INT85379

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Context Info
Confidence 0.55
First Reported 1999
Last Reported 2010
Negated 0
Speculated 2
Reported most in Body
Documents 19
Total Number 21
Disease Relevance 3.42
Pain Relevance 0.72

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

mitochondrion (Pdss1)
Anatomy Link Frequency
myocardium 1
HeLa 1
fibroblasts 1
Pdss1 (Mus musculus)
Pain Link Frequency Relevance Heat
Angina 5 98.56 Very High Very High Very High
Percutaneous transluminal coronary angioplasty 4 97.72 Very High Very High Very High
Intracerebroventricular 34 78.40 Quite High
Central nervous system 34 75.40 Quite High
Pain 2 25.00 Low Low
fibrosis 17 15.04 Low Low
Spinal cord 68 11.24 Low Low
anesthesia 17 5.00 Very Low Very Low Very Low
Inflammation 7 5.00 Very Low Very Low Very Low
alcohol 3 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Disease 106 99.56 Very High Very High Very High
Recurrence 1 99.00 Very High Very High Very High
Angina 5 98.56 Very High Very High Very High
Infection 41 98.52 Very High Very High Very High
Spinal Muscular Atrophy 544 96.48 Very High Very High Very High
Cardiovascular Disease 2 92.96 High High
Cancer 7 90.92 High High
Skin Cancer 1 84.44 Quite High
Targeted Disruption 17 81.48 Quite High
Squamous Cell Carcinoma 2 77.28 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
TpT was present in the blood of 20 patients in the first 24 hours after PTCA, and group B patients had higher mean TpT concentration; that could result from reperfusion of more ischaemic myocardium.
Gene_expression (present) of TpT in myocardium associated with percutaneous transluminal coronary angioplasty
1) Confidence 0.55 Published 1999 Journal Pol. Arch. Med. Wewn. Section Abstract Doc Link 10592726 Disease Relevance 0.58 Pain Relevance 0.57
TBT and TPT were detected in nearly all samples.
Gene_expression (detected) of TPT
2) Confidence 0.49 Published 2003 Journal Environ. Sci. Technol. Section Abstract Doc Link 12775042 Disease Relevance 0 Pain Relevance 0
SMN protein induction was measured using pIn711 ASO as baseline, and fold change over pM13 alone (triplicate mean set to 1) as expressions of trans-splicing efficiency.


Gene_expression (expressions) of trans-splicing
3) Confidence 0.24 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0 Pain Relevance 0
(Supplemental Figure S1A, S1B, see Methods) The ASO-expressing plasmids were co-transfected into HeLa cells with the SMN2 mini-gene and the pM13 which expresses the SMN trans-splicing RNA.
Gene_expression (expresses) of trans-splicing in HeLa
4) Confidence 0.24 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0 Pain Relevance 0
SMN trans-splicing was measured using fold change over pM13 set to 1 as expressions of trans-splicing efficiency.


Gene_expression (measured) of trans-splicing
5) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.09 Pain Relevance 0
Therefore, we initially examined SMN2 trans-splicing with the goal of devising strategies to enhance trans-splicing efficiency in vivo.
Spec (examined) Gene_expression (examined) of trans-splicing
6) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.31 Pain Relevance 0.07
A previously described slow-polymerase encoding plasmid was co-transfected with the SMN2 mini-gene and the plasmid expressing the SMN trans-splicing RNA [21].
Gene_expression (expressing) of trans-splicing
7) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.12 Pain Relevance 0
Semi-quantitative determinations via Cy3 labeled trans-splicing products were imaged using laser Fuji-Imager FLA5000 at 550 nm excitation range.
Gene_expression (products) of trans-splicing
8) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.06 Pain Relevance 0
In the case of trans-splicing RNAs designs such as, 5?
Gene_expression (designs) of trans-splicing
9) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.13 Pain Relevance 0
To examine trans-splicing in a more disease-specific context, similar co-transfections were performed in primary SMA patient fibroblasts, 3813 cells.
Spec (examine) Gene_expression (examine) of trans-splicing in fibroblasts associated with disease and spinal muscular atrophy
10) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.28 Pain Relevance 0
The endogenous trans-spliced product is amplified by SMN Exon 6 (+7) Forward and M13 Reverse.
Gene_expression (product) of trans-spliced
11) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0 Pain Relevance 0
Therefore, the competition between SMN2 cis-splicing and trans-splicing is likely reduced, providing a potential advantage to trans-splicing in the SMN context compared to other alternatively regulated exons that retain fully functional splice sites.
Gene_expression (cis-splicing) of trans-splicing
12) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.14 Pain Relevance 0
SMN trans-splicing was measured using fold change over pM13 set to 1 as expressions of trans-splicing efficiency.


Gene_expression (expressions) of trans-splicing
13) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.10 Pain Relevance 0
A single lead candidate was identified that significantly elevated trans-splicing levels: ASO pIn711.
Gene_expression (levels) of trans-splicing
14) Confidence 0.21 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0 Pain Relevance 0
The combination of ASO-tsRNA mechanism and a novel single vector platform produces a potent enhancement of basal trans-splicing.
Gene_expression (produces) of trans-splicing
15) Confidence 0.18 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.13 Pain Relevance 0
Additionally, in a novel application of ASO-In411 and tsRNAEX4 co-delivery targeting a upstream SMN intron 3 exon 4 splicing context produced enhancement of basal trans-splicing.
Gene_expression (produced) of trans-splicing
16) Confidence 0.18 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0 Pain Relevance 0
In vitro assays identified an enhancing antisense RNA and were constructed into a novel single vector system individually expressing the trans-splicing RNA and the antisense RNA.
Gene_expression (expressing) of trans-splicing
17) Confidence 0.18 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.50 Pain Relevance 0.07
(Invitrogen) Sequencing of gel purified trans-splicing SMN M13 positive cDNAs produced from in vivo murine RT-PCR was performed using 1.5 ng of hSMN Exon 6 Forward primer and on a ABI 3730 DNA Analyzer and Applied Biosystems Big Dye Terminator system and read by Chromat Freeware.


Gene_expression (produced) of trans-splicing
18) Confidence 0.18 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0 Pain Relevance 0
Consistent with the hypothesis, in the presence of the slow transcribing polymerase, trans-splicing was substantially increased over the typical level of trans-splicing previously observed (Figure 1B).
Gene_expression (presence) of trans-splicing
19) Confidence 0.09 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 0.05 Pain Relevance 0
In 1999, Raeppel et al. successfully synthesized the common trans-syn-trans perhydrobenz[e]indene moiety in the isomalabaricane-type terpenoids, which enabled the chemical synthesis of stelletins and stelliferins [91].
Gene_expression (synthesized) of trans-syn-trans
20) Confidence 0.02 Published 2010 Journal Marine Drugs Section Body Doc Link PMC2852841 Disease Relevance 0.33 Pain Relevance 0

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