INT8362

Context Info Confidence 0.78 First Reported 1982 Last Reported 2009 Negated 1 Speculated 1 Reported most in Abstract Documents 53 Total Number 54 Disease Relevance 5.09 Pain Relevance 27.00

peptidase activity (Prss12)

Anatomy Link Frequency bile 6 PAG 3 brain 3 small intestine 1 gut 1 Prss12 (Rattus norvegicus)

Pain Link Frequency Relevance Heat Enkephalin 40 100.00 5HT 36 100.00 substance P 112 99.88 tetrodotoxin 61 99.84 Opioid 36 99.84 Bile 143 99.72 Morphine 11 99.32 agonist 85 99.08 GABAergic 7 98.98 gABA 24 98.86

Disease Link Frequency Relevance Heat Urological Neuroanatomy 60 99.68 Nociception 49 97.80 Stress 8 96.08 Peptic Ulcer 5 95.48 INFLAMMATION 15 94.96 Hypothermia 8 92.28 Pain 66 86.08 Neuropathic Pain 2 12.68 Ganglion Cysts 5 6.96 Injury 4 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

These results are consistent with differences in NT receptors localization in these three dopaminergic structures.

Localization (localization) of NT

1) Confidence 0.78 Published 1988 Journal Naunyn Schmiedebergs Arch. Pharmacol. Section Abstract Doc Link 3368012 Disease Relevance 0 Pain Relevance 0.37

The 5HT-releasing effect of NT was completely inhibited by tetrodotoxin suggesting that NT receptors involved in the control of 5-HT release are not located on 5-HT terminals.

Neg (not) Localization (located) of NT associated with tetrodotoxin and 5ht

2) Confidence 0.73 Published 1998 Journal Neuropeptides Section Abstract Doc Link 9845009 Disease Relevance 0.15 Pain Relevance 0.50

NT(1-13) and NT(8-13) increased (3H)5HT release with EC50 values in the nanomolar range and Emax values in the range of 100% of control, whereas D-tyr11-NT was inactive.

Localization (release) of NT associated with 5ht

3) Confidence 0.60 Published 1998 Journal Neuropeptides Section Abstract Doc Link 9845009 Disease Relevance 0.17 Pain Relevance 0.34

SR 48527 also antagonized NT induced (3H)5HT release with an IC50 value of 0.95+/-0.06 nM whereas the inactive R(-) enantiomer SR 49711 only inhibited this effect with IC50 value close to 10(-6)M.

Localization (release) of NT associated with 5ht

4) Confidence 0.60 Published 1998 Journal Neuropeptides Section Abstract Doc Link 9845009 Disease Relevance 0.16 Pain Relevance 0.42

NT(1-13) and NT(8-13) increased (3H)5HT release with EC50 values in the nanomolar range and Emax values in the range of 100% of control, whereas D-tyr11-NT was inactive.

Localization (release) of NT associated with 5ht

5) Confidence 0.60 Published 1998 Journal Neuropeptides Section Abstract Doc Link 9845009 Disease Relevance 0.17 Pain Relevance 0.34

The previous study described in detail the localization of immunohistochemical NT in the rat brain.

Localization (localization) of NT in brain

6) Confidence 0.53 Published 1982 Journal J. Comp. Neurol. Section Abstract Doc Link 7142439 Disease Relevance 0.23 Pain Relevance 0.13

The fact that all these responsive brain areas contain NT indicates that the microinjection of NT may be mimicking the effects of endogenously released NT, thus supporting the possible physiological significance of NT as an endogenous modulator of body temperature or nociceptive sensory information.

Localization (released) of NT in body associated with nociception

7) Confidence 0.53 Published 1982 Journal J. Comp. Neurol. Section Abstract Doc Link 7142439 Disease Relevance 0.41 Pain Relevance 0.25

Pretreatment of the 1% bile extract with the bile salt-sequestering resin cholestyramine (2%, wt/vol) abolished NT-LI release.

Localization (release) of NT in bile associated with bile

8) Confidence 0.44 Published 1994 Journal Endocrinology Section Abstract Doc Link 8299558 Disease Relevance 0 Pain Relevance 0.32

These results show that the tauro-conjugated forms of cholic and deoxycholic acid are strong stimulants of NT-LI release.

Localization (release) of NT

9) Confidence 0.44 Published 1994 Journal Endocrinology Section Abstract Doc Link 8299558 Disease Relevance 0 Pain Relevance 0.46

Bolus injections of neurotensin (NT) in the rat perfused heart elicited a transient, dose-dependent histamine release.

Localization (release) of NT in heart

10) Confidence 0.40 Published 1985 Journal Peptides Section Abstract Doc Link 2581231 Disease Relevance 0 Pain Relevance 0.23

The nicotinic receptor agonist DMPP (10(-4) M) had no significant effect on NT-LI release.

Localization (release) of NT associated with agonist

11) Confidence 0.39 Published 1991 Journal Regul. Pept. Section Abstract Doc Link 1674614 Disease Relevance 0 Pain Relevance 0.44

Our results indicate that additionally to the secretion of NT induced by cholinergic agents and bombesin, substance P and to a lesser extent Leu-enkephalin are capable of stimulating NT release in the rat.

Localization (release) of NT associated with enkephalin and substance p

12) Confidence 0.39 Published 1991 Journal Regul. Pept. Section Abstract Doc Link 1674614 Disease Relevance 0 Pain Relevance 0.63

G-amino-n-butyric acid (GABA), histamine, serotonin and dopamine administered at final concentrations up to 10(-5) M had no effect on NT-LI release.

Localization (release) of NT associated with dopamine, gaba and serotonin

13) Confidence 0.39 Published 1991 Journal Regul. Pept. Section Abstract Doc Link 1674614 Disease Relevance 0 Pain Relevance 0.65

Finally, bile salt-induced NT-LI release is not mediated by intramural nerves and is not dependent on the activation of calcium channels.

Localization (release) of NT in bile associated with bile and calcium channel

14) Confidence 0.39 Published 1994 Journal Endocrinology Section Abstract Doc Link 8299558 Disease Relevance 0 Pain Relevance 0.38

Luminal administration of crude ox bile extract (0.25-1.5%, wt/vol) produced a dose-dependent release of NT-like immunoreactivity (NT-LI), with a maximal effect after infusion of 1% bile extract (500% of basal).

Localization (release) of NT in bile associated with bile

15) Confidence 0.39 Published 1994 Journal Endocrinology Section Abstract Doc Link 8299558 Disease Relevance 0 Pain Relevance 0.31

Bile salts in the distal small intestine are strong stimulants of neurotensin (NT) release, but the underlying mechanisms are not known.

Localization (release) of NT in small intestine associated with bile

16) Confidence 0.39 Published 1994 Journal Endocrinology Section Abstract Doc Link 8299558 Disease Relevance 0 Pain Relevance 0.27

The maximal secretion of NT-LI was observed after infusion of 20 mM TC (400% of basal).

Localization (secretion) of NT

17) Confidence 0.39 Published 1994 Journal Endocrinology Section Abstract Doc Link 8299558 Disease Relevance 0 Pain Relevance 0.33

Taurodeoxycholate (20 mM) was as potent as TC in stimulating NT-LI release, but the threshold concentration of taurodeoxycholate for NT-LI secretion was lower than that of TC.

Localization (release) of NT

18) Confidence 0.39 Published 1994 Journal Endocrinology Section Abstract Doc Link 8299558 Disease Relevance 0 Pain Relevance 0.40

To investigate the functional relationship between the enteric nervous system and the intestinal neurotensin (N) cells, the release of neurotensin (NT) was measured upon vascular 8-min infusion periods of various neurotransmitters and neuropeptides in an isolated vascularly perfused rat jejunoileum.

Localization (release) of NT in enteric nervous system associated with neurotransmitter and neuropeptide

19) Confidence 0.34 Published 1991 Journal Regul. Pept. Section Abstract Doc Link 1674614 Disease Relevance 0 Pain Relevance 0.24

In contrast, the micellar form of oleic acid (20 and 100 mM) in bile extract (1%) or TC (20 mM) dose dependently reduced the release of NT-LI induced by bile extract or TC alone.

Localization (release) of NT in bile associated with bile

20) Confidence 0.34 Published 1994 Journal Endocrinology Section Abstract Doc Link 8299558 Disease Relevance 0 Pain Relevance 0.49

General Comments

This test has worked.