal rat cultured dorsal root ganglion neurons and on ASIC isoforms expressed in CHO cells.
Gene_expression (expressed) of ASIC in neurons associated with ganglion cysts, root ganglion neuron and asic
6) Confidence 0.66 Published 2007 Journal J. Neurosci. Section Abstract Doc Link 18045919 Disease Relevance 0.37 Pain Relevance 0.48
Here, using whole-cell patch-clamp electrophysiology and reverse transcriptase-polymerase chain reaction (RT-PCR), we show that HEK293 cells, a commonly used cell line for the expression and characterisation of many ion channels, functionally express an endogenous proton-gated conductance attributable to the activity of human ASIC1a.
Gene_expression (express) of ASIC1a
7) Confidence 0.65 Published 2001 Journal Pflugers Arch. Section Abstract Doc Link 11512022 Disease Relevance 0.17 Pain Relevance 0.25
To test if PICK1's effect on ASIC1a surface expression requires their interaction, we also examined the effect of a PICK1 PDZ domain mutant, KD-AA, which has two critical residues in the PDZ domain of PICK1, Lys27 and Asp28, mutated to alanine and renders the mutated PICK1 unable to interact with ASIC1a [38,39].
Gene_expression (expression) of ASIC1a
8) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0.04
Both the PDZ and BAR domains are required for PICK1's effect on surface expression of ASIC1a, we therefore asked if the PDZ and BAR domain mutants abolish the potentiation effect of PICK1 on acid-induced cell death.
Gene_expression (expression) of ASIC1a associated with death
9) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.35 Pain Relevance 0
We therefore asked whether the effect of PICK1 on ASIC1a surface expression is also dependent on the BAR domain of PICK1.
Gene_expression (expression) of ASIC1a
10) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0.04
Given that PICK1 regulates trafficking of several membrane proteins, and that PICK1 directly interacts with ASIC1, we hypothesize here that PDZ and BAR domains of PICK1 cooperatively regulate ASIC1a surface expression.
Gene_expression (expression) of ASIC1a
11) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.23 Pain Relevance 0
This result indicates that lipid binding is required for PICK1-regulated surface expression of ASIC1a.
Gene_expression (expression) of ASIC1a
12) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0.04
We co-transfected ASIC1a with wild-type PICK1 or BAR-domain mutant PICK1 2K-E into HEK293T cells.
Gene_expression (transfected) of ASIC1a
13) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0.03
Co-expressing of PICK1 with mouse ASIC1a also increased the population of faster migrating ASIC1a (Figure 1D).
Gene_expression (expressing) of ASIC1a
14) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0
These results indicate that both the PDZ and BAR domains are required for PICK1 to regulate ASIC1a surface expression.


Gene_expression (expression) of ASIC1a
15) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0.03
ASIC1a and myc-PICK1 were transfected into COS7 cells using lipofectamine 2000 (Invitrogen).
Gene_expression (transfected) of ASIC1a
16) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0
PICK1-regulated surface expression of ASIC1a is dependent on the lipid binding of PICK1's BAR domain
Gene_expression (expression) of ASIC1a
17) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0 Pain Relevance 0.03
Our results showed that PICK1 regulates cell-surface expression of ASIC1a in a lipid binding-dependent manner.
Gene_expression (expression) of ASIC1a
18) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.24 Pain Relevance 0
Similar to earlier reports [11,44,45], we found that ASIC1a overexpression significantly increased cell death upon acid treatment (ASIC1a transfected cells: 145.8% ± 14.8%, 164.6% ± 8.9%, 187.0% ± 12.8% for 2, 4, 6 hours respectively. n = 7, * p < 0.05, ** p < 0.01 compared to control groups, Figure 4).
Gene_expression (overexpression) of ASIC1a associated with death
19) Confidence 0.64 Published 2010 Journal Mol Brain Section Body Doc Link PMC3018362 Disease Relevance 0.62 Pain Relevance 0
RT-PCR revealed that all these cell types expressed TRPV1 (VR1), ASIC1a, and ASIC3 subunits of proton-gated ion channels.
Gene_expression (expressed) of ASIC1a
20) Confidence 0.63 Published 2003 Journal Toxicol. Appl. Pharmacol. Section Abstract Doc Link 14554100 Disease Relevance 0.29 Pain Relevance 0.33

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