INT84596
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
This investigation focused on the possibility that this increase in free water clearance following administration of an alpha(2)-adrenoceptor agonist was associated with a reduction in medullary AQP-2 expression. | |||||||||||||||
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Kidney expression of AQP2 was decreased in CRF rats, both vehicle-treated (CRF-V) and candesartan-treated (CRF-C) | |||||||||||||||
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Thus, these studies all provide firm evidence for the decreased of vasopressin-regulated water channel AQP2 expression and the significant defects in the collecting duct of the kidneys from CRF. | |||||||||||||||
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The decreased AQP2 expression in CRF rats was not altered in response to candesartan treatment; 3) Candesartan treatment was associated with decreased NHE3 and TSC expression in CRF, which could be due to the Ang II AT1 receptor blockade and/or decreased aldosterone levels; and 4) BSC-1 expression was increased in both CRF groups and the increased expression of BSC-1 was more prominent in candesartan-treated CRF rats compared with vehicle-treated CRF rats. | |||||||||||||||
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Semi-quantitative immunoblotting of whole kidney protein samples from CRF rats (remnant kidney) and sham-operated rats (corresponding right whole kidney) demonstrated that CRF rats, both vehicle-treated (57±9% of sham-operated control level, p<0.05) and candesartan-treated (54±6% of sham-operated control level, p<0.05), had significantly decreased AQP2 expression compared with sham-operated controls (Fig. 3, Table 2). | |||||||||||||||
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Semiquantitative immunoblotting demonstrated decreased AQP2 expression in both CRF-C (54% of control levels) and CRF-V (57%), whereas BSC-1 expression was increased in both CRF groups. | |||||||||||||||
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In the present study, we further examine whether Ang II AT1 receptor blocker candesartan treatment in rats with CRF, where plasma vasopressin and AngII levels are elevated, could alter the downregulation of renal AQP2 expression and decreased urine concentration which were already seen in CRF rats (7). | |||||||||||||||
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In addition, the expression of several aquaporins including AQP1, AQP2, AQP3, and AQP5 decrease with aging [4951], and therefore AQP4 expression may also decrease with aging. | |||||||||||||||
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General Comments
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